RESUMO
This study determined the in vivo interproximal bactericidal efficacy of an essential oil-containing antiseptic mouthrinse (Listerine Antiseptic) following toothbrushing. Thirty-four generally healthy adults, aged 23-64 years, completed this evaluator-blind, randomized, controlled, crossover designed study. Subjects rinsed for 30 seconds with 20 ml of either the essential oil rinse or a negative control rinse. Five minutes later, interproximal plaque was collected using paper points, and recoverable bacterial counts were quantified using both end point dilution and spectrophotometric methods. The results from the end point dilution demonstrated a statistically significant (p < 0.001) 43.8% reduction in recoverable plaque bacteria from interproximal spaces following rinsing with the essential oil mouthrinse. This study suggests that the clinical effectiveness of the essential oil mouthrinse against plaque and gingivitis may be attributable to the rapid kill and plaque permeabilizing properties of the formulation.
Assuntos
Anti-Infecciosos Locais/farmacologia , Placa Dentária/microbiologia , Antissépticos Bucais/farmacologia , Salicilatos/farmacologia , Streptococcus mutans/efeitos dos fármacos , Terpenos/farmacologia , Adulto , Biofilmes/efeitos dos fármacos , Contagem de Colônia Microbiana , Estudos Cross-Over , Combinação de Medicamentos , Humanos , Análise dos Mínimos Quadrados , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Antissépticos Bucais/química , Análise Multivariada , Óleos Voláteis/farmacologia , Salicilatos/química , Método Simples-Cego , Terpenos/químicaRESUMO
Although laboratory studies are not necessarily predictive of clinical activity; they can help to elucidate mechanisms underlying clinical activity when the latter has been established. In a recent clinical study, an essential oil mouthrinse (Listerine Antiseptic) was shown to be significantly more effective than an amine fluoride/stannous fluoride mouthrinse (Meridol) in inhibiting supragingival plaque formation. This paper reports the results of laboratory studies comparing the antimicrobial effectiveness of these 2 mouthrinses using a kill kinetics assay and a plaque biofilm kill assay. In both assays, the essential oil mouthrinse was considerably more effective than the amine fluoride/stannous fluoride mouthrinse. These findings are consistent with the results of the clinical trial and may help to explain the observed differences in clinical activity.
Assuntos
Aminas/farmacologia , Anti-Infecciosos Locais/farmacologia , Bactérias/efeitos dos fármacos , Antissépticos Bucais/farmacologia , Óleos Voláteis/farmacologia , Salicilatos/farmacologia , Terpenos/farmacologia , Fluoretos de Estanho/farmacologia , Biofilmes/efeitos dos fármacos , Candida albicans/efeitos dos fármacos , Corantes , Placa Dentária/microbiologia , Combinação de Medicamentos , Fusobacterium nucleatum/efeitos dos fármacos , Humanos , Lacticaseibacillus casei/efeitos dos fármacos , Prevotella intermedia/efeitos dos fármacos , Saliva/microbiologia , Streptococcus mutans/efeitos dos fármacos , Língua/microbiologiaRESUMO
Using capillary electrophoresis, the enantiomers of a number of dansyl amino acids were resolved using nativeß-cyclodextrin. The neutral chiral host resolved analytes possessing a negative charge at pH 9, the conditions employed in this study. Organic modifiers added to the running buffer were particularly adept at enhancing chiral recognition between the guest and host molecule in capillary electrophoresis. This work examined the effects of methanol, dimethylformamide, and acetonitrile on the resolution, migration time, and efficiency of twelve dansyl amino acids. Examples are given of the separation of racemic dansyl amino acids utilizing this technique and conditions necessary to achieve enantioselectivity.