Lysobacter segetis sp. nov., Isolated from Soil.

A Gram-negative, aerobic, motile by gliding, rod-shaped bacterium, strain 17J68-2T, was isolated from a soil sample taken from Jeju Island, Republic of Korea. The isolate displayed high 16S rRNA gene sequence similarity to the members of the genus Lysobacter in the family Lysobacteraceae, with Lysobacter humi FJY8T (98.4% similarity), Lysobacter xinjiangensis RCML-52T (98.3%), and Lysobacter mobilis 9NM-14T (98.1%) as closest phylogenetic neighbors. Growth of strain 17J68-2T occurred at 15-42 °C, pH 7-8, and in the presence of 0-1.0% NaCl. Draft genome was 2.94 Mb in size with G+C content of 70.5 mol%. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylethanolamine. Ubiquinone Q-8 was the predominant respiratory quinone and the major fatty acids were C16:0 iso (39.4%), summed feature 3 (C16:1ω7c/C16:1ω6c) (6.6%), C11:0 iso 3-OH (6.4%), C15:0 iso (6.4%), and C16:1 iso H (6.2%). The DNA-DNA relatedness between strain 17J68-2T and L. humi, L. xinjiangensis, and L. mobilis were 39.9, 39.4, and 25.3%, respectively. From these results, it is concluded that the novel isolate possesses sufficient characteristics to differentiate it from the most closely affiliated Lysobacter species, and strain 17J68-2T represents a novel species of the genus Lysobacter, for which the name Lysobacter segetis sp. nov. (=KCTC 62237T = JCM 33058T) is proposed.

Larkinella knui sp. nov., isolated from soil.

A Gram-stain-negative, motile by gliding, rod-shaped, aerobic bacterium, designated 15J6-3T6T, was isolated from a soil sample collected from Jeju Island, South Korea, and characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 15J6-3T6T belongs to the family Cytophagaceae and is related to Larkinella harenae 15J9-9T (93.9 % similarity), Larkinella arboricola Z0532T (93.6 %), Larkinella bovis M2TB15T (93.3 %), and Larkinella insperata LMG 22510T (93.3 %). The DNA G+C content of strain 15J6-3T6T was 50.6 mol%. The detection of phosphatidylethanolamine and an unidentified polar lipid as major polar lipids, menaquinone-7 as the predominant quinone, and C16 : 1ω5c, iso-C15 : 0, and iso-C17 : 0 3-OH as the major fatty acids also supports the affiliation of the isolate to the genus Larkinella. Based on its phenotypic properties and phylogenetic distinctiveness, we propose that strain 15J6-3T6T should be classified in the genus Larkinella as a representative of a novel species, for which the name Larkinella knui sp. nov. is proposed. The type strain is 15J6-3T6T (=KCTC 42998T=JCM 31989T).

Larkinella terrae sp. nov., isolated from soil on Jeju Island, South Korea.

A Gram-stain negative, non-motile, rod-shaped, aerobic bacterium, designated 15J8-8T, was isolated from a soil sample collected on Jeju Island, South Korea, and characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 15J8-8T belongs to the family Cytophagaceae and is related to Larkinella bovis M2TB15T (95.0%), 'Larkinella harenae' 15J9-9 (94.5%), Larkinella arboricola Z0532T (93.2%), and Larkinella insperata LMG 22510T (93.0%). The DNA G+C content of strain 15J8-8T was 50.5 mol%. The detection of phosphatidylethanolamine and two unidentified polar lipids as major polar lipids; menaquinone-7 as the predominant quinone; and C16:1 ω5c, C16:0 N alcohol, and iso-C15:0 as the major fatty acids also supported the affiliation of the isolate to the genus Larkinella. Based on its phenotypic properties and phylogenetic distinctiveness, strain 15J8-8T should be classified in the genus Larkinella as representative of a novel species, for which the name Larkinella terrae sp. nov. is proposed. The type strain is 15J8-8T (= KCTC 52001T = JCM 31990T).