Investigating the Physiological and Molecular Responses of Solanum lycopersicum hp Mutants to Light of Different Quality for Biotechnological Applications.

The effect of the light of different spectral compositions, white fluorescent light (WFL), red light (RL, 660 nm), blue light (BL, 450 nm), green light (GL, 525 nm), and white LED light (WL, 450 + 580 nm), on the physiological parameters of Solanum lycopersicum 3005 hp-2 (defective for a DET1 gene) and 4012 hp-1w; 3538 hp-1; 0279 hp-1.2 (defective for a DDB1a gene) photomorphogenetic mutants was studied. The parameters of the primary photochemical processes of photosynthesis, photosynthetic and transpiration rates, the antioxidant capacity of low-molecular weight antioxidants, the content of the total phenolic compounds, including flavonoids, and the expression of the genes involved in light signaling and biosynthesis of secondary metabolites were determined. Under BL, the 3005 hp-2 mutant showed the highest nonenzymatic antioxidant activity, which occurred to a greater extent due to the increase in flavonoid content. At the same time, under BL, the number of secretory trichomes on the surface of the leaves of all mutants increased equally. This suggests the accumulation of flavonoids inside leaf cells rather than in trichomes on the leaf surface. The data obtained indicate the possibility of using the hp-2 mutant for biotechnology to increase its nutritional value by enhancing the content of flavonoids and other antioxidants by modulating the spectral composition of light.

Megasporogenesis, megagametogenesis and embryogenesis of Liparis elliptica (Orchidaceae), with special note to the development of unique unitegmal ovule.

Megasporogenesis, megagametogenesis and embryogenesis of Liparis elliptica (family Orchidaceae, tribe Malaxideae, subtribe Malaxidinae) have been studied. It was shown that the L. elliptica embryo sac is monosporic and develops from the chalazal cell of the megaspore triad according to the modified Polygonum type. The embryo sacs are reduced to four-six nuclei. The suspensor is unicellular, spherical in shape, originating from the basal cell (cb). A unique feature of L. elliptica is the unitegmal ovule, which distinguishes this species from other members of the tribe Malaxideae. The seed coat is formed by an outer layer of the single internal integument. Reduction of the outer integument is a rare feature for epiphytic orchid species with photosynthetic leaves.

Suspensor differentiation in the embryogenesis of Liparis from sections Cestichis and Blepharoglossum (Malaxidinae, Orchidaceae).

Embryological features were used for the first time as a taxonomic attribute to confirm the difference between closely related taxa in the subtribe Malaxidinae. It was shown that the branched shape of the suspensor in Liparis elegans and L. parviflora from the section Blepharoglossum distinguishes these species from the inflated suspensor without a neck, which is characteristic of the embryos of L. viridiflora and L. dendrochiloides from the section Cestichis. Differentiation of the development and shape of the suspensor is an additional embryological criterion in favor of separating the genus Blepharoglossum from the genus Liparis.

Micromorphology of the leaf surface in some species of Dryadoideae (Rosaceae).

The leaf surface of 5 species of the subfamily Dryadoideae (Rosaceae) was studied for the first time by cryoscanning electron microscopy. In the studied representatives of Dryadoideae, some signs of micromorphology were found that are characteristic of other Rosaceae. In Dryas drummondii and D. x suendermannii, cuticular folding was found on the cell surface of the adaxial leaf side. Stomatal dimorphism was found in Cercocarpus betuloides. A representative of the genus Cercocarpus had pronounced differences from the species of the genus Dryas in less pubescence of the abaxial surface with shorter and thicker trichomes, in small elongated stomata, and in smaller cells of the adaxial epidermis. Glandular trichomes and long multicellular outgrowths (possibly emergences) were found on veins in D. grandis. Structures resembling hydathodes or nectaries have also been noted on the leaf margin in this species.

Megasporogenesis, megagametogenesis, and embryogenesis in Maxillaria crassifolia (Lindl.) Rchb.f. (Cymbidieae, Orchidaceae).

Maxillaria crassifolia (Lindl.) Rchb.f. belongs to the polyphyletic genus Maxillaria Ruiz & Pav., which currently is the subject of several taxonomic research. There are conflicting descriptions of megasporogenesis, megagametogenesis, and embryogenesis in orchids from the tribe Cymbidieae, in general, and in the genus Maxillaria, in particular. In the present report, all stages of embryonic development of M. crassifolia were examined using confocal fluorescence microscopy. Some features of the development of the ovule and embryo, which distinguish M. crassifolia from other species of the tribe Cymbidieae were identified. The T-shaped arrangement of megaspores is formed by dividing the micropylar megaspore of the dyad. The megagametophyte develops according to the modified Polygonum-type with an unstable number of nuclei in the embryo sacs. The nucleus of the central cell varies in composition and may include unfused micropylar and chalazal nuclei and daughter nuclei formed during their division. The sequence of embryonal divisions is strictly structured. A special variant of embryogenesis, the Cymbidium-type Maxillaria-variant, has been described. Its characteristic features are the strictly apical nature of embryonic divisions, the absence of basal cell (cb) division, the formation of one to three pairs of tubular suspensor cells, and the localization of all suspensor cells within the inner integument.

