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1.
Mol Biol (Mosk) ; 51(1): 126-130, 2017.
Artigo em Russo | MEDLINE | ID: mdl-28251975

RESUMO

Shallot virus X is a typical representative of Allexiviruses. The transcription levels of principal genes involved in the RNA silencing in healthy and shallot virus X-infected plants have been quantified by real-time polymerase chain reaction. There is a negative correlation between the reproduction rates of RNA virus and the levels of RNA-dependent RNA polymerase and DCL proteins in roots and leaves of infected plants. These observations indicate that Shallot X virus employs noncanonical ways of overcoming the antiviral defense of the plant by systemic RNA silencing.


Assuntos
Flexiviridae/patogenicidade , Doenças das Plantas/virologia , Raízes de Plantas/virologia , RNA Polimerase Dependente de RNA/genética , Células Vegetais/virologia , Doenças das Plantas/genética , Raízes de Plantas/genética , Interferência de RNA , RNA de Plantas
2.
Biochemistry (Mosc) ; 77(9): 1072-81, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23157268

RESUMO

The N-terminal half of TGB1 movement protein of poa semilatent hordeivirus, which forms a ribonucleoprotein complex involved in movement of the viral genome in the plant, and its two domains, NTD and ID, are phosphorylated in vitro by a fraction enriched in cell walls from Nicotiana benthamiana. Using a set of protein kinase inhibitors with different specificities, it was found that enzymes possessing activities of casein kinase 1, protein kinase A, and protein kinase C are involved in phosphorylation. Commercial preparations of protein kinases A and C are able to phosphorylate in vitro recombinant proteins corresponding to the N-terminal half of the protein and its domains NTD and ID. Phosphorylation of the NTD has no effect on the efficiency and character of its binding to RNA. However, phosphorylation of the ID leads to a decrease in its RNA-binding activity and in the ability for homological protein-protein interactions.


Assuntos
Proteínas do Movimento Viral em Plantas/química , Proteínas do Movimento Viral em Plantas/metabolismo , Caseína Quinase I/antagonistas & inibidores , Caseína Quinase I/metabolismo , Parede Celular/química , Proteínas Quinases Dependentes de AMP Cíclico/antagonistas & inibidores , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Fosforilação/efeitos dos fármacos , Proteínas do Movimento Viral em Plantas/antagonistas & inibidores , Proteínas do Movimento Viral em Plantas/genética , Vírus de Plantas , Proteína Quinase C/antagonistas & inibidores , Proteína Quinase C/metabolismo , Inibidores de Proteínas Quinases/farmacologia , Nicotiana/citologia , Nicotiana/virologia
9.
Biochemistry (Mosc) ; 73(1): 50-5, 2008 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-18294129

RESUMO

A 25-kD movement protein (25K protein) encoded by the first gene of the potexvirus Potato virus X triple gene block of transport genes is essential for the viral movement in infected plants. The 25K protein belongs to superfamily 1 of NTPase/helicases and exhibits in vitro RNA helicase, Mg2+-dependent NTPase, and RNA-binding activities. In the present work, the ability of 25K protein for homologous interactions was studied using the yeast two-hybrid system, protein chemical cross-linking in the presence of glutaraldehyde, far-Western blotting, and ultracentrifugation in sucrose density gradients. The 25K protein was shown to form homodimers and homooligomers. Sites of homologous protein-protein interactions were found in both the N- and C-terminal portions of the protein.


Assuntos
Proteínas do Movimento Viral em Plantas/química , Potexvirus , Dimerização , Glutaral/química , Proteínas do Movimento Viral em Plantas/metabolismo , Técnicas do Sistema de Duplo-Híbrido , Ultracentrifugação
10.
Arch Virol ; 146(6): 1213-7, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11504426

RESUMO

Flexible filamentous shallot virus X (ShVX) particles were detected in extracts of Beta vulgaris protoplasts inoculated with transcripts from a full-length ShVX cDNA. Extracts from ShVX-infected protoplast were infectious for ShVX-healthy shallot seedlings. Western blot analysis of inoculated plants revealed the accumulation of the ShVX coat protein, while electron microscopy confirmed the presence of ShVX virions. The results suggest that the in vitro RNA transcripts from full-length ShVX cDNA give rise to infectious viral particles.


Assuntos
Chenopodiaceae/virologia , Vírus de Plantas/genética , Vírus de Plantas/fisiologia , Vírus de RNA/genética , Vírus de RNA/fisiologia , Allium/virologia , Capsídeo/metabolismo , DNA Complementar/genética , DNA Viral/genética , Microscopia Imunoeletrônica , Vírus de Plantas/ultraestrutura , Protoplastos/virologia , Vírus de RNA/ultraestrutura , Montagem de Vírus
11.
J Gen Virol ; 73 ( Pt 10): 2553-60, 1992 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-1339468

RESUMO

The 8890 nucleotide RNA sequence of shallot virus X (ShVX), a new virus isolated from shallot, has been determined. The sequence contains six open reading frames (ORFs) which encode putative proteins (in the 5' to 3' direction) of M(r) 194528 (ORF1), 26333 (ORF2), 11245 (ORF3), 42209 (ORF4), 28486 (ORF5) and 14741 (ORF6). The ORF1 protein was found to be highly homologous to the putative potexvirus RNA replicases; ORF2, -3, -5 and -6 proteins also have analogues among the potex- and/or carlavirus-encoded proteins. ORF3 is followed by an AUG-lacking frame coding for an amino acid sequence homologous to that of the 7K to 8K proteins of the triple gene block of the above-mentioned viruses. The putative ORF4 protein has no reliable homology with proteins in the database. The results obtained testify that, except for the unique 42K protein gene, the ShVX genome combines a number of elements typical of both carla- and potexviruses.


Assuntos
Genes Virais/genética , Genoma Viral , Vírus de Plantas/genética , Vírus de RNA/genética , Proteínas Virais/genética , Proteínas Estruturais Virais/genética , Sequência de Aminoácidos , Sequência de Bases , Capsídeo/genética , Clonagem Molecular , DNA Helicases/genética , Dados de Sequência Molecular , Fases de Leitura Aberta , Vírus de Plantas/classificação , Plantas/microbiologia , RNA Polimerase Dependente de RNA/genética , Homologia de Sequência de Aminoácidos
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