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1.
Klin Lab Diagn ; 65(9): 541-546, 2020 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-33245638

RESUMO

The study includes anthropometry of 172 young male, obtained data on the length and body mass, measured the transverse diameters of the shoulders and pelvis, various body types was identified by the J.M. Tanner sexual dimorphism index (andromorphic, mesomorphic, gynecomorphic). The chemiluminescent and bioluminescent study of saliva and blood was conducted in the examined young male. We studied the indicators of the antioxidant defense system under the influence of stress. The antioxidant status of saliva was determined using the H2O2-luminol-dependent chemiluminescence method. Data on the activity of NAD (P) -dependent dehydrogenases in blood lymphocytes was obtained from a bioluminescent method of research. Young male of andromorphic body type had large overall and transverse body sizes. Indicators of antioxidant protection of saliva and blood in men of adolescence, the body type of the sexual dimorphism index J.M. Tanner was different. The persons of the andromorphic body type differed in terms of chemiluminescence in comparison with the young male of gynecomorphic body type. The results of bioluminescent blood tests suggest a violation of the catabolic and anabolic processes of carbohydrate and fat metabolism in young men of mesomorphic and gynecomorphic body types. Indicators of the system of antioxidant protection of saliva and blood reflect the sexual characteristics of the body of young male and can be used as additional criteria for diagnosing sex inversion and assessing the risk of developing socially attributed diseases.


Assuntos
Peróxido de Hidrogênio , Saliva , Adolescente , Antropometria , Antioxidantes , Humanos , Masculino , Caracteres Sexuais
2.
Bull Exp Biol Med ; 160(4): 444-7, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26902353

RESUMO

In vitro experiments showed that heparin adsorbed on activated charcoal can bind antibodies raised against native and single-stranded DNA in a diluted sera pool with a high level of these DNA. Thus, heparin used as anticoagulant during hemosorption procedure can demonstrate supplementary therapeutic activity resulting from its interaction with various agents involved in acute and chronic inflammatory reactions such as DNA- and RNA-binding substances, proinflammatory cytokines, complement components, growth factors, etc. Research and development of heparin-containing carbonic adsorbents for the therapy of numerous inflammatory and autoimmune diseases seems to be a promising avenue in hematology.


Assuntos
Anticorpos Antinucleares/imunologia , Carvão Vegetal/metabolismo , DNA de Cadeia Simples/imunologia , Heparina/metabolismo , Adsorção/fisiologia , Animais , Anticorpos Antinucleares/uso terapêutico , Anticoagulantes/uso terapêutico , Doenças Autoimunes/terapia , Carvão Vegetal/uso terapêutico , Heparina/uso terapêutico , Coelhos
3.
Exp Oncol ; 43(4): 359-364, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34967551

RESUMO

AIM: To evaluate the influence of dispersed fibrous carbon sorbent (DFCS) on malignant fungating wounds (MFWs) in Guerin's carcinoma-bearing rats with an assessment of wound bacterial microflora. MATERIALS AND METHODS: The study was performed on female Wistar rats inoculated subcutaneously with Guerin T8 carcinoma into the upper interscapular region. On day 20, the tumors were infected by injecting the suspension of S. aureus 8325-4. After 24 h, gauze dressings were applied daily to the wounds in the control group and DFCS in the experimental one. The state of the wounds was assessed by visual control with photo monitoring, odor control on a verbal rating scale and microbiological analysis of qualitative and quantitative characteristics of wound microbiota. RESULTS: On the 3rd day after the start of dressings, the number of S. aureus of 8325-4 strain was significantly higher in the wounds of rats in control group than experimental one (5.14 ± 0.27 vs 3.43 ± 0.33 lg CFU/ml). The total number of Staphylococci in the DFCS-treated wounds did not differ statistically from the number of S. aureus 8325-4 strain whereas in control ones were higher by an order of magnitude. On the 10th day the total number of Staphylococci and S. aureus 8325-4 in particular, remained consistently high in the wounds of the control rats, while was almost five orders of magnitude lower and represented mainly by S. aureus 8325-4 in the experimental ones. The total number of aerobic and optionally anaerobic microorganisms was significantly lower as well. A greater variety of microorganisms in the gauze-covered wounds, as well as an their increased number were accompanied by enhancement of the wound odor from "noticeable" to "strong". There was an appearance of a "barely noticeable" odor in only one animal from the experimental group. CONCLUSION: The study has demonstrated the ability of DFCS to control substantially the bacterial microflora as well as malodor of MWFs in vivo. The results obtained can contribute to solving the problem of improving the quality of palliative care for patients with malignant and other chronic wounds.


