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1.
Clin Exp Immunol ; 200(3): 242-249, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32115683

RESUMO

Chronic spontaneous urticaria (CSU) pathogenesis shows a complex and still unclear interplay between immunoglobulin (Ig)G- and IgE-mediated autoimmunity, leading to mast cell and basophil degranulation and wheal formation. The objective of this study was to evaluate at the same time IgE- and IgG-reactivity to well recognized and recently reported autoantigens in CSU patients, and to assess the effects of such reactivity on response to the anti-IgE monoclonal antibody omalizumab. Twenty CSU patients underwent omalizumab treatment. Urticaria activity score 7 (UAS7) was recorded at baseline and at different drug administration time-points for categorizing early-, late- or non-responders. At baseline, sera from the 20 patients and from 20 controls were tested for IgE and IgG autoantibodies to high- and low-affinity IgE receptors (FcεRI and FcεRII), tissue factor (TF) and thyroglobulin (TG) by immunoenzymatic methods. Antibody levels were compared with those of controls and analysed according to response. Eighteen patients were omalizumab responders (11 early and seven late), while two were non-responders. More than 50% of patients had contemporary IgE and IgG to at least to one of the four different autoantigens. Late responders showed higher levels of both anti-TF IgE and IgG than early responders (P = 0·011 and P = 0·035, respectively). Twenty-five per cent of patients had levels of anti-FcεRI IgE, exceeding the upper normal limit, suggesting that it could be a novel auto-allergen in CSU. In CSU, there is an autoimmune milieu characterized by the co-existence of IgE and IgG autoantibodies to the same antigen/allergen, particularly in late responders to omalizumab, possibly explaining the slower response.


Assuntos
Autoanticorpos , Autoantígenos , Urticária Crônica , Imunoglobulina E , Imunoglobulina G , Omalizumab/administração & dosagem , Adulto , Idoso , Idoso de 80 Anos ou mais , Autoanticorpos/sangue , Autoanticorpos/imunologia , Autoantígenos/sangue , Autoantígenos/imunologia , Urticária Crônica/sangue , Urticária Crônica/tratamento farmacológico , Urticária Crônica/imunologia , Feminino , Humanos , Imunoglobulina E/sangue , Imunoglobulina E/imunologia , Imunoglobulina G/sangue , Imunoglobulina G/imunologia , Lectinas Tipo C/sangue , Lectinas Tipo C/imunologia , Masculino , Pessoa de Meia-Idade , Receptores de IgE/sangue , Receptores de IgE/imunologia , Tromboplastina/imunologia , Tromboplastina/metabolismo , Tireoglobulina/sangue , Tireoglobulina/imunologia
2.
Haemophilia ; 23(4): e348-e353, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28594432

RESUMO

INTRODUCTION: Patients with haemophilia B who develop factor IX (FIX) neutralizing antibodies (inhibitors) after FIX infusion are at high risk of hypersensitivity reactions upon FIX re-exposure, but the underlying mechanisms are incompletely understood. AIM: To investigate biomechanisms of FIX hypersensitivity. METHODS: A cellular antigen stimulation test (CAST) was employed to evaluate leukotriene C4 (LTC4) release from basophils stimulated by FIX in three treated children with haemophilia B, one of whom developed FIX inhibitor and experienced anaphylaxis following FIX re-exposure. Anti-FIX IgE and IgG antibodies and markers of complement activation (C5b9, C3d and iC3b) were measured in plasma, the last also after FIX infusion. Ten healthy children served as controls. RESULTS: The patient who developed anti-FIX inhibitors and anaphylaxis had a nonsense mutation in FIX gene (p.Arg298Stop) and, compared to controls, had higher plasma levels of specific anti-FIX IgE (2.285 vs 0.084 OD492 nm ), with marked LTC4 release from his FIX-stimulated basophils (519.8 vs 39.9 pg/mL). Further, he had higher plasma levels of anti-FIX IgG of all the four subclasses (total IgG 1.180 vs 0.120 OD492 nm ) with FIX neutralizing activity (1.5 BU); mild complement activation occurred during FIX-induced anaphylaxis (C5b9 increased from 258.5 to 351.1 ng/mL). The same parameters were normal in the two patients who tolerated FIX infusion. CONCLUSION: In the patient with haemophilia B who experienced anaphylaxis after FIX, but not in the patients with haemophilia B who tolerated FIX, the CAST assay showed FIX-induced LTC4 release, which was associated with high plasma levels of specific anti-FIX IgE and IgG antibodies.


