Visual conditions affecting eye growth alter diurnal levels of vitreous DOPAC.

In chicks, the diurnal patterns of retinal dopamine synthesis and release are associated with refractive development. To assess the within-day patterns of dopamine release, we assayed vitreal levels of DOPAC (3,4-dihydroxyphenylacetic acid) using high performance liquid chromatography with electrochemical detection, at 4-h intervals over 24 h in eyes with experimental manipulations that change ocular growth rates. Chicks were reared under a 12 h light/12 h dark cycle; experiments began at 12 days of age. Output was assessed by modelling using the robust variance structure of Generalized Estimating Equations. Continuous spectacle lensdefocus or form deprivation: One group experienced non-restricted visual input to both eyes and served as untreated "normal" controls. Three experimental cohorts underwent monocular visual alterations known to alter eye growth and refraction: wearing a diffuser, a negative lens or a positive lens. After one full day of device-wear, chicks were euthanized at 4-h intervals over 24 h (8 birds per time/condition). Brief hyperopic defocus: Chicks wore negative lenses for only 2 daily hours either in the morning (starting at ZT 0; n = 16) or mid-day (starting at ZT 4; n = 8) for 3 days. Vitreal DOPAC was assayed. In chicks with bilateral non-restricted vision, or with continuous defocus or form-deprivation, there was a diurnal variation in vitreal DOPAC levels for all eyes (p < 0.001 for each). In normal controls, DOPAC was highest during the daytime, lowest at night, and equivalent for both eyes. In experimental groups, regardless of whether experiencing a growth stimulatory input (diffuser; negative lens) or growth inhibitory input (positive lens), DOPAC levels were reduced compared both to fellow eyes and to those of normal controls (p < 0.001 for each). These diurnal variations in vitreous DOPAC levels under different visual conditions indicate a complexity for dopaminergic mechanisms in refractive development that requires further study.

Analysis of Kinematic Response of Pediatric Occupants Seated in Naturalistic Positions in Simulated Frontal Small Offset Impacts: With and Without Automatic Emergency Braking.

Naturalistic driving studies have shown that pediatric occupants do not assume ideal seating positions in real-world scenarios. Current vehicle assessment programs and child restraint system (CRS) sled tests, such as FMVSS No. 213, do not account for a wide range of seating postures that are typically observed during real-world trips. Therefore, this study aims to analyze the kinematic and kinetic response of a pediatric human body model in various naturalistic seating positions in booster seats when subjected to a frontal offset impact in a full-vehicle environment, with and without the application of pre-crash automatic emergency braking (AEB). A 6YO (seated on a lowback and highback booster) and a 10YO (seated in no-CRS and on a lowback booster) PIPER pediatric human body model's response was explored in a reference, and two most commonly observed seating postures: forward-leaning and forward-inboard-leaning. The vehicle environment with a side-curtain airbag (SCAB) was subjected to a small offset barrier impact (25% overlap at 40MPH), with and without the application of a pre-crash automatic emergency braking (AEB). 24 conditions were simulated using finite element analysis. Cases with a pre-crash AEB resulted in relatively lower kinematic and kinetic values due to the occupant being in a more flexed position before impact compared to without-AEB cases, coupled with the increased ride-down effect due to AEB. Moreover, different seating postures resulted in substantially different kinematics and kinetics, the injury metrics crossing the injury assessment reference values in some cases. Therefore, to design a passive safety standard test for pediatric occupants, it is important to consider the possible postural changes that may occur.