Phylogenomic analysis of a highly virulent Escherichia coli ST83 lineage with potential animal-human transmission.

The presence of specific virulence features conditions severe forms of urinary tract disease, but the frequency and distribution of these highly virulent extraintestinal pathogenic Escherichia coli strains in animals and humans is unclear. We used whole genome sequencing, comparative genomics, histological and clinical data to characterize the genetic basis for pathogenesis and origin of E. coli Ec_151217, a strain (B2, ST83, O83:H5:K5) that caused an extremely aggressive upper urinary tract infection (UTI) in a cat. We show that Ec_151217 and 52% of other highly related ST83 genomes (O6 and O83) identified from different animal species and human infections carry two copies of the hemolysin A operon, though this duplication is infrequent (~1%) among closed ExPEC genomes from multiple sources. Our data enlarges the list of E. coli genetic backgrounds carrying hlyA operon duplication which is potentially involved in severity of UTI, and demonstrates that it seems to occur infrequently amongst ExPEC. Its identification in E. coli lineages (diverse ST83 serotypes) of potential animal-human transmission is of concern and anticipates the need to screen larger collections.

Typicality Assessment of Onions (Allium cepa) from Different Geographical Regions Based on the Volatile Signature and Chemometric Tools.

Onion (Allium cepa L.) is one of the main agricultural commodities produced and consumed around the world. In the present work, for the first time, the volatile signature of onions from different geographical regions of Madeira Island (Caniço, Santa Cruz, Ribeira Brava, and Porto Moniz) was tested with headspace solid-phase microextraction (HS-SPME/GC-qMS) and chemometric tools, showing that the volatile signature was affected by the geographical region of cultivation. Sulfur compounds, furanic compounds, and aldehydes are the most dominant chemical groups. Some of the identified volatile organic metabolites (VOMs) were detected only in onions cultivated in specific regions; 17 VOMs were only identified in onions cultivated at Caniço, eight in Porto Moniz, two in Santa Cruz, two in Ribeira Brava, while 12 VOMs are common to all samples from the four regions. Moreover, some VOMs belonging to sulfur compounds (dipropyl disulfide, 3-(acetylthio)-2-methylfuran), furanic compounds (dimethylmethoxyfuranone, ethyl furanone, acetyloxy-dimethylfuranone), and lactones (whiskey lactone isomer), could be applied as potential geographical markers of onions, providing a useful tool to authenticate onions by farming regions where the influence of latitude seems to be an important factor for yielding the chemical profile and may contribute to geographical protection of food and simultaneously benefiting both consumers and farmers.

Reestruturação das atividades de planejamento sexual e reprodutivo desenvolvido na USF Dom Avelar

O cuidado no que se refere aos direitos sexuais e reprodutivos na Atenção Primária à Saúde é fundamental, já que ela é considerada a porta de entrada aos serviços de saúde. Este plano de ação apresenta o objetivo de promover educação sexual e planejamento reprodutivo na USF Dom Avelar. O plano prevê busca ativa da população alvo através de cadastro prévio, catalogação da mesma por meio de uma planilha unificada, convocação por telefone ou visita de agentes comunitários de saúde para reavaliação médica, com reajuste da terapêutica e encaminhamento para serviços de referência. A identificação de pacientes assistidas na USF Dom Avelar em uso de terapia contraceptiva hormonal de forma errônea fez com que fossem revistas as estratégias contraceptivas disponibilizadas aos usuários, provendo, dessa forma, um cuidado individualizado e promovendo a saúde sexual adequada. Espera-se, com o plano, melhorias na saúde sexual e reprodutiva da população assistida a médio e longo prazo.