Assuntos
Anafilaxia/complicações , Anticorpos Neutralizantes/imunologia , Basófilos/imunologia , Ativação do Complemento , Fator IX/imunologia , Hemofilia B/imunologia , Imunoglobulina E/imunologia , Pré-Escolar , Hemofilia B/complicações , Humanos , Masculino
3.
J Eur Acad Dermatol Venereol ; 29(4): 813-7, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24650303

RESUMO

BACKGROUND: Bullous pemphigoid (BP) is an autoimmune blistering disease due to autoantibodies against two hemidesmosomal antigens, namely BP180 and BP230, and characterized by coagulation activation both at cutaneous and systemic levels. Skin-infiltrating eosinophils contribute to bulla formation and, upon activation, are supposed to initiate the coagulation cascade. OBJECTIVE: The aim of this study was to investigate whether the activation of eosinophils and coagulation are linked in BP. METHODS: We evaluated the correlation between eosinophil cationic protein (ECP) levels and concentrations of the prothrombotic markers F1 + 2 and D-dimer in blister fluid and blood samples of 30 BP patients. Thirty healthy subjects were used as normal controls. RESULTS: ECP, F1 + 2 and D-dimer plasma levels were significantly higher in BP patients than in normal subjects. A significant correlation was found between ECP plasma levels and blood eosinophil count (r = 0.54, P = 0.002). F1 + 2 plasma levels positively correlated with disease severity, expressed as the percentage of body surface area involved (r = 0.36, P = 0.048). A striking increase in ECP (288.8 ± 45.2 ng/mL), F1 + 2 (31 409.9 ± 2929.4 pmol/L) and D-dimer levels (342 798.3 ± 44 206 ng/mL) was found in blister fluid from BP patients. In blister fluid, ECP levels were significantly higher than in peripheral blood (P < 0.0001) and were positively correlated with the levels of both F1 + 2 (r = 0.4, P = 0.02) and D-dimer (r = 0.5, P = 0.0045). CONCLUSIONS: ECP levels are strikingly elevated in blister fluids from BP patients and correlate with markers of coagulation activation, supporting the view that eosinophils initiate the coagulation cascade at skin level.


Assuntos
Vesícula/metabolismo , Proteína Catiônica de Eosinófilo/sangue , Eosinófilos , Produtos de Degradação da Fibrina e do Fibrinogênio/metabolismo , Penfigoide Bolhoso/sangue , Fragmentos de Peptídeos/sangue , Idoso , Idoso de 80 Anos ou mais , Contagem de Células Sanguíneas , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Protrombina , Índice de Gravidade de Doença
4.
Artigo em Inglês | MEDLINE | ID: mdl-22533230

RESUMO

BACKGROUND: The pathophysiology and triggers of idiopathic nonhistaminergic angioedema are unclear. This study aimed to assess autoreactivity in recurrent idiopathic angioedema associated or not with wheals. METHODS: The study population comprised 19 patients with recurrent idiopathic nonhistaminergic angioedema without wheals, 38 patients with angioedema and chronic urticaria (CU), and 52 patients with CU without angioedema. Twenty healthy individuals served as controls. Autoreactivity was evaluated in vivo using the autologous serum skin test (ASST) and in vitro by measuring serum-induced basophil histamine release (BHR). RESULTS: ASST results were negative in all patients with idiopathic angioedema without wheals and in healthy controls and positive in 29 of the 38 patients with angioedema and CU (76.3%) and in 26 of the 52 patients with CU without angioedema (50%) (P < .0001 for both CU groups). BHR was negative in the healthy controls and positive in 2 of the 19 patients with idiopathic angioedema without wheals (10.5%), in 18 of the 38 patients with angioedema and CU (47.3%) (P < .0001), and in 11 of the 52 patients with CU without angioedema (21.1%) (P < .03). CONCLUSION: The different rates of autoreactivity observed in patients with idiopathic nonhistaminergic angioedema without wheals and in patients with CU either with or without angioedema suggest that these disorders have a different pathophysiology. The failure to detect circulating vasoactive factors and histamine-releasing autoantibodies explains why H1 antihistamines are scarcely effective in most patients with idiopathic angioedema without wheals. However, they represent the cornerstone of CU treatment.


