Individual and molecular level effects of produced water contaminants on nauplii and adult females of Calanus finmarchicus.

In the Barents Sea region new petroleum fields are discovered yearly and extraction of petroleum products is expected to increase in the upcoming years. Despite enhanced technology and stricter governmental legislation, establishment of the petroleum industry in the Barents Sea may potentially introduce a new source of contamination to the area, as some discharges of produced water will be allowed. Whether the presence of produced water poses a risk to the Arctic marine life remains to be investigated. The aim of this study was to examine effects of exposure to several compounds found in produced water-a mixture of selected organic compounds (APW), radium-226 ((226)Ra), barium (Ba), and a scale inhibitor-on the copepod species Calanus finmarchicus. Experiments were performed using exposure concentrations at realistic levels based on those detected in the vicinity of known discharge points. The influence of lethal and sublethal effects on early life stages was determined and significantly lower survival in the APW exposure groups was found. In the Ba treatment the life stage development did not proceed to the same advanced stages as observed in the control (filtered sea water). The scale inhibitor and (226)Ra treatments showed no significant difference from control. In addition, adult females were exposed to APW, (226)Ra, and a mixture of the two. Both individual-level effects (egg production and feeding) and molecular-level effects (gene expression) were assessed. On the individual level endpoints, only treatments including APW produced an effect compared to control. However, on the molecular level the possibility that also (226)Ra induced toxicologically relevant effects cannot be ruled out.

Bioaccumulation of phenanthrene and benzo[a]pyrene in Calanus finmarchicus.

With petroleum exploration and development expanding in the Arctic (AMAP, 2007) there is a need to obtain additional information on the ecotoxicology of Arctic organisms. Here we perform 192 h laboratory exposure experiments on the keystone Arctic zooplankton species, Calanus finmarchicus. We trace the accumulation and depuration of two polycyclic aromatic hydrocarbons (PAHs): phenanthrene and benzo[a]pyrene (B[a]P) using (14)C labeled PAH compounds. Copepods were not fed during the experiment, limiting uptake to diffusion processes alone. The lighter PAH compound, phenanthrene, accumulated rapidly in C. finmarchicus, reaching steady state within 96 h. The heavier PAH compound, B[a]P, accumulated more slowly and steady state was not reached within the 192 h exposure period. As expected, the bioconcentration factor (BCF) for B[a]P was higher than for phenanthrene in accordance with a higher octanol/water partition coefficient for B[a]P (log K(ow)=6.04) compared to phenanthrene (log K(ow)=4.53). However, for both compounds, log BCF was lower than log K(ow) that may indicate active biotransformation and excretion of the selected PAH compounds. These findings on the bio-uptake kinetics for petroleum hydrocarbons are essential for evaluating the potential consequences of an oil spill in the Arctic.

Evaluation of 137Cs, 133Xe and 3H activity concentrations monitored in the Arctic atmosphere.

This paper provides a brief introduction to the Arctic atmospheric radioactivity monitoring network. A decade of monitoring results have shown the 137Cs background levels in Arctic air range from 0.05 to 1.50 µBq/m3. The monitoring stations have sufficient sensitivity to detect 137Cs brought to the atmosphere due to resuspension in local soil and reemissions from biomass burning in a daily temporal resolution. These observations can be used as tracers for atmospheric processes. The 133Xe measurements obtained at Yellowknife, Resolute and Spitsbergen could support other research into how air pollution problems arise across intercontinental distances. It will help develop and improve models capable of predicting the long-distance transport and deposition of trace gases in the Arctic. Rainwater monitoring data collected in Finnish Lapland since the 1960's indicate that 3H radioactivity concentrations reached natural background levels in early 2000s, typically around 1-2 Bq/L monthly, with an annual seasonal variation cycle consistent with the observed of other cosmogenic radionuclides.

Elevated natural radioactivity in undisturbed forest and mountain areas of arctic Norway - local geology, soil characteristics, and transfer to biota.

This study deals with the geology in areas close to a large unexploited uranium deposit and the impact of bedrock characteristics on levels of radionuclides and other elements in soil and biota. Factors influencing soil inventory and ecosystem transfer are discussed, focussing on 238U, 226Ra, and 210Pb. Field work was carried out in Salangen Valley in Northern Norway. Sampling stations for soil and biota covered different habitats - grassland, birch forest and low alpine heathland. The geological survey confirmed uranium-bearing minerals in granitic gneiss and pegmatites. There was large variation in the local occurrence of uranium, reflecting the irregular nature of the pegmatite. Activity concentrations of 238U, 226Ra, and 210Pb in surface soil were elevated at sites close to U-enhanced bedrock, compared to sites with other types of bedrock. Particularly high soil levels were found for 226Ra and 210Pb, whereas activity concentrations of 238U were more variable, depending of local soil characteristics. Levels of other natural radionuclides (40K, 232Th) merely increased with soil mineral content, and concentrations of heavy metals were generally low at all sites. External dose rate (1 m above ground surface) was closely correlated with 226Ra levels in soil. Plant levels of 238U and 226Ra varied by several orders of magnitude depending on soil level and plant species, whereas plant levels of 210Pb and 210Po were largely affected by aerial fallout. Berries generally contained lower levels of 238U and 226Ra than green plant parts. As was the case for plants, the levels of 238U in earthworms were strongly correlated with the respective concentrations in the soil. Soil-to-plant transfer was markedly higher for 226Ra than for 238U. For both radionuclides, a positive correlation was found between concentration ratios of V. myrtillus (heath) and soil organic matter content. The 238U concentration ratios for earthworms were generally two orders of magnitude higher than for plants.

Realism and usefulness of multispecies experiment designs with regard to application in radioecology: A review.

Multispecies experiments like microcosms and mesocosms are widely used in many fields of research but not in radioecology. In radioecology, size limitations are important as large experimental volumes involve problems with waste (radionuclides), or shielding, absorption and available space in gamma fields (often within a climate chamber). We have therefore performed a literature review (ISI Web of Science, n = 406) of the design and properties of multispecies effect studies <100 L in size and with three or more mentioned taxa in other research fields to assess their suitability to radioecology. Studies with more mentioned taxa assess structural ecosystem parameters more often than studies with fewer mentioned taxa, while the opposite trend is seen for indirect effects/interactions. Studies of indirect effects benefit from more replicates and longer experiments. Almost all studies assess some ecosystem level parameter but only a quarter take a holistic approach assessing both structural and functional as well as indirect effects. We find that most cosms are custom-made systems, rather than standardised designs. Unmanipulated cosms consist of excised portions of the natural environment with a higher number of mentioned taxa, high ecological complexity and high realism, but have a relatively low replicability. In contrast, standardised cosms with fewer mentioned taxa have less ecological complexity but much higher replicability. This literature review shows that smaller cosm sizes have similar ecological complexity (e.g. number of taxa and trophic levels) and experimental duration as larger sized cosms, allowing for ecologically-relevant investigations, despite their small size. We encourage multispecies radioecology studies, preferably with environmental relevant doses and sufficient detail on dosimetry.