RESUMO
BACKGROUND: Toxoplasma gondii populations that perpetuate in South America's natural ecosystems display broad genetic diversity, but the impact of this diversity on humans is generally unknown. In this short communication, we depict the genotypic traits of four isolates related to congenital parasitism as it emerges in Southern Brazil. METHODS: Using the PCR-restriction fragment length polymorphism markers SAG1, 5'3'SAG2, alt. SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico, and CS3. Three of the four strains (TgCTBral, TgCTBrv, and TgCTBrac) were identified as ToxoDB genotype #166. RESULTS: Three children displayed normal cognitive/psychomotor development, and one child displayed Sabin's tetrad (TgCTBral). CONCLUSION: Vertical transmission of the two genotypes was observed and contributes to knowledge of T. gondii strains isolated from humans in Brazil.
Assuntos
Toxoplasma/genética , Toxoplasmose/parasitologia , Adulto , Brasil , Criança , Transtornos Cognitivos/etiologia , DNA de Protozoário/genética , Deficiências do Desenvolvimento/etiologia , Feminino , Seguimentos , Marcadores Genéticos , Variação Genética , Genótipo , Humanos , Transmissão Vertical de Doenças Infecciosas , Reação em Cadeia da Polimerase/métodos , Polimorfismo de Fragmento de Restrição , Gravidez , Complicações Parasitárias na Gravidez/parasitologia , Toxoplasma/isolamento & purificação , Toxoplasmose/complicações , Toxoplasmose/transmissãoRESUMO
PURPOSE: To analyze the late results of patients who underwent left coronary myocardial revascularization with both internal thoracic arteries, with the right internal thoracic through the transverse sinus. METHODS: From July/83 to September/96, 233 patients underwent myocardial revascularization, with ages ranging from 35 to 76 (average of 52.8) years. One hundred and eighty five were male and 48 female. Internal thoracic arteries (466), saphenous veins (192) and epigastric arteries (11) were employed. RESULTS: The hospital mortality was 3and the late mortality was 6.1. The immediate postoperative bleeding was 0.8, mediastinitis 1.7and myocardial infarction 2.1. The immediate and late coronariography showed the rates of patency, respectively: left internal thoracic artery 98and 96, right internal thoracic artery 96and 92, and saphenous vein grafts 80and 67.5. CONCLUSION: The main advantage in using both internal thoracic arteries in the revascularization of the left coronary branches was the better quality of life, the low rates of cardiac events and the absence of reoperation due to occlusion of the grafts.