Assuntos
Angioedema/imunologia , Autoanticorpos/imunologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Angioedema/sangue , Basófilos/imunologia , Estudos de Casos e Controles , Feminino , Liberação de Histamina/imunologia , Humanos , Masculino , Pessoa de Meia-Idade , Testes Cutâneos/métodos , Urticária/imunologia , Adulto Jovem
5.
Eur Ann Allergy Clin Immunol ; 44(5): 188-92, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23156066

RESUMO

Eotaxin is a potent agonist for CC chemokine receptor 3 that can attract eosinophils at sites of inflammation. Given the potential role of eosinophils in chronic spontaneous urticaria (CU), we measured serum eotaxin levels together with C-reactive protein in 100 CU patients who were characterized according to autologous serum skin test (ASST) and disease severity. Serum eotaxin concentration was significantly higher in CU patients (median 140.1 pg/ml, range 33.7-718.7 pg/ml) than in 45 healthy controls (median 108.9 pg/ml, range 45.5-409.4 pg/ml) (p = 0.032) Serum eotaxin concentration was not significantly different in ASST-positive and ASST-negative patients as well as in patients with different urticaria activity scores. However, eotaxin levels tended to be higher in patients with intense symptoms. In the 7 patients observed during CU exacerbation and during remission, eotaxin serum levels tended to decrease during remission, although statistical significance was not reached (median concentration decreased from 170.0 pg/ml to 123.8 pg/ml). CRP levels were not significantly different in CU patients and healthy subjects, although there was a trend towards higher levels in the former population. Furthermore, in the 7 patients observed during CU exacerbation and during remission, CRP levels decreased significantly during remission (median concentration dropped from 4.1 microg/ml to 0.7 microg/ml, p = 0.015). No significant correlation was found between eotaxin and CRP serum levels. These findings indicate that serum eotaxin levels are increased in CU patients, although they do not reflect strictly disease activity. A role for eotaxin in eosinophil attraction and activation in CU can be envisaged.


Assuntos
Quimiocina CCL11/sangue , Urticária/sangue , Adulto , Proteína C-Reativa/análise , Quimiocina CCL11/imunologia , Doença Crônica , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Masculino , Urticária/imunologia
6.
Clin Exp Allergy ; 40(6): 875-81, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20214668

RESUMO

BACKGROUND: Matrix metalloproteinase-9 (MMP-9) is an endopeptidase produced by many inflammatory cells that has been found in increased amounts in plasma from patients with chronic urticaria (CU). OBJECTIVE: To evaluate plasma levels of MMP-9 and its tissue inhibitor of metalloproteinase-1 (TIMP-1) in CU patients in relation with disease severity, C-reactive protein (CRP) and circulating histamine-releasing factors. METHODS: Fifty-two consecutive CU patients were included in the study and disease activity was graded from 0 to 3. Plasma MMP-9, TIMP-1 and CRP levels were measured by enzyme immunoassays. Circulating histamine-releasing factors were assessed using in vivo (autologous serum skin test) and in vitro (basophil histamine release) tests. Seven CU patients were studied both during active disease and during remission. Thirty healthy subjects were used as normal controls. RESULTS: Plasma levels of MMP-9, TIMP-1 and CRP were significantly higher in CU patients than in healthy controls (P=0.0001, 0.003 and 0.005, respectively) and a trend towards a higher MMP-9/TIMP-1 molar ratio was found (P=0.051). A significant correlation was found between plasma MMP-9 levels and urticaria severity score (r=0.48, P<0.0001). CRP levels correlated with MMP-9 levels (r=0.37, P=0.008) and CU severity score (r=0.52, P=0.0001), but not with TIMP-1 (r=0.13) concentrations. MMP-9, TIMP-1 and CRP plasma levels and MMP-9/TIMP-1 molar ratio did not differ in patients either with or without an evidence of circulating histamine-releasing factors. Seven patients evaluated during remission showed a significant reduction of MMP-9 and CRP plasma levels. CONCLUSION: Plasma levels of MMP-9 and its inhibitor TIMP-1 are increased in CU patients. MMP-9 levels are associated with disease severity and CRP levels, but not with skin reactivity to autologous serum and with circulating histamine-releasing factors. These findings suggest that in CU there is an ongoing inflammatory process independent of the presence of circulating histamine-releasing factors.


