RESUMO
Acanthamoeba spp. are free-living amoebae that are ubiquitously distributed in the environment and can cause encephalomyelitis in animals and humans. The factors that contribute to Acanthamoeba infections include parasite biology, genetic diversity, environmental spread, and host susceptibility. The aim of the present study was to characterize isolates of Acanthamoeba from the nasal mucosa and cutaneous lesions of dogs in order to access the occurence and pathogenicity of these organisms in this animal group. We studied 13 isolates of Acanthamoeba confirmed by polymerase chain reaction. They were sequenced, the genotype was determined, and their potential of pathogenicity was evaluated.
Assuntos
Acanthamoeba/classificação , Acanthamoeba/isolamento & purificação , Amebíase/veterinária , Doenças do Cão/parasitologia , Mucosa Nasal/parasitologia , Ferimentos e Lesões/parasitologia , Acanthamoeba/genética , Acanthamoeba/patogenicidade , Amebíase/parasitologia , Animais , DNA de Protozoário/química , DNA de Protozoário/genética , Cães , Genótipo , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Análise de Sequência de DNA , VirulênciaRESUMO
Cattle Fasciola hepatica egg-viability was studied in closed system of anaerobic biodigestion. Two thirds of a biodigestor were filled with liquefied manure from eight Hosltein cows, naturally infected. For 10 consecutive weeks of observation, the biodigestor internal temperature ranged from 24ºC to 28ºC, and external temperature from 26ºC to 33ºC. All the effluent samples showed a constant pH of 7. The samples of the effluent were weekly collected, in a total of 10 and submitted to techniques to detect the presence and viability of the trematode eggs. It was detected a 42-day egg viability. Therefore, liquefied cattle manure containing Fasciola hepatica eggs must remain under anaerobic biodigestion for at least 42 days before its safe return to the environment.