Multifaceted Assessment of Wastewater-Based Epidemiology for SARS-CoV-2 in Selected Urban Communities in Davao City, Philippines: A Pilot Study.

Over 60 countries have integrated wastewater-based epidemiology (WBE) in their COVID-19 surveillance programs, focusing on wastewater treatment plants (WWTP). In this paper, we piloted the assessment of SARS-CoV-2 WBE as a complementary public health surveillance method in susceptible communities in a highly urbanized city without WWTP in the Philippines by exploring the extraction and detection methods, evaluating the contribution of physico-chemical-anthropogenic factors, and attempting whole-genome sequencing (WGS). Weekly wastewater samples were collected from sewer pipes or creeks in six communities with moderate-to-high risk of COVID-19 transmission, as categorized by the City Government of Davao from November to December 2020. Physico-chemical properties of the wastewater and anthropogenic conditions of the sites were noted. Samples were concentrated using a PEG-NaCl precipitation method and analyzed by RT-PCR to detect the SARS-CoV-2 N, RdRP, and E genes. A subset of nine samples were subjected to WGS using the Minion sequencing platform. SARS-CoV-2 RNA was detected in twenty-two samples (91.7%) regardless of the presence of new cases. Cycle threshold values correlated with RNA concentration and attack rate. The lack of a sewershed map in the sampled areas highlights the need to integrate this in the WBE planning. A combined analysis of wastewater physico-chemical parameters such as flow rate, surface water temperature, salinity, dissolved oxygen, and total dissolved solids provided insights on the ideal sampling location, time, and method for WBE, and their impact on RNA recovery. The contribution of fecal matter in the wastewater may also be assessed through the coliform count and in the context of anthropogenic conditions in the area. Finally, our attempt on WGS detected single-nucleotide polymorphisms (SNPs) in wastewater which included clinically reported and newly identified mutations in the Philippines. This exploratory report provides a contextualized framework for applying WBE surveillance in low-sanitation areas.

The nexus of macroplastic and microplastic research and plastic regulation policies in the Philippines marine coastal environments.

The Philippines was reported as one of the top plastic polluters (macroplastics and microplastics (MPs)) to the marine coastal environment, which necessitated the development and enforcement of plastic regulation policies. However, the nexus between the growing research on macroplastic/MP and the plastic regulation policies in the country is unexplored. Current review suggests that macroplastic/MP research in the Philippines is still in its infancy owing to methodological and technological constraints to apportion the sources and fluxes, characterize macroplastics/MPs, and identify influencing socio-environmental factors. While government efforts are underway, it is also unclear if local researches on macroplastics/MPs were the basis in institutionalizing the existing plastic regulation policies. Therefore the nexus between the researches carried out on this problem and the policies enforced cannot be concluded. Overall, this review presents gaps on the macroplastic/MP research needing more work on the problem to establish a sound science plastic regulation policy.