Assuntos
Proteína C-Reativa/metabolismo , Metaloproteinase 9 da Matriz/sangue , Urticária/fisiopatologia , Adulto , Biomarcadores Tumorais/sangue , Doença Crônica , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Masculino , Índice de Gravidade de Doença , Testes Cutâneos , Inibidor Tecidual de Metaloproteinase-1/sangue , Proteína Tumoral 1 Controlada por Tradução , Urticária/sangue
7.
Allergy ; 64(11): 1616-22, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19485983

RESUMO

BACKGROUND: Although chronic urticaria (CU) is often regarded as autoimmune in nature, only less than 50% of sera from CU patients contain histamine-releasing autoantibodies. This suggests that other factors may contribute to its pathogenesis. We evaluated the possible involvement of vascular endothelial growth factor (VEGF), one of the major mediators of vascular permeability, in CU. METHODS: Eighty consecutive adult patients with CU and 53 healthy subjects were studied. VEGF and prothrombin fragment F(1+2) were measured by enzyme immunoassays. Autologous plasma skin test (APST) was performed in CU patients and, in six of them, skin biopsy specimens were taken from wheals to evaluate the immunohistochemical expression of VEGF and eosinophil cationic protein (ECP). RESULTS: Plasma VEGF concentrations were higher in CU patients (8.00 +/- 0.90 pmol/l) than in controls (0.54 +/- 0.08 pmol/l) (P = 0.0001) and tended to parallel both the severity of CU and to correlate with F(1+2) levels. APST was positive in 85.1% of patients. VEGF concentration was significantly higher in APST-positive than in APST-negative patients (P = 0.0003). Immunohistochemically, all specimens from patients with CU showed a strong expression of VEGF (P = 0.002) that colocalized with ECP, a classic eosinophil marker. CONCLUSIONS: VEGF plasma levels are elevated in CU and parallel the disease severity. This supports a possible role of this molecule in CU pathophysiology. Eosinophils are the main cellular source of VEGF in CU lesional skin.


Assuntos
Eosinófilos/metabolismo , Pele/imunologia , Urticária/fisiopatologia , Fator A de Crescimento do Endotélio Vascular/sangue , Fator A de Crescimento do Endotélio Vascular/metabolismo , Adulto , Doença Crônica , Proteína Catiônica de Eosinófilo/metabolismo , Eosinófilos/imunologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pele/citologia , Pele/fisiopatologia , Urticária/imunologia , Urticária/metabolismo
8.
Eur Ann Allergy Clin Immunol ; 41(6): 181-6, 2009 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-20128232

RESUMO

BACKGROUND: There is some evidence suggesting that factors other than autoantibodies to FceRI or IgE and histamine released from mast cells may play a role in skin autoreactivity that characterizes many patients with chronic urticaria (CU) and, possibly, in the pathogenesis of this disease. OBJECTIVE: The effect of antihistamine treatment on autologous plasma skin test (APST) in patients with CU was assessed. METHODS: 24 patients with CU underwent autologous plasma skin test (APST) as well as SPT with histamine 10 mg/ml while taking antihistamines. In 6 cases the same tests had been carried out also before the start of antihistamine treatment. Plasma levels of D-dimer, prothrombin F 1+2 fragment, and vascular endothelial growth factor (VEGF) were measured in 21 patients. RESULTS: 21/24 (87%) patients showed a large flare on APST while taking antihistamines while the skin reaction to histamine 10 mg/ml was abolished or negligible. Little difference in the autologous plasma-induced flare was seen before and after the start of cetirizine therapy in 6 cases, whereas the drug exerted a marked effect on the histamine SPT as well as on the autologous plasma-induced wheal. The APST-induced flare was not associated with patients' response to antihistamine. Plasma levels of VEGF, prothrombin F 1+2 fragment, and D-dimer were increased in plasmas from 8, 9, and 2 patients, respectively. CONCLUSIONS: Factors other than histamine are probably involved in the flare following APST in CU; such factors might play a pathogenic role particularly in patients not responding to standard antihistamine treatments.