Outer and internal cuticle in the leaves of Malus (Rosaceae) in mountains and plains.

The outer and internal cuticles in apple (Malus domestica Borkh.) leaves on the plain and in the mountains was studied using transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM). The outer cuticle consisted of lamellate and homogeneous layers of the cuticle proper and cuticular layer containing electron-transparent plates and electron-dense dendrites. Blue fluorescence predominated in the cell wall. The cuticle fluoresced green and red. The intensity of the red part of the spectrum in the cuticle increased with altitude, and the number of electron-transparent plates increased within the cuticular layer. The cell wall on both leaf sides was the thinnest in the arid conditions (300 m). At an altitude of 600 m, under favorable temperature and rainfall conditions, the cuticle thickness increased due to the cuticular layer adjacent to the cell wall. The internal cuticle was distinguished by intense yellow or red autofluorescence, similar in color and spectrum to the outer cuticle. The outer and internal cuticles had cuticular folds. The average distance between the ridges of the internal cuticle was almost the same in the samples at different altitudes. The ridge height was maximum at 600 m. RESEARCH HIGHLIGHTS: The altitudinal effects on outer and internal cuticles in apple leaves were studied. In the forest-mountain zone, the cuticle thickness increased due to the cuticular layer adjacent to the cell wall. The cell wall was the thinnest in arid conditions.

Effect of Phytochrome Deficiency on Photosynthesis, Light-Related Genes Expression and Flavonoid Accumulation in Solanum lycopersicum under Red and Blue Light.

The effect of red (RL, 660 nm) and blue (BL, 450 nm) light on phy mutant tomato plants was studied. The rates of photosynthesis (Pn) and transpiration, the efficiency of the primary photochemical processes of photosynthesis, the contents of flavonoids and phenolic compounds, the low-molecular-weight antioxidant capacity (Trolox equivalent antioxidant capacity (TEAC)) of leaf extracts, and the expression of light-dependent genes were evaluated. Under RL, BL, and white fluorescent light (WFL), the Pn values decreased in the order: WT > phyb2 > phyaphyb2 > phyaphyb1phyb2, except for the Pn in phyb2 on BL. Phyb2 also had a larger number of stomata under BL and, as a result, it reached maximum transpiration. The noticeable accumulation of flavonoids and phenolic compounds was observed only in the phyb2 and phyaphyb2 mutants upon irradiation with BL, which agrees with the increased TEAC in the leaf extracts. We suggest that the increased antioxidant activity under PHYB2 deficiency and the maintenance of high photosynthesis under BL are based on an increase in the expression of the early signaling transcription factors genes BBX, HY5. The largest decrease in the content of flavonoids and TEAC was manifested with a deficiency in PHYB1, which is probably the key to maintaining the antioxidant status in BL plants.

Megasporogenesis, megagametogenesis, and embryogenesis in Dendrobium nobile (Orchidaceae).

The orchid reproductive strategy, including the formation of numerous tiny seeds, is achieved by the elimination of some stages in the early plant embryogenesis. In this study, we documented in detail the formation of the maternal tissues (the nucellus and integuments), the structures of female gametophyte (megaspores, chalazal nuclei, synergids, polar nuclei), and embryonic structures in Dendrobium nobile. The ovary is unilocular, and the ovule primordia are formed in the placenta before the pollination. The ovule is medionucellate: the two-cell postament and two rows of nucellar cells persist until the death of the inner integument. A monosporic eight-nucleated embryo sac is developed. After the fertilization, the most common central cell nucleus consisted of two joined but not fused polar nuclei. The embryogenesis of D. nobile is similar to the Caryophyllad-type, and it is characterized by the formation of all embryo cells from the apical cell (ca) of a two-celled proembryo. The only exception is that there is no formation of the radicle and/or cotyledons. The basal cell (cb) does not divide during the embryogenesis, gradually transforming into the uninuclear suspensor. Then the suspensor goes through three main stages: it starts with an unbranched cell within the embryo sac, followed by a branched stage growing into the integuments, and it ends with the cell death. The stage-specific development of the female gametophyte and embryo of D. nobile is discussed.