RESUMO
Metal toxicity in crop plants is a matter of scientific concern. Therefore, in recent years efforts have been made to minimize metal toxicity in crop plants. Out of various strategies, priming of seedlings with certain chemicals, like e.g. donors of signaling molecules, nutrients, metabolites or plant hormones has shown encouraging results. However, mechanisms related with the priming-induced mitigation of metal toxicity are still poorly known. Hence, we have tested the potential of 2-oxoglutarate (2-OG) priming in enhancing the arsenate (AsV ) toxicity tolerance in tomato seedlings along with deciphering the probable role of nitric oxide (NO) in accomplishing this task. Arsenate decreased growth, endogenous NO and nitric oxide synthase-like activity but enhanced the accumulation of As, which collectively led to root cell death. Arsenate toxicity also decreased some photosynthetic characteristics (i.e. Fv /Fm, qP, Fv /F0 and Fm /F0 , and total chlorophyll content) but enhanced NPQ. However, priming with 2-OG alleviated the toxic effect of AsV on growth, endogenous NO, cell death and photosynthesis. Moreover, arsenate inhibited the activities of enzymes of nitrogen metabolism (i.e. nitrate reductase, nitrite reductase, glutamine synthetase and glutamine 2-oxoglutarate aminotransferase) but increased the activity of glutamate dehydrogenase and NH4 + content. Superoxide radicals, hydrogen peroxide, lipid peroxidation, protein oxidation and membrane damage increased upon AsV exposure, but the antioxidant enzymes (i.e. superoxide dismutase, catalase and glutathione-S-transferase) showed differential responses. Overall, our results showed that 2-OG is capable of alleviating AsV toxicity in tomato seedlings but the involvement of endogenous NO is probably required.
Assuntos
Arsênio , Solanum lycopersicum , Antioxidantes , Ácidos Cetoglutáricos , Óxido Nítrico , Estresse Oxidativo , Fotossíntese , PlântulaRESUMO
In plants, investigation on heavy metal toxicity and its mitigation by nutrient elements have gained much attention. However, mechanism(s) associated with nutrients-mediated mitigation of metal toxicity remain elusive. In this study, we have investigated the role and interrelation of glutathione (GSH) and hydrogen sulfide (H2 S) in the regulation of hexavalent chromium [Cr(VI)] toxicity in tomato (Solanum lycopersicum), pea (Pisum sativum) and brinjal (Solanum melongena) seedlings, supplemented with additional sulfur (S). The results show that Cr(VI) significantly reduced growth, total chlorophyll and photosynthetic quantum yield of tomato, pea and brinjal seedlings which was accompanied by enhanced intracellular accumulation of Cr(VI) in roots. Moreover, Cr(VI) enhanced the generation of reactive oxygen species in the studied vegetables, while antioxidant defense system exhibited differential responses. However, additional supply of S alleviated Cr(VI) toxicity. Interestingly, addition of l-buthionine sulfoximine (BSO, a glutathione biosynthesis inhibitor) further increased Cr(VI) toxicity even in the presence of additional S but GSH addition reverses the effect of BSO. Under similar condition, endogenous H2 S, l-cysteine desulfhydrase (DES) activity and cysteine content did not significantly differ when compared to controls. Hydroxylamine (HA, an inhibitor of DES) also increased Cr(VI) toxicity even in the presence of additional S but sodium hydrosulfide (NaHS, an H2 S donor) reverses the effect of HA. Moreover, Cr(VI) toxicity amelioration by NaHS was reversed by the addition of hypotaurine (HT, an H2 S scavenger). Taken together, the results show that GSH which might be derived from supplied S is involved in the mitigation of Cr(VI) toxicity in which H2 S signaling preceded GSH biosynthesis.