Assuntos
Antagonistas dos Receptores Histamínicos/uso terapêutico , Plasma/imunologia , Pele/imunologia , Urticária/imunologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Doença Crônica , Feminino , Humanos , Injeções Intradérmicas , Masculino , Pessoa de Meia-Idade , Testes Cutâneos , Fator A de Crescimento do Endotélio Vascular/sangue
9.
Respir Med ; 101(12): 2526-33, 2007 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-17884421

RESUMO

BACKGROUND: We have recently observed that skin reactivity to autologous serum injection is common in patients with non-allergic asthma. However, clinical significance of skin reactivity to autologous serum remains to be defined. OBJECTIVE: To evaluate the possible relation between skin reactivity to autologous serum and clinical and laboratory characteristics in a series of patients with non-allergic asthma. METHODS: Fifty-five patients with non-allergic asthma underwent in vivo autologous serum skin test (ASST) and in vitro basophil histamine release assay using basophils from a normal donor. Clinical and laboratory characteristics including peripheral blood eosinophilia, antinuclear antibodies and total IgE concentration were evaluated. As control, ASST was performed in 10 allergic asthmatic patients, 10 patients with allergic rhinitis and 10 normal subjects. RESULTS: ASST was positive in 29/55 non-allergic asthmatics (53%), whereas it was negative in all 30 control subjects (P<0.001). The sera of 6 out of 51 patients induced in vitro histamine release from autologous basophils. The sera from two patients induced histamine release from membrane IgE-stripped basophils. A significant predominance of female sex (83%) and a high incidence of antinuclear antibodies (ANA) positivity (55%) were found among ASST-positive patients. CONCLUSION: These findings indicate that ASST is positive in about half patients with non-allergic asthma and that a proportion of patients (16%) has functional evidence of circulating histamine-releasing factors. In addition, predominance of female sex and frequent ANA positivity are in line with an autoimmune basis of non-allergic asthma.


Assuntos
Asma/imunologia , Doenças Autoimunes/imunologia , Pele/imunologia , Adulto , Idoso , Anticorpos Antinucleares/sangue , Basófilos/imunologia , Estudos de Casos e Controles , Eosinofilia , Feminino , Liberação de Histamina , Humanos , Hipersensibilidade Imediata/imunologia , Imunoglobulina E/sangue , Testes Imunológicos , Masculino , Pessoa de Meia-Idade , Rinite Alérgica Sazonal/imunologia , Soro/imunologia , Testes Cutâneos , Transplante Autólogo
10.
Artigo em Inglês | MEDLINE | ID: mdl-17460952

RESUMO

We describe the case of a 30-year-old woman who reported several episodes of anaphylaxis with angioedema and relapsing urticaria. Some events were related to nonsteroidal anti-inflammatory drug intake and one episode followed alcohol ingestion, but in most cases no triggers could be identified. Specific immunoglobulin E determination was negative for food and drug allergens, C3 and C4 were in the normal range, C1 inhibitor was slightly reduced and serum tryptase was undetectable. In vivo autologous serum skin test and in vitro basophil histamine release assay were positive indicating the presence of circulating histamine-releasing factors. When oral tolerance tests were performed, only doxycycline was tolerated whereas levofloxacin, clarithromycin, nimesulide and tramadol caused mild urticaria. Premedication with cetirizine allowed the patient to tolerate levofloxacin, clarithromycin and nimesulide. The demonstration of circulating histamine-releasing factors in a patient with idiopathic anaphylaxis and multiple drug allergy syndrome provides a new mechanistic insight and might open the way to new therapeutic approaches.


Assuntos
Anafilaxia/imunologia , Biomarcadores Tumorais/sangue , Cetirizina/uso terapêutico , Hipersensibilidade a Drogas/prevenção & controle , Antagonistas não Sedativos dos Receptores H1 da Histamina/uso terapêutico , Testes Cutâneos/métodos , Adulto , Anafilaxia/sangue , Antibacterianos/imunologia , Hipersensibilidade a Drogas/sangue , Hipersensibilidade a Drogas/diagnóstico , Hipersensibilidade a Drogas/imunologia , Feminino , Humanos , Síndrome , Proteína Tumoral 1 Controlada por Tradução
11.
J Neuroimmunol ; 38(1-2): 155-61, 1992 May.
Artigo em Inglês | MEDLINE | ID: mdl-1315794

RESUMO

In this study we evaluated the release of some mediators of inflammatory reactions such as histamine (H), leukotriene B4 (LTB4), leukotriene C4 (LTC4) and prostaglandin D2 (PGD2) in the cerebrospinal fluid (CSF) of 15 patients with acquired immunodeficiency syndrome (AIDS), eight with opportunistic infections of the central nervous system (CNS) and seven without HIV-related neurological pathology, and of 25 HIV-negative control subjects with other neurological diseases. The cerebrospinal LTB4 level was increased in all the AIDS patients (mean 348 pg/ml); the control group revealed normal levels of LTB4 in the CSF (mean 63.2 pg/ml). The PGD2 level in the HIV-positive (mean 264 pg/ml) patients was higher than of the control subjects (mean 50 pg/ml), while low LTC4 levels were found both in the HIV-positive and control groups. We did not find any significant concentration of H in the CSF of either the HIV-positive or the control subjects. These findings may be due to the presence of chronic HIV infection or to the opportunistic infections of the CNS that so often occur in the latest stages of the disease.


