Identifying obstacles to reducing the use of antibiotics to control porcine proliferative enteropathy.

Proliferative enteropathy (PE) is an enteric disease of pigs that results in diarrhoea, reduced growth rate, reduced feed conversion efficiency and sometimes death. A survey of 13 pig veterinary practitioners in Australia was conducted to determine: (1) PE control strategies (antibiotics and vaccination), (2) how the efficacies of these strategies are evaluated and (3) how reliance on antibiotics could be reduced by increasing vaccine adoption. Antibiotics were routinely prescribed in the diets of weaner, grower and finisher pigs by 9/13, 10/13 and 8/13 veterinarians, respectively, if vaccination was not implemented. Water-soluble antibiotics were prescribed less frequently than in-feed antibiotics. Efficacy of control strategies was assessed most often through reduced clinical signs (diarrhoea, 'tail-ender' pigs, death) and reduced lesion incidence at abattoir postmortem inspection. Twelve practitioners had recommended PE vaccination to their clients in the previous 6 months. Barriers to continued vaccine implementation included perceived lack of efficacy for pigs housed in bedded systems, high cost relative to medication and difficulties in vaccinating pigs post-weaning.

Stimulation of protein synthesis and expansion of pig blastocysts by insulin in vitro.

Present evidence indicates that insulin may act as a growth factor during preimplantation development. This hypothesis has been tested on pig blastocysts by determining the effect of insulin on protein synthesis and blastocyst expansion over 24 h. Blastocysts were collected from superovulated gilts or sows on Day 5 or 6 and incubated overnight in a modified BMOC2 medium. Those that were cultured with 1.7 nM insulin had 14% larger radii, and were 36% more active in their incorporation of [3H]leucine (protein synthesis) than those that had been cultured in non-supplemented medium. There was a significant linear correlation between the rate of protein synthesis and the radius of blastocysts when all blastocysts and only those cultured with insulin were examined, but the correlation for the blastocysts in non-supplemented medium was just outside statistical significance. The regression coefficient for the insulin-treated blastocysts was 132% of that for blastocysts cultured in unsupplemented medium; this suggests that insulin increased the size of blastocysts and the rate of protein synthesis per unit size. The results indicate that pig blastocysts respond to physiological levels of insulin in similar fashion to those of mice and cattle, supporting the hypothesis that insulin may act as a general embryonic growth factor. Because of the cross reaction between the insulin receptor and the ligands, insulin and insulin-like growth factor 1 (IGF-1), the results also suggest that IGF-1, reported to be present in pig uterine fluid, could be involved in this stimulation in utero.