Indicators of chemiluminescent and bioluminescent tests of biological liquids in the assessment of physical health.

The study includes anthropometry of 172 young male, obtained data on the length and body mass, measured the transverse diameters of the shoulders and pelvis, various body types was identified by the J.M. Tanner sexual dimorphism index (andromorphic, mesomorphic, gynecomorphic). The chemiluminescent and bioluminescent study of saliva and blood was conducted in the examined young male. We studied the indicators of the antioxidant defense system under the influence of stress. The antioxidant status of saliva was determined using the H2O2-luminol-dependent chemiluminescence method. Data on the activity of NAD (P) -dependent dehydrogenases in blood lymphocytes was obtained from a bioluminescent method of research. Young male of andromorphic body type had large overall and transverse body sizes. Indicators of antioxidant protection of saliva and blood in men of adolescence, the body type of the sexual dimorphism index J.M. Tanner was different. The persons of the andromorphic body type differed in terms of chemiluminescence in comparison with the young male of gynecomorphic body type. The results of bioluminescent blood tests suggest a violation of the catabolic and anabolic processes of carbohydrate and fat metabolism in young men of mesomorphic and gynecomorphic body types. Indicators of the system of antioxidant protection of saliva and blood reflect the sexual characteristics of the body of young male and can be used as additional criteria for diagnosing sex inversion and assessing the risk of developing socially attributed diseases.

[Transport processes of fluid exchange in the crystalline lens of the rabbit eye].

Processes of fluid exchange in the crystalline lens of the rabbit eye were investigated. The direction of movement of fluid in the crystalline lens was investigated from the movement of fluorescein by the method of "stopped diffusion". It has been found that the mechanism of fluid transport in the crystalline lens is active and is carried out by means of the Na-kappa-ATPase transport system. The energy necessary for the active transport of fluid inside the crystalline lens is in the range (1.5-6) x 10(-2) J. Owing to the active fluid transport, the pressure inside the crystalline lens constantly increases by 6 mm Hg. In rabbit's life-time, the movement of fluid in the crystalline lens occurs in the direction from the anterior to the posterior surface followed by the exit to vitreous humor.

[Effects of aggressive therapy with simvastatin in patients with acute coronary syndrome and initially normal level of LDLP cholesterol on cardiovascular outcomes (LAOKOON). Pilot randomized trial].

AIM: To examine anti-inflammatory effect of statins on outcomes of acute coronary syndrome (ACS). MATERIAL AND METHODS: The trial included 211 ACS patients without ST elevation at ECG. The patients were followed up for 180 days. The anti-inflammatory action was studied for 30 days. The patients were divided into two groups by the treatment: simvastatin 10 mg + aspirin 325 mg (104 patients, group 1) and simvastatin 40 mg + aspirin 325 mg (107 patients, group 2) for 30 days. RESULTS: Simvastatin in maximal dose lowed LDLP cholesterol from 101 +/- 9.8 to 72 +/- 3.3 mg/dl, in a minimal dose--from 104 +/- 11.3 to 81 +/- 2.1 mg/dl (p < 0.05). Only maximal dose simvastatin produced a significant reduction of C-reactive protein (CRP) to the disease day 14. The integral index including all cases of cardiovascular death +acute myocardial infarction+progressive angina+rehospitalization was 70 scores for group 1 and 137 for group 2 (p = 0.047). CONCLUSION: Use of simvastatin in ACS patients, initially normal level of LDLP cholesterol and elevated level of CRP produced a dose-dependent effect, alleviated inflammation and improved the disease course.

Harderian gland ultrastructure of the black sea bottlenose dolphin (Tursiops truncatus ponticus).

Examination of the Harderian gland structure of the Black Sea bottlenose dolphin, Tursiops truncatus ponticus, at macroscopic, microscopic, and electron microscopic levels shows significant sexual dimorphism. The epithelial cells of male and female glands are different cell types, capable of producing chemically different products. Secretory cells in both sexes contain secretion granules that produce a secretion consisting mainly of proteins and carbohydrates, but thought to be sex-specific in composition. The female glands also contain lipid secretion granules. It is suggested that in the bottlenose dolphin the Harderian gland functions to produce sexually distinct pheromones and may have other physiological activities, e.g., participating in local immunological or endocrine-related reactions.

[Effect of vitamin A on the adhesive interaction of cells in epithelial tissue]. / Vliianie vitamina A na adgezivnye vzaimodeistviia kletok v épitelial'nykh tkaniakh.