Assuntos
Neoplasias , Staphylococcus aureus , Animais , Carbono , Feminino , Humanos , Neoplasias/complicações , Ratos , Ratos Wistar
4.
Exp Oncol ; 43(1): 21-25, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33785723

RESUMO

AIM: To evaluate the changes of some biochemical blood plasma parameters and morphological structure of the internal organs of rats with transplanted doxorubicin (DOX)-sensitive (Walker 256) and doxorubicin-resistant (Walker 256/DOX) strains of Walker 256 carcinosarcoma. MATERIALS AND METHODS: The study was performed on female Wistar rats with transplanted Walker 256 or Walker 256/DOX and intact animals (control). On the 9th day after transplantation of tumor cells, a comparative analysis of some blood plasma biochemical parameters and morphological examination of the liver, kidneys, myocardium and spleen of rats was carried out. RESULTS: Walker 256 growth, in comparison with Walker 256/DOX, is accompanied by more pronounced systemic effect on tumor-bearing rats. Uric acid concentration in the blood plasma of Walker 256 bearing rats was significantly (by 15.5%) higher than in Walker 256/DOX bearing rats. Aspartate aminotransferase activity in the Walker 256 group was significantly (by 107.2%) higher than in Walker 256/DOX group, but alanine aminotransferase activity was 58.5% lower. 56.7% decrease of alkaline phosphatase in rats with Walker 256, and 21% increase of this index in rats with Walker 256/DOX were observed. The growth of Walker 256 carcinosarcoma led to greater structural damage of the liver, kidneys and spleen in experimental animals compared with Walker 256/DOX strain. CONCLUSION: Tumor growth in rats with Walker 256/DOX leads to less pronounced changes in the biochemical parameters of rat blood plasma and morphological structure of internal organs compared with wild-type carcinosarcoma.


Assuntos
Carcinoma 256 de Walker/sangue , Carcinoma 256 de Walker/patologia , Resistencia a Medicamentos Antineoplásicos/fisiologia , Animais , Feminino , Ratos , Ratos Wistar
5.
Exp Oncol ; 42(1): 40-45, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-32231185

RESUMO

AIM: To assess oxidative stress and structural changes of the serum albumin in rats with transplanted Walker-256 carcinosarcoma (W256) strains with varying sensitivity to doxorubicin (Dox). MATERIALS AND METHODS: The study was performed on female Wistar rats with transplanted W256. On the 9th day after tumor cell transplantation an analysis of peripheral blood, oxidative stress parameters, and structural changes of serum albumin of experimental animals was performed. RESULTS: On the 9th day after W256 transplantation a significant increase in the leukocyte counts was observed in the groups of animals with the Dox-resistant and parental (Dox-sensitive) W256 tumors compared with the group of the intact animals: up to 14.24 ± 1.92 â€¢ 103/µl and 9.78 ± 1.03 â€¢ 103/µl, vs 8.92 ± 1.04 â€¢ 103/µl, respectively, due to the increase of granulocyte and monocyte counts. The number of lymphocytes was within the normal range. The level of hemoglobin and the erythrocyte counts were also within normal limits, but hematocrit in both groups of animals with tumors somewhat increased against the background of 1.2-fold elevation of the mean erythrocyte volume. In the group of rats with Dox-resistant W256, there was observed a decrease in the plateletcrit by almost 22% and thrombocyte counts - by 28%. Analysis of oxidative stress indices revealed a significant increase in the level of reactive oxygen species, 2-fold increase of malonic dialdehyde level and the degree of oxidative damage of blood plasma proteins, as well as a decrease in the activity of catalase in hemolysates (by 12-15%) in both groups of tumor-bearing rats. With the use of differential scanning calorimetry, UV and fluorescence spectroscopy we have revealed anomalous conformational changes of albumin caused by tumor development: structural rearrangements in the region of its first drug binding site located in the IIA domain, separation of globular parts of albumin molecule, and partial "opening" in a protein molecular three-domain structure resulting a loss of its thermal resistance. CONCLUSION: The development of transplanted Walker-256 carcinosarcoma, especially its Dox-resistant variant, results in severe metabolic intoxication reflected in alteration of hematological parameters, and indices of oxidative stress, as well as architectonic changes of serum albumin.


