[Ultrastructure of parasitic forms of the agent of coccidioidomycosis]. / K ul'trastrukture paraziticheskikh form vozbuditelia koktsidioidnogo mikoza

The authors studied the ultrastructure of the Coccidiodes immitis spherules in the sections of the abdominal cavity foci and pneumonic foci of Cricetus auratus W. and in albino mice infected intraperitoneally with a lethal dose of the mycelium fungus phase. It was found that the spherules of coccidiod fungus represented multinuclear cells with a well-organized protoplast included into a complicated supermembrane structure--the cell wall. Endospores (which copied the spherule structure in miniature) formed as a result of division of the cytoplasm by entire sections into polygonal segments with the subsequent separation of the latter. Due to the rupture of the cell wall of mature sporulating spherules the endospores are released into the surrounding tissue and are attacked there and phagocytized by polynuclears and macrophages. Phygocytosis of the fungi is mostly incomplete.

[Morphogenesis of experimental primary pulmonary coccidioidal mycosis]. / Morfogenez éksperimental'nogo pervichnogo legochnogo koktsidioidnogo mikoza

Morphogenesis of experimental primary pulmonary coccidioidomycosis in white mice was studied. The aspirated particles of the mycelium by the 5th day formed sporilating sporangiums, the latter being the main cause of a specific inflammatory process. Three stages in the development of the infectious process were singled out: incubation, in the course of which accumulation of the pathogene in the lungs took place (the first seven days), initiation and progressing of pneumonia, culminated with the animals' perish in the course of 12-14 days.

[Electron microscopic study of the small intestine in experimental cholera]. / Elektronno-mikroskopicheskoe issledovanie tonkogo kishechnika pri éksperimental'noi kholere.

Ultrastructure of the small intestine was studied in nursling rabbits infected with the El Tor 5879 vibrio culture. Cholera vibrio was localized in the intestinal lumen mostly in the crypts area, in some places adhering to the brush edge of enterocytes without penetrating them. Epithelium of the villae remained intact; it however displayed dystrophic changes of microvilli and organoids, cytoplasma vacuolization and increase in lysosome count. The mentioned changes pointed to a marked elevation of functional enterocyte activity in cholera.

[Ultrastructural changes in plague microbes of vaccinal strain EB and alveolar macrophages of guinea pigs during their interaction in vitro]. / Ul' trastrukturnye izmeneniia v chumnykh mikrobakh vaktsinnogo shtamma EB i al'veoliarnykh makrofagakh morskikh svinok pri vzaimodeistvii ikh in vitro.

Aerosol immunization with plague vaccine EB was found to stimulate an increase in the number of free alveolar macrophages and to enhance their ingesting activity which correlated with an increase in specific antibody titers. Immunological transformation was accompanied by ultrastructural changes in the cytoplasm and the nuclei of alveolar macrophages. The phagocytosis of microbes occurred by their adsorption onto the microphage surface, invagination and enclosing into the endocytic bubble having the function of the digestive vacuole. The growth of the number of macrophages and an increase in their ingesting activity, progressive changes in the cytoplasmic structures may be used as indicators of immunological transformation resulting from the administration of plague vaccine by the inhalation method.