Both the increasing and decreasing contents of vitamin A in rats induced a lowering of cell adhesion in the skin multilayer epithelium, and that of their packing density. However, the same stimulus did not alter cell adhesion in the small intestinal monolayer epithelium. The data obtained show that vitamin A may serve as a factor modulating intercellular adhesive interactions with tissue specificity.

[Content and distribution of water and potassium in the body of patients with chronic circulatory insufficiency]. / Soderzhanie i raspredelenie vody i kaliia v organizme bol'nykh s khronicheskoi nedostatochnost'iu krovoobrashcheniia

The total water content (after antipyrine and tritium oxide), that of extracellular water (after sodium thiosulphate) and of overall metabolic potassium (with reference to K42) were studied concurrently in 34 patients with chronic circulatory insufficiency. Twenty normal subjects served as controls. In the sick the disturbances of the water metabolism made themselves felt not only in the accumulation of surplus water in the body, but also in its re-distribution. In the presence of marked edemas the total water level rises both at the expense of extra- and intracellular water. With the normal level of total water the extracellular water fraction is up and the intracellular one is down. An early and consistent sign of circulatory insufficiency is a drop in the amount of overall metabolic potassium in the body. The potassium deficit increases parallel with progressing circulatory insufficiency, being contingent not only upon the reduction of the cellular mass, but also on the lower intracellular concentration of potassium. The concentration of potassium in the plasma does not reflect the overall content of it in the body.

[The lipid-secreting skin glands in the common hamster (Cricetus cricetus Linnaeus, 1758)]. / Lipidsekretiruiushchie kozhnye zhelezy obyknovennogo khomiaka (Cricetus cricetus Linnaeus, 1758).

The Harderian gland and glands of the oral angle structure and processes of secretion in the common hamster have been studied under light and electron (scanning and transmission) microscopes. Both these glands were shown to be lipid-producing. Nevertheless, their epithelial cells differ in ultrastructure. The Harderian gland has tubulo-alveolar architecture and consists of two types of epithelial cells. The oral angle gland is acinar and contains differentiating cells of the third type. The possibility of synthesis of various lipids by these three types of cells is discussed.

[The morphofunctional characteristics of the harderian gland in some mammalian species]. / Morfofunktsional'nye osobennosti garderovoi zhelezy nekotorykh vidov mlekopitaiushchikh.

The structure of harderian glands was studied using electron microscopy and histochemistry in six rodent species, including three Phodopus species. Based on the concept of cell morphotype it was shown that the harderian glands of the studied mammalian species are lipid-secreting and have secretory cells of the species specific morphotypes. The secretory cells are distinguished by the ultrastructural features not only between different genera, but also between different species of the same genus. The data obtained allow species identification according to the ultrastructure of secretory cells. The role of harderian glands in chemical communication was shown for the golden hamster (Mesocricetus auratus). Their secretory substance contains information about the sexual and species identity of animals.

[Brain glycolipids in experimental brain edema]. / Glikolipidy mozga pri ego éksperimental'nom oteke.

Quantitative and qualitative composition of gangliosides, sulfatides and cerebrosides were studied in normal state and in experimental brain edema by means of thin-layer chromatography. In the experimental edema content of mono- and disialogangliosides was decreased while tri- and tetrasialogangliosides were unaltered. Statistically distinct increase in content of sulfocerebrosides (sulfatide 1.2) was also detected in brain edema as compared with controls. At the same time, amount of cerebroside 2 was decreased, whereas increase in cerebroside I concentration was detected.

[Methods of hormone replacement therapy in hypertensive women]. / Taktika zamestitel'noi gormonal'noi terapii u zhenshchin s arterial'noi gipertenziei.

A comparative retrospective study was made to examine influence of replacement therapy by estradiol valeriate and climonorm on perimenopausal hypertension. The test group (25 hypertensive women) received climonorm for 6 months. Control women received hypotensive therapy. Lipid metabolism and risk of vascular events were assessed. The replacement therapy had a positive effect on lipid metabolism. Criteria of Mann-Whitney, Friedmann, Wilkinson and of chi 2 were made use of. The target arterial pressure was achieved only with hypotensive therapy which also diminished intensity of menopausal disorders. To determine individual policy, it is necessary to stratify risk of cardiovascular events. The best effect is achieved at combination of hypotensive therapy with replacement hormone treatment.