RESUMO
The cloned T brucei GAPDH gene was inserted within the B subtilis GAPDH gene, carried by pUC18. Upon transformation of B subtilis by this plasmid, not able to replicate in this host, the whole plasmid was inserted in the resident chromosome, presumably by a single recombination event between homologous, chromosomal and plasmid-borne sequences. The heterologous gene was expressed, as revealed by immunological reaction with monoclonal antibodies, recognizing specifically T brucei GAPDH. T brucei GAPDH, having little or no enzyme activity, comprises about 1.56% of cellular proteins. Peptide mapping showed that a fusion of a 7.5-kDa peptide had occurred to the N-terminal part of T brucei GAPDH. This fused protein is presumably the N-terminal part of B subtilis GAPDH, in agreement with the construction of the integrative plasmid.
Assuntos
Bacillus subtilis/genética , Gliceraldeído-3-Fosfato Desidrogenases/genética , Trypanosoma brucei brucei/genética , Animais , Anticorpos Monoclonais , Bacillus subtilis/enzimologia , Western Blotting , Clonagem Molecular , Expressão Gênica , Gliceraldeído-3-Fosfato Desidrogenases/metabolismo , Immunoblotting , Técnicas Imunoenzimáticas , Mapeamento de Peptídeos , Plasmídeos , Proteínas Recombinantes/metabolismo , Transformação Bacteriana , Trypanosoma brucei brucei/enzimologiaRESUMO
The effect of beta-1,3 glucan and cell walls of S. cerevisiae on one hand and of peptidoglycans from strain LDC 15 and C. renale and cell walls of C. Hofmannii on the other hand has been investigated in the experimental leprosy infection in mice. beta-1,3 glucan, by enhancing the function of the reticulo-endothelial system, is the most active inhibitor of the multiplication of Hansen's bacilli. Peptidoglycan of strain LDC 15 is less active and seems non specific.
Assuntos
Glucanos/uso terapêutico , Hanseníase/prevenção & controle , beta-Glucanas , Animais , Corynebacterium , Feminino , Camundongos , Peptidoglicano/uso terapêutico , Saccharomyces cerevisiaeRESUMO
Acid-alcohol-fast bacteria are not always detectable in all leprosy lesions. Non acid-fast microorganisms may be associated with acid-fast bacteria. The most frequently isolated strains from leprosy lesions are non acid-fast bacteria, morphologically related to C. diptheirae. Hence their designation as diphtheroïds or LDC (leprosy derived corynebacteria). Their antigenic structure is more closely related to M. leprae and other mycobacteria than to classical corynebacteria. This leads to the hypothesis of a potential role in the pathogenesis of leprosy and their use as an antigen for skin tests by leprosy patients.