Assuntos
Antibióticos Antineoplásicos/farmacologia , Carcinoma 256 de Walker/sangue , Doxorrubicina/farmacologia , Resistencia a Medicamentos Antineoplásicos , Estresse Oxidativo , Espécies Reativas de Oxigênio/metabolismo , Albumina Sérica/química , Animais , Carcinoma 256 de Walker/metabolismo , Carcinoma 256 de Walker/patologia , Feminino , Transplante de Neoplasias , Estresse Oxidativo/efeitos dos fármacos , Conformação Proteica , Ratos Wistar
6.
Exp Oncol ; 41(3): 254-257, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31569931

RESUMO

The potential of one of the adsorption methods, enterosorption (ES), using the new generation of carbon adsorbents to correct the negative manifestations of tumor-host interaction in the framework of paraneoplastic syndrome (PNS) as well as systemic toxicity of chemo- and radiation therapy, is discussed. The ES influence on the development of PNS was demonstrated in C57/BL6 mice with transplanted Lewis lung carcinoma. Two-week administration of carbon enterosorbents resulted in a significant suppression of metastasis and correction of tumor-related anemia, activation of granulocytic line in the bone marrow with nearly 3-fold enhancement of its mitotic activity. ES exerted a positive influence on the structural-morphologic indexes and regenerative potential of kidneys and liver, mitigated manifestations of oxidative stress, decreased the level of endogenous intoxication, increased resistance of erythrocyte membranes and decreased ligand loading of blood plasma transport proteins. The effect of ES on anticancer activity and toxic reactions of cisplatin (CP) was evaluated in Guerin carcinoma-bearing rats. ES reduced significantly creatinine and other kidney biochemical indexes elevated in the blood plasma of rats after CP treatment. ES attenuated dystrophic changes in the histological structure of internal organs (kidney, liver, spleen), caused by tumor growth and significantly aggravated under the influence of CP. Such changes were specially traced in the kidneys and well reflect the nephroprotective potential of ES. In rats irradiated with X-ray in sublethal dose, highly activated granulated carbonic enterosorbents facilitated the restoration of white blood cells and lymphocyte count. The results obtained confirm the insights of academician R.E. Kavetsky predicting the future of adsorptive detoxification with activated carbons in the treatment of cancer patients.


Assuntos
Enteroadsorção , Neoplasias/terapia , Animais , Protocolos de Quimioterapia Combinada Antineoplásica/efeitos adversos , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Terapia Combinada/efeitos adversos , Terapia Combinada/métodos , Modelos Animais de Doenças , Enteroadsorção/efeitos adversos , Enteroadsorção/métodos , Humanos , Masculino , Camundongos , Neoplasias/complicações , Neoplasias/diagnóstico , Síndromes Paraneoplásicas/terapia , Resultado do Tratamento
7.
Exp Oncol ; 38(3): 172-5, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27685524