Assuntos
Encefalopatias/etiologia , Soropositividade para HIV/líquido cefalorraquidiano , Histamina/líquido cefalorraquidiano , Leucotrienos/líquido cefalorraquidiano , Prostaglandina D2/líquido cefalorraquidiano , Criptococose/etiologia , Soropositividade para HIV/complicações , Humanos , Leucotrieno B4/líquido cefalorraquidiano , SRS-A/líquido cefalorraquidiano , Toxoplasmose/etiologia
12.
Artigo em Inglês | MEDLINE | ID: mdl-15736721

RESUMO

A case of chronic urticaria in a child 6 years old is described. The strong skin reactivity upon intradermal injection of autologous serum suggested an autoreactive pathogenesis; however, patient's serum was unable to induce histamine release from basophils in-vitro, indicating the presence of a histamine-releasing factor specific for mast cells, and possibly other than an anti-FcepsilonRI or anti-IgE antibody. Intradermal test with autologous serum may be useful in revealing the autoreactive nature of chronic urticaria and can avoid a frustrating search for other causes of the disease. In children presenting with chronic or recurrent urticaria the diagnostic workup should include the autologous serum skin test.


Assuntos
Urticária/etiologia , Criança , Doença Crônica , Humanos , Masculino , Testes Cutâneos , Urticária/imunologia
13.
Artigo em Inglês | MEDLINE | ID: mdl-15160444

RESUMO

OBJECTIVES: The aim of this study was to determine prevalence and risk factors for latex hypersensitivity among health care workers (HCW) of an Italian general hospital. METHODS: 1747 HCW of the Ospedale Maggiore Policlinico of Milan were asked to fill in a questionnaire regarding latex-related manifestations (LRM) and personal medical history, and latex-specific IgE were measured by RAST-Cap system. RESULTS: 672 out of 1747 HCW (38.4%) answered to the questionnaire. LRM were reported by 168 out of 672 HCW (25%). The most common manifestation was hand dermatitis and itching (86.3%), followed by urticaria (3.5%) and respiratory symptoms (2.9%). Among the HCW with LRM, 75 (44.6%) reported a personal history of atopy and 24 (14.3%) reported oral allergy syndrome. most commonly related to kiwi, tomato, peach and melon/watermelon. Latex-specific IgE were found in 62 out of 1747 HCW (3.6%). Among the subjects answering the questionnaire, latex-specific IgE positivity was associated with occurrence of LRM (most commonly allergic contact dermatitis) and a longer professional exposure. The risk of latex IgE sensitisation was four times higher in HCW reporting atopic manifestations than in HCW without atopic disorders. Prevalence of LRM and latex-specific IgE was significantly higher among workers of auxiliary staff than among other job categories. The highest latex-specific IgE levels were found in subjects with severe latex-related symptoms and a personal history of atopy. CONCLUSIONS: A high prevalence of LRM was found among the HCW of an Italian general hospital, although a true latex sensitisation was detected only in a minority of cases. Members of the auxiliary staff, who wear latex gloves for several hours a day, had an increased prevalence of LRM and latex sensitisation. Atopy was a major risk factor for LRM and latex-specific IgE response.


Assuntos
Pessoal de Saúde/estatística & dados numéricos , Hipersensibilidade ao Látex/diagnóstico , Hipersensibilidade ao Látex/epidemiologia , Adulto , Distribuição por Idade , Intervalos de Confiança , Estudos Transversais , Feminino , Hospitais , Humanos , Itália/epidemiologia , Masculino , Pessoa de Meia-Idade , Razão de Chances , Testes do Emplastro , Prevalência , Probabilidade , Fatores de Risco , Índice de Gravidade de Doença , Distribuição por Sexo , Estatísticas não Paramétricas , Inquéritos e Questionários
14.
Int J Clin Pharmacol Res ; 6(4): 309-14, 1986.
Artigo em Inglês | MEDLINE | ID: mdl-2875955

