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1.
Cell Mol Life Sci ; 79(2): 129, 2022 Feb 09.
Artigo em Inglês | MEDLINE | ID: mdl-35141765

RESUMO

Among all reactive oxygen species (ROS), hydrogen peroxide (H2O2) takes a central role in regulating plant development and responses to the environment. The diverse role of H2O2 is achieved through its compartmentalized synthesis, temporal control exerted by the antioxidant machinery, and ability to oxidize specific residues of target proteins. Here, we examine the role of H2O2 in stress acclimation beyond the well-studied transcriptional reprogramming, modulation of plant hormonal networks and long-distance signalling waves by highlighting its global impact on the transcriptional regulation and translational machinery.


Assuntos
Peróxido de Hidrogênio/farmacologia , Desenvolvimento Vegetal/efeitos dos fármacos , Plantas , Espécies Reativas de Oxigênio/metabolismo , Proteínas de Plantas/metabolismo , Plantas/efeitos dos fármacos , Plantas/metabolismo
2.
PLoS One ; 15(12): e0242545, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33259527

RESUMO

Heavy losses by dark leaf spot disease in oilseed Brassica have incited research towards identifying sources of genetic tolerance against causal pathogen, Alternaria brassicicola. Several morpho-molecular parameters were evaluated to test the performance of field mustard and rapeseed genotypes under artificial inoculation with this pathogen. During Brassica-Alternaria interaction, physio-biochemical defense response was witnessed in tolerant genotypes. Two tolerant genotypes (one for field mustard and one for rapeseed), i.e., EC250407 and EC1494 were identified. However, necrotic lesions were more prominent in susceptible genotypes with minimum chlorophyll (chlorophyll a, chlorophyll b and total chlorophyll) and carotenoids contents. Contrary to photosynthetic pigments, increase in total soluble protein (TSP) contents was observed with disease progression in susceptible genotypes. Tolerant genotypes of field mustard and rapeseed displayed remarkable increase in the activities of redox enzyme in infected leaves with least yield loss (6.47% and 5.74%) and disease severity index (DSI) of 2.9 and 2.1, respectively. However, yield/plant showed close association with other morpho-yield parameters, photosynthetic pigments and redox enzymes (superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD)) activities except silique length and TSP. Based on the results of morpho-biochemical analyses, redox enzymes and morphological parameters; their interplay is proposed to determine the tolerance outcome of the Brassica-A. brassicicola interaction.


Assuntos
Alternaria/patogenicidade , Brassica napus/genética , Brassica rapa/genética , Brassicaceae/genética , Antioxidantes/metabolismo , Brassica napus/crescimento & desenvolvimento , Brassicaceae/crescimento & desenvolvimento , Brassicaceae/parasitologia , Carotenoides , Catalase/genética , Clorofila/genética , Clorofila A/genética , Resistência à Doença/genética , Genótipo , Oxirredução , Fotossíntese , Doenças das Plantas/genética , Doenças das Plantas/parasitologia , Superóxido Dismutase/genética
3.
ACS Omega ; 5(41): 26845-26854, 2020 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-33111010

RESUMO

Recently, a new class of two-dimensional (2D) materials, called MXene, consisting of layers of transition-metal carbides and nitrides/carbonitrides has been introduced. MXene, a multifunctional material with hydrophilic nature and excellent electrical conductivity and chemical stabilities, can be applied in diverse research areas such as energy harvesting and its storage, water purification, thermal dissipation, and gas sensing. To achieve the best quality of MXene, optimization of some important synthetic parameters is highly required such as an optimized etchant concentration to remove an "A" element from the MAX phase and sonication time for the efficient exfoliation of MXene flakes. Besides, there is a need to disclose that particular solvent through which intercalation can easily be achieved. In this work, we optimized the abovementioned critical parameters for the synthesis of good-quality MXene. Our results clearly explain the variations in the quality of MXene under applied etchant concentrations, solvents for better intercalation, and optimization of sonication time for better exfoliation. The obtained results suggest that 30% HF as an etchant, dimethyl sulfoxide (DMSO) as a solvent, and 135 min as the sonication time are effective parameters for the synthesis of good-quality MXene. We expect that this report will be helpful for the young research community to synthesize good-quality MXene with the required properties.

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