RESUMO

UNLABELLED: Today due to improvements in cancer treatment there is an increasing number of long-term cancer survivors, many of whom suffer from infertility caused by malignancy itself and chemo- or radiotherapy. Also, anticancer therapy may cause myelosuppression. Presently granulocyte colony stimulating factor (G-CSF) is used for prevention and treatment of myelosuppression. Another treatment option used to decrease intoxication and ameliorate side effects of cancer therapy is sorption technology. The aim of our investigation was to study the efficiency of combined use of enterosorption and G-CSF to decrease gonadal toxicity of chemotherapy. MATERIALS AND METHODS: Melphalan (L-PAM) injected i.v. at a single dose of 4 mg/kg to white inbred rats was used as gonadotoxic and myelosuppressing agent. Carbon enterosorbent C2 was administered by intragastric route as a suspension in saline at a dose of 5 ml per 1 kg of rats' body weight (or 900 mg/kg of the dry mass of enterosorbent) daily for 3 days before and for 7 days after L-PAM injection. G-CSF was injected once a day for 4 days starting from the next day after L-PAM administration at a dose of 50 µg/kg. Histological preparations of testicular tissues were examined by light microscopy. RESULTS: Our findings have shown that melphalan caused marked damage of testicular tissues and seminiferous, especially spermatogenic epithelium. The most expressed protection of the histological structure of testes was observed when enterosorbent and G-CSF were used in combination. CONCLUSION: Gonadal toxicity of chemotherapy could be efficiently decreased by the combined use of enterosorption and G-CSF.


Assuntos
Antineoplásicos Alquilantes/toxicidade , Enteroadsorção , Fator Estimulador de Colônias de Granulócitos/uso terapêutico , Melfalan/toxicidade , Agonistas Mieloablativos/toxicidade , Testículo/efeitos dos fármacos , Testículo/patologia , Animais , Carbono/uso terapêutico , Enteroadsorção/métodos , Masculino , Neoplasias/tratamento farmacológico , Substâncias Protetoras/uso terapêutico , Ratos
8.
Exp Oncol ; 37(2): 135-8, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26112942

RESUMO

UNLABELLED: Hematotoxicity and its complication are the prominent limiting factors for rational treatment of malignancies. Granulocyte colony-stimulating factor (G-CSF) is used to increase granulocyte production. It has been shown previously that enterosorption causes prominent myeloprotective activity also. Still, no trial was performed to combine both of them. AIM: To study the influence of combination of enterosorption and pharmaceutical analogue of naturally occurring G-CSF (filgrastim) on bone marrow protection and the growth of grafted tumor in a case of injection of melphalan (Mel). MATERIALS AND METHODS: Mel injections were used for promotion of bone marrow suppression in rats. Carbon granulated enterosorbent C2 (IEPOR) was used for providing of enteral sorption detoxifying therapy. Filgrastim was used to increase white blood cells (WBC) count. RESULTS: The simultaneous usage of enterosorption and filgrastim had maximum effectiveness for restoring of all types of blood cells. WBC count was higher by 138.3% compared with the Mel group. The increase of platelets count by 98.5% was also observed. In the group (Mel + C2 + filgrastim) the absolute neutrophils count was twofold higher, in comparison with rats of Mel group. CONCLUSION: Simultaneous administration of G-CSF-analogue and carbonic enterosorbent C2 is a perspective approach for bone marrow protection, when the cytostatic drug melphalan is used. Such combination demonstrates prominent positive impact on restoring of all types of blood cells and had no influence on the antitumor efficacy.


Assuntos
Antineoplásicos Alquilantes/efeitos adversos , Medula Óssea/efeitos dos fármacos , Carbono/farmacologia , Fator Estimulador de Colônias de Granulócitos/uso terapêutico , Melfalan/efeitos adversos , Neutropenia/prevenção & controle , Animais , Antineoplásicos Alquilantes/uso terapêutico , Medula Óssea/patologia , Enteroadsorção , Feminino , Fator Estimulador de Colônias de Granulócitos/farmacologia , Hematopoese/efeitos dos fármacos , Masculino , Melfalan/uso terapêutico , Transplante de Neoplasias , Neoplasias Experimentais/tratamento farmacológico , Neutropenia/induzido quimicamente , Ratos Wistar
9.
Exp Oncol ; 37(4): 255-61, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26710837