RESUMO

The kinetics of delorazepam (chlordesmethyldiazepam; CDDZ), and its major metabolite, lorazepam (LRZ) during multiple-dose therapy have been evaluated in two groups of patients with primary or secondary anxiety. The 12 patients in group 1 were 46.8 +/- less than 13.2 years while the eight in group 2 were significantly older (69.7 +/- 7.8 years). All patients were given 0.5 mg twice daily of CDDZ for 30 days. Concentrations of CDDZ and LRZ in multiple blood samples collected during the study were determined by electron-capture gas-liquid chromatography. The degree of anxiety was evaluated from the Hamilton rating scale for anxiety (HRSA). CDDZ and LRZ accumulated in plasma but the rate of accumulation of CDDZ was slower than expected from studies in young volunteers and the half-life values were significantly related to age. Steady-state levels of glucuronated LRZ were also lower in elderly patients. Data indicate that CDDZ is more slowly eliminated and less metabolized as age increases. While pre-treatment scores of HRSA were similar in the two groups, older patients improved significantly less than those of group 1 and had also an higher incidence of side-effects. CDDZ levels positively correlated with improvement in group 1 but not in group 2.


Assuntos
Envelhecimento/sangue , Ansiolíticos/sangue , Transtornos de Ansiedade/sangue , Benzodiazepinas , Diazepam/análogos & derivados , Nordazepam/análogos & derivados , Adulto , Idoso , Ansiolíticos/administração & dosagem , Ansiolíticos/efeitos adversos , Transtornos de Ansiedade/tratamento farmacológico , Feminino , Humanos , Cinética , Masculino , Pessoa de Meia-Idade , Nordazepam/administração & dosagem , Nordazepam/efeitos adversos , Nordazepam/sangue
15.
J Wildl Dis ; 26(4): 538-9, 1990 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-2250331

RESUMO

Two cases of dioctophymosis in wild little grisons (Galictis cuja) were found in Paraná State (southern Brazil). One female host was infected with eight nematodes and a male harbored a single parasite. This represents the first report of the giant kidney nematode (Dioctophyme renale) in the little grison.


Assuntos
Carnívoros/parasitologia , Nefropatias/veterinária , Infecções por Nematoides/veterinária , Animais , Brasil , Feminino , Rim/parasitologia , Nefropatias/parasitologia , Masculino , Infecções por Nematoides/parasitologia
16.
Clin Ter ; 131(3): 173-6, 1989 Nov 15.
Artigo em Italiano | MEDLINE | ID: mdl-2575479

RESUMO

The object of the above study was to evaluate the tolerability and possible therapeutic use of MgNAAGA (Mg salt of N-acetyl-aspartyl-glutamic acid) in patients with allergic rhinitis. Out of 22 subjects with respiratory allergy, 12 were treated for 15 days with MgNAAGA and 10 with DSCG for the same length of time. During this period, patients kept a diary in which they recorded the following symptoms: rhinorrhea, itching, sneezing and nasal obstruction. The trial drug was found to be well tolerated and not to give rise to relevant side effects. The therapeutic efficacy was similar to that of DSCG.


Assuntos
Dipeptídeos/uso terapêutico , Antagonistas dos Receptores Histamínicos H1/uso terapêutico , Rinite Alérgica Perene/tratamento farmacológico , Rinite Alérgica Sazonal/tratamento farmacológico , Administração Intranasal , Adolescente , Adulto , Cromolina Sódica/uso terapêutico , Dipeptídeos/administração & dosagem , Avaliação de Medicamentos , Antagonistas dos Receptores Histamínicos H1/administração & dosagem , Humanos
17.
Allerg Immunol (Paris) ; 20(2): 45-7, 1988 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-2456082

RESUMO

Liberation of histamine and LTC4 by cord-blood basophils was measured in the newborn from healthy and atopic parents. The cord-blood basophils of the second classification produced more histamine than those from the first, after challenge with anti-IgE. Thus, a newborn from atopic parent(s) probably carries more fixed IgE on the basophils than a newborn from healthy parents and so the capacity of cord-blood basophils to liberate mediators may be a good marker of allergy.


Assuntos
Basófilos/imunologia , Sangue Fetal/imunologia , Liberação de Histamina , Hipersensibilidade/diagnóstico , Adulto , Basófilos/metabolismo , Humanos , Hipersensibilidade/sangue , Hipersensibilidade/imunologia , Imunoglobulina E/análise , Recém-Nascido , SRS-A/biossíntese
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