Inhibition of pilocarpine-induced saliva secretion by adrenergic agonists in ICR mice.

The aim of the present study was to clarify the effects of the adrenoceptor agonist isoproterenol (IPR) on saliva secretion stimulated by the muscarinic receptor agonist pilocarpine (PILO) in mice. Mice were injected with either 0.5 mg/kg, i.p. PILO alone or simultaneously with 2 mg/kg, i.p., IPR to evaluate the inhibitory effects of adrenoceptor agonists on saliva secretion. The mechanisms underlying changes in saliva flow rate were evaluated by histological examination of aquaporin 5 (AQP5) and saliva flow rate using the adenylate cyclase (AC) inhibitor SQ22536 (0.25 mg per mouse, s.c.), which was administered 30 min prior to PILO and/or IPR. Saliva volume decreased significantly in the mice treated simultaneously with PILO + IPR compared with that in mice treated with PILO alone. Changes in the intracellular localization of AQP5 were seen in PILO + IPR-treated mice, and those changes were reversed by SQ22536 pretreatment. In addition, the decreased salivary flow rate in the PILO + IPR-treated mice was partially restored by SQ22536 pretreatment. There were no significant changes in intracellular calcium or ATP levels among the groups. The results of the present study suggest the existence of an inhibitory effect of the sympathetic nervous system on parasympathetic-stimulated salivary secretion from the salivary gland.

Third molar position: reliability of panoramic radiography.

PURPOSE: The purpose of the present study was to determine whether panoramic radiographs could predict physical contact between the mandibular third molar and the mandibular canal on limited cone-beam computed tomography, known as dental 3D-CT (3-dimensional computed tomography [3D-CT]). PATIENTS AND METHODS: The association of images between the panoramic radiograph and the dental 3D-CT was investigated in a cross-sectional study. Seventy-three lower third molars in 65 patients were examined. Findings of absence or presence of the white line of the mandibular canal wall on panoramic radiographs and contact or separation between the tooth and the mandibular canal on dental 3D-CT were compared. RESULTS: Absence of a superior white line on panoramic radiography was associated with an increased risk of contact between the third molar and the mandibular canal on dental 3D-CT, even when the effects of tooth position, age, and gender were taken into account. The multivariate adjusted odds ratio was 10.79. Women were more likely to have contact between the 2 structures on dental 3D-CT when their panoramic radiograph showed absence of the white line. CONCLUSIONS: Panoramic radiography is useful for predicting to a limited extent physical contact between the mandibular third molar and the mandibular canal on dental 3D-CT.