RESUMO

AIM: To study the correcting effects of microgranulated HSGD enterosorbent on hematological, morphological and biochemical indices of paraneoplastic syndrome in mice with highly angiogenic variant of Lewis lung carcinoma LLC/R9. METHODS: The study was performed on male С57/ВL6 mice with transplanted LLC/R9. Enterosorbent HSGD was administered daily at a dose of 0.625 g/kg for 2 weeks starting from 7(th) day after tumor cell transplantation. When enterosorption was completed, an analysis of peripheral blood, biochemical indices and morphological structure of tumor, lung, liver, spleen and thymus was carried out by standard methods. RESULTS: It has been shown that administration of enterosorbent did not affect LLC/R9 growth but resulted in nearly two fold decrease of the volume of lung metastases (p < 0.05). Erythrocyte number and hemoglobin level were higher by 30.0% (p < 0.05) and 23.3% (p < 0.05), respectively, in mice treated with enterosorbents as compared to untreated animals. In addition sorbent treatment completely normalized the thrombocyte index resulting in elevation of platelet number by 54.5% (p < 0.01) up to their level in intact mice. The morphological examination of liver and biochemical analysis of peripheral blood evidenced on significant positive correcting effect of enterosorption on histological structure of this organ and its functional activity. Normalization of total proteins and serum albumin level as well as significant decrease of total lipid concentration by 29% (p < 0.01) in blood of treated mice were observed. CONCLUSION: Positive influence of microgranulated carbon sorbent on some hematological, morphological and biochemical indices of tumor associated symptoms in LLC/R9-bearing mice denotes that enterosorption-based therapy can be considered as a prospective treatment for correction of some paraneoplastic syndrome signs in cancer patients.


Assuntos
Carcinoma Pulmonar de Lewis/patologia , Neoplasias Pulmonares/patologia , Neovascularização Patológica/patologia , Síndromes Paraneoplásicas/patologia , Animais , Enteroadsorção/métodos , Masculino , Camundongos , Camundongos Endogâmicos C57BL
10.
Exp Gerontol ; 19(4): 217-25, 1984.
Artigo em Inglês | MEDLINE | ID: mdl-6499964

RESUMO

The effect of repeated courses of enterosorption upon the mean and maximal lifespan and some functional and metabolic indices was determined in 28-month-old Wistar rats. Significant increase of mean and maximal lifespan of old rats was noted at certain regimens of enterosorption. The experimental animals demonstrated less marked age-related structural and ultrastructural changes in the liver, kidneys, myocardium, intestines, pancreas, as compared with control animals. Enterosorption leads to a reduction of pentobarbital-induced sleep, decrease of content of cytochrome P-450, blood cholesterol and triglycerides, cardiac and cerebral tissue cholesterol, total lipids, liver cholesterol and triglycerides. Enterosorption was found to increase the RNA and protein biosynthesis in the liver, kidneys and adrenals of old animals.


Assuntos
Carvão Vegetal/farmacologia , Absorção Intestinal , Longevidade/efeitos dos fármacos , Envelhecimento , Animais , Encéfalo/metabolismo , Carvão Vegetal/efeitos adversos , Sistema Enzimático do Citocromo P-450/metabolismo , Coração/anatomia & histologia , Hemoperfusão , Rim/anatomia & histologia , Metabolismo dos Lipídeos , Fígado/anatomia & histologia , Fígado/metabolismo , Masculino , Microscopia Eletrônica , Miocárdio/metabolismo , Ratos , Ratos Endogâmicos
11.
Eksp Onkol ; 7(3): 51-3, 1985.
Artigo em Russo | MEDLINE | ID: mdl-4017932

RESUMO

The blood flow in the rat Guerin carcinoma was measured by the hydrogen clearance method. It was shown that the blood flow in the tumour tissue decreased almost 3.5 times to the 3 hours of the intravenous glucose infusion at a dose of 80 mg X kg-1 X min-1. In some tumours the microcirculation ceased completely. The results obtained help developing the new schemes of local hyperthermia and induced hyperglycemia in the complex antitumour therapy.


