RESUMO
Purified human spermatozoa do not regularly stimulate lymphocytes in spermlymphocyte cultures (SLC). This lack of consistent stimulation was found not to be dependent on sperm/lymphocyte ratio or on culture peak response time. A few weak stimulations obtained are not HLA-D or sex dependent. Contaminating seminal cells other than spermatozoa (SC non-SZ) may be responsible for the high stimulations found in SLC by others, since, in our hands, purified suspensions of SC non-SZ stimulate allogeneic lymphocytes in an HLA-D-dependent fashion, and such responses are abrogated by anti-HLA-DR monoclonal antibodies. These functional data confirm our previous finding of HLA-DR molecules on SC non-SZ by absorptions (and a lack of expression on spermatozoa) and suggest the concomitant expression of HLA-DQ and -DP products.
Assuntos
Antígenos HLA-D/metabolismo , Sêmen/imunologia , Espermatozoides/imunologia , Anticorpos Monoclonais , Feminino , Antígenos HLA-D/genética , Antígenos HLA-DR/metabolismo , Humanos , Técnicas In Vitro , Ativação Linfocitária , Teste de Cultura Mista de Linfócitos , Masculino , Sêmen/citologiaRESUMO
HLA-A and -B antigens have been found in purified spermatozoa using a double fluorescence cytotoxicity technique and specific absorptions. HLA-C, -Bw4, Bw6 and -DR antigens have not been found using the same techniques. Controversial results regarding HLA expression on spermatozoa have been presumably obtained because of a lack of both seminal cell purification and an appropriate more accurate cytotixicity technique. The presence of HLA antigens on seminal cells may be of relevance to explain the postulated immunosuppressive alloimmunization which may occur in the acquired immunodeficiency syndrome (AIDS).
Assuntos
Antígenos HLA , Espermatozoides/imunologia , Adulto , Testes Imunológicos de Citotoxicidade , Antígenos HLA-A , Antígenos HLA-B , Antígenos HLA-C , Antígenos HLA-DR , Antígenos de Histocompatibilidade Classe II , Humanos , MasculinoRESUMO
Bf allele frequencies were studied in a sample of the normal Spanish population and in family haplotypes. BfF1 shows a frequency higher than in other Caucasoid populations and closer to that found in Negroids. Basques show an even higher BfF1 frequency BfF1 is in strong linkage disequilibrium with B18. HLA-Bw44 is found to be the B12 split in linkage disequilibrium with BfF and Bw50-BfS1 association is confirmed. DR3--BfF1 are not in linkage disequilibrium in the normal Spanish population, in contrast to DR3--BfF1 in linkage found in a diabetic Spanish population. Results are discussed on the bases of the paleo-North African Iberian population origins and of the use of Bf to define B12 and Bw21 splits.
Assuntos
Fator B do Complemento/genética , Precursores Enzimáticos/genética , Frequência do Gene , Antígenos HLA/genética , Polimorfismo Genético , Alelos , Ligação Genética , Haploidia , Teste de Histocompatibilidade , Humanos , Fenótipo , EspanhaRESUMO
HLA-A, HLA-B, HLA-C, HLA-Bw4, Bw6 and HLA-DR antigens have been detected on purified seminal cells than spermatozoa (SCnonSz) using specific absorptions. These results contrast with those obtained on spermatozoa, which only express significant amounts of HLA-A and HLA-B antigens, and which may be relevant to explain immunosuppressive alloimmunization in the acquired immunodeficiency syndrome (AIDS).