RESUMO
The technique of parallel automated synthesis of oligodeoxynucleotides bearing various local thiophosphoryl internucleotide bonds was optimized using assembling in the standby mode and creation of special program blocks. The selected conditions of Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI TOF MS) provided an increase in the method sensitivity (up to 1-10 fmol of oligonucleotide in sample) and registration of reliable spectra of oligodeoxynucleotide thiophosphoryl analogues. This enables to reliably prove the presence of the specified number of thiophosphoryl bonds within synthetic sequences. A series of oligodeoxynucleotides, thioanalogues of d(GGTTGGTGTGGTTGG), a known G-quadruplex antithrombin aptamer, were obtained.
Assuntos
Aptâmeros de Nucleotídeos/síntese química , Fibrinolíticos/síntese química , Oligonucleotídeos/síntese química , Fosfatos/síntese química , Aptâmeros de Nucleotídeos/química , Fibrinolíticos/química , Oligonucleotídeos/química , Fosfatos/química , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por MatrizRESUMO
The characteristics of the functional activity of phagocyte blood cells in patients with destructive pulmonary tuberculosis caused by medicinal sensitive and medicinal resistant infective agents were studied. In the process of pulmonary tuberculosis, irrespective of the medicinal sensitivity of infective agents before and during treatment, a decrease in the phagocytic activity of neutrophil granulocytes and the level of the expression of Fcgamma- and C3b-receptors on monocytes with a simultaneous increase in the spontaneous production of oxygen metabolites in neutophils and a rise in the adsorptive capacity of monocytic cells were observed.
Assuntos
Neutrófilos/imunologia , Tuberculose Pulmonar/imunologia , Adulto , Antituberculosos/uso terapêutico , Células Sanguíneas , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Monócitos/metabolismo , Neutrófilos/metabolismo , Oxigênio/metabolismo , Fagocitose , Receptores de Complemento 3b/metabolismo , Receptores de IgG/metabolismo , Tuberculose Pulmonar/tratamento farmacológicoRESUMO
The cytogenic status of peripheral lymphocytes was studied in 67 patients with drug-sensitive pulmonary tuberculosis. The latter was found to be attended by cytogenetic disorganization whose signs were present before and 2 months of intensive chemotherapy. In pulmonary tuberculosis, structural disorganization in the lymphocytic chromosomal apparatus was characterized by the elevated levels of cells and with changes in the structure and number of chromosomes. They are most pronounced in infiltrative and disseminated tuberculosis than in caseous pneumonia, as well as 2 months after rather than before chemotherapy. Patients with infiltrative pulmonary tuberculosis had also decreased lymphocytic activity of DNA reparation, followed by its normalization after a course of chemotherapy.
Assuntos
Antituberculosos/uso terapêutico , Núcleo Celular/genética , DNA/análise , Linfócitos/ultraestrutura , Tuberculose Pulmonar/sangue , Adulto , Técnicas de Cultura de Células , Núcleo Celular/efeitos dos fármacos , Núcleo Celular/ultraestrutura , DNA/efeitos dos fármacos , Seguimentos , Marcadores Genéticos , Humanos , Linfócitos/efeitos dos fármacos , Masculino , Pessoa de Meia-Idade , Tuberculose Pulmonar/tratamento farmacológico , Tuberculose Pulmonar/genéticaRESUMO
The paper presents the results of a study of spontaneous and stimulated production of TNF-alpha, INF-gamma, INF-alpha, IL-2 by peripheral mononuclear leukocytes in patients with disseminated destructive drug-resistant pulmonary tuberculosis. It also shows marked changes in the levels the studied cytokines in relation to the type of a stimulant (lipopolysaccharide, phytohemagglutinin, tuberculin) and during antituberculous chemotherapy.