Assuntos
Hiperglicemia/fisiopatologia , Neoplasias Experimentais/irrigação sanguínea , Animais , Feminino , Hidrogênio/metabolismo , Concentração de Íons de Hidrogênio , Masculino , Taxa de Depuração Metabólica/efeitos dos fármacos , Métodos , Microcirculação/fisiopatologia , Transplante de Neoplasias , Neoplasias Experimentais/metabolismo , Ratos , Ratos Endogâmicos , Fatores de Tempo
12.
13.
Int J Artif Organs ; 14(3): 179-85, 1991 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-2045194

RESUMO

The aim of this study was to clarify the relationship between the extent of activation of synthetic granula and fibrous carbons and the adsorption of bilirubin from protein solutions. The total pore volumes of granular carbons START, SCN and fibrous activated carbons ACFM were 0.9-2.2. cm3/g and 0.8-1.5 cm3/g, respectively. A parallel increase in volume and specific surface area of micropores lead to a 4-5-fold increase of bilirubin adsorption from a 3% HSA solution. About 3 mg of bilirubin per 1 ml of the working column volume is removed from the solution with a 18 mg/100 ml concentration, after 8 min contact with an adsorbent after 4 hours of perfusion. Removal of bilirubin from model solutions means the conformation of albumin molecules can be restored. Carbon adsorbents synthesized for the elimination of bilirubin from protein-containing solutions can also be called deliganding adsorbents, since under some experimental conditions they eliminate other protein-bound ligands, viz. phenols by 97-99%, bile acids by 90-92% and sodium caprylate by 89-95%.


Assuntos
Bilirrubina , Carbono , Hemoperfusão , Adsorção , Órgãos Artificiais , Humanos , Fígado , Albumina Sérica
14.
Morfologiia ; 105(11-12): 82-6, 1993.
Artigo em Russo | MEDLINE | ID: mdl-7874296

RESUMO

Changes of the connective tissue structures, appearing during all postnatal ontogenesis stages were studied in 147 human lung specimens of different age groups (from newborns up to 82-year-olds). Qualitative and quantitative composition of connective tissue structures changes with the age which leads to the lateral aggregation of the fibers and growth of the general mass of the connective tissue. Heterochronia of the age variability manifestations in different regions of the lung framework was demonstrated. The original age transformations of connective tissue structures are characteristic for the basal lung regions. With the exception of perivasal connective tissue, similar changes in the region of the lung apexes appear 3-5 years later. This gives an opportunity to distinguish three anatomic zones in the lungs in an apico-basal direction, characterising the local nature of the age changes manifestations.


Assuntos
Tecido Conjuntivo/crescimento & desenvolvimento , Pulmão/crescimento & desenvolvimento , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Envelhecimento , Criança , Pré-Escolar , Tecido Conjuntivo/enzimologia , Tecido Conjuntivo/ultraestrutura , Histocitoquímica , Humanos , Lactente , Recém-Nascido , Pulmão/enzimologia , Pulmão/ultraestrutura , Microscopia Eletrônica de Varredura , Pessoa de Meia-Idade
15.
Morfologiia ; 125(2): 69-71, 2004.
Artigo em Russo | MEDLINE | ID: mdl-15232877

RESUMO

Using osteoscopic, osteometric, radiographic and chemical methods, a study of long tubular bones (tibia) was performed to compare their parameters in Krasnoyarsk men that lived in XVII-XVIII centuries (50 observations) and at present time (30 observations). Main regularities of their variability were established taking into account the factor of time. Bones of modern men were found to have more expressed relief, greater values of osteometric parameters, among which the increase of longitudinal parameters was prevalent over the increase of width parameters and perimeters. The incidence of diaphyseal cross-sectional forms flattened in medial plane was increased, while the degree of bone mineralization was reduced with the appearance of a tendency towards decline of strength index. The data obtained demonstrate the gracilization of the skeleton of the modern city population and permit to distinguish the regional peculiarities of its variability.


Assuntos
Osso e Ossos/anatomia & histologia , Calcificação Fisiológica , Tíbia/anatomia & histologia , Adulto , Osso e Ossos/diagnóstico por imagem , Humanos , Masculino , Radiografia , Sibéria , Tíbia/diagnóstico por imagem
16.
Morfologiia ; 115(1): 31-4, 1999.
Artigo em Russo | MEDLINE | ID: mdl-10561850

RESUMO

The examination of young healthy men has allowed to establish peculiarities of metabolism and reactivity of immunocompetent cells in relation to their somatotype. In individuals with somatotypes processing high fatty component (abdominal and undetermined) total lipid content in lymphocytes is increased predominantly due to unsaturated fatty acids and cholesterol ethers; the activity of dehydrogenases (succinate dehydrogenase, malate dehydrogenase, lactate dehydrogenase, glucose-6-phosphate dehydrogenase and glutathione reductase) is increased. Cell responsiveness to emotional stress is sharply reduced. Metabolism is the most balanced, and regulatory processes are most mobile in muscular somatotype.


Assuntos
Constituição Corporal/fisiologia , Imunocompetência , Linfócitos/imunologia , Tecido Adiposo/fisiologia , Adolescente , Adulto , Ácidos Graxos Insaturados/metabolismo , Humanos , Linfócitos/ultraestrutura , Masculino , Valores de Referência , Estresse Psicológico/fisiopatologia
17.
Ukr Biokhim Zh (1978) ; 65(5): 26-30, 1993.
Artigo em Russo | MEDLINE | ID: mdl-8160293

RESUMO

Effect of lipoic acid, 5,5-dithiobis-2-nitrobenzoic acid, monoiodoacetic acid and cystine on thermal stability of human serum albumin was studied by differential scanning calorimetry and electrophoretic methods. Quantitative estimation of the protective effect of these reagents on resistance of 5% solutions of albumin to the temperature influence was studied. It is concluded that all the compounds used protect albumin from polymerization at heating.


Assuntos
Cistina/farmacologia , Ácido Ditionitrobenzoico/farmacologia , Temperatura Alta , Iodoacetatos/farmacologia , Albumina Sérica/efeitos dos fármacos , Ácido Tióctico/farmacologia , Varredura Diferencial de Calorimetria , Eletroforese , Humanos , Ácido Iodoacético , Albumina Sérica/química
18.
Vopr Med Khim ; 34(6): 23-6, 1988.
Artigo em Russo | MEDLINE | ID: mdl-3238937

RESUMO

Detection and quantitative estimation of peptides were carried out in the middle molecular fraction of blood plasma from patients with uremia using high performance liquid chromatography and thin-layer chromatography. The middle molecular fraction was separated into four fractions after ultrafiltration through a column of Protein Pak i-60. As low as 40-60 mcg/ml of the peptides were detected in the first and second fractions.


Assuntos
Toxinas Biológicas/sangue , Uremia/sangue , Proteínas Sanguíneas/análise , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Fina , Humanos , Hidrólise
19.
Med Tekh ; (4): 24-7, 1984.
Artigo em Russo | MEDLINE | ID: mdl-6482714

RESUMO

A special device is developed to purify the conserved blood from microaggregations and toxic agents. It also decreases the CO2 level and the citrate content. The device combines the properties of a microfilter and a sorption column. The specifications of the device are given.


Assuntos
Transfusão de Sangue/instrumentação , Filtros Microporos , Adsorção , Preservação de Sangue/instrumentação , Carvão Vegetal , Humanos , Tamanho da Partícula , Cloreto de Polivinila
20.
Klin Med (Mosk) ; 69(6): 51-3, 1991 Jun.
Artigo em Russo | MEDLINE | ID: mdl-1774911

RESUMO

Sixty patients with coronary heart disease, stable angina pectoris of functional class II and III and dislipoproteinemia (cholesterol greater than 6.5 mmol/l, triglycerides greater than 2.0 mmol/l, the atherogenic index greater than 3.5) were assigned to two groups. 40 patients received enterosorbent (high-porous, small-spherical fraction of CKH carbon). 20 control subjects were treated with placebo. The patients age ranged from 60 to 74. The treatment course lasted for 4 weeks. Evaluation of the treatment results showed that total cholesterol dropped by 20%, triglycerides by 27%, apoB by 32%, apoB-containing lipoproteins by 20%; there were positive changes in circulation, clinical status (in 60% of cases), exercise tolerance (by 12%). Control patients did not present any noticeable shifts. Thus, enterosorption proved an effective modality of dyslipoproteinemia correction in coronary patients of advanced age.


Assuntos
LDL-Colesterol/sangue , VLDL-Colesterol/sangue , Doença das Coronárias/sangue , Enteroadsorção , Hipercolesterolemia/terapia , Idoso , Carvão Vegetal/administração & dosagem , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Placebos
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