An examination of two different approaches for the study of femoral neck fracture: Towards a more relevant rodent model.

Femoral neck fractures are a massive personal and health programme burden. Methods to study femoral neck strength, across its combined trabecular and cortical components are therefore essential. Rodent ovariectomy-induced osteoporosis models are commonly coupled with ex vivo 3-/4-point bending methods to measure changes in femoral cortical diaphysis. The loading direction used to assess these properties are often non-physiologic and, moreover, these ovariectomy models are linked to marked weight gain that can influence the biomechanical properties. Herein, we explore whether more physiological axial ex vivo loading protocols applied to femoral neck samples of ovariectomised (OVX) rodents provide anatomically-relevant models for the assessment of strength. We examine the use of mouse and rat femurs, loaded in constrained and unconstrained configuration, respectively, and explore whether weight-correction increases their utility. Accordingly, the mid-shaft of the proximal half of femurs from OVX and sham-operated (Sham) mice was methacrylate-anchored and the head loaded parallel to the diaphysis (constrained). Alternatively, femurs from OVX and Sham rats were isolated intact and axially-loaded through hip and knee joint articular surfaces (unconstrained). Yield displacement, stiffness, maximum load and resilience were measured and fracture pattern classified; effects of body weight-correction via a linear regression method or simple division were assessed. Our data reveal significant deficiencies in biomechanical properties in OVX mouse femurs loaded in constrained configuration, only after weight-correction by linear regression. In addition, evaluation of rat femur biomechanics in unconstrained loading demonstrated greater variation and that weight-correction by simple division improved scope to reveal significant OVX impact. We conclude that greater femoral neck fracture susceptibility can indeed be measured in OVX rodents as long as multiple biomechanical parameters are reported, care is taken in choosing the method for assessing load-bearing strength and weight-correction applied. These studies advance the establishment of more relevant rodent models for the study of femoral neck fracture.

Peri-implant bone resorption risk of anterior maxilla narrow single implants: a finite-element analysis.

Statement of the problem: Narrow implants have been recommended in high esthetic demand regions to ensure greater buccal bone thickness (BBT) and minimize soft-tissue recession due to insufficient bone support. However, a limited area of bone-implant interface can increase the risk of peri-implant bone resorption due to occlusal forces. Purpose: This article encourages the use of evidence-based finite element analysis to optimize the aesthetic outcomes in maxillary lateral incisor single-supported implant crown by accurate biomechanical planning. This study aimed to analyze the best implant dimensions that would preserve the maximum BBT and avoid peri-implant bone resorption due to occlusal forces. Materials and methods: A maxilla segment was constructed based on anthropological measurements. Four implant diameters (Ø = 3.25; 3.50; 3.75 or 4.00 mm) and two lengths (L = 10 or 13 mm) were simulated. The occlusal force parameters were defined to simulate clinical conditions. The bone resorption risk analysis was based on Frost's mechanostat theory altering the strain output to strain energy density (SED). The peri-implant bone resorption risk indexes (PIBRri) were calculated by dividing the average of the top ten SED elements of the cortical and trabecular buccal wall by the pathologic resorption limit for each bone. Results: For trabecular bone, only the model Ø4.00L13 exhibited a low PIBRri. For cortical bone, all models presented a low PIBRri, except for models Ø3.25. Conclusion: The selection of a 3.25 mm dental implant to preserve a 2 mm BBT should be avoided since it generates a high peri-implant bone resorption risk induced by occlusal overload.

Comparation of three methods for measuring the Edge Bevel Radius of rectangular orthodontic wires: An in-vitro study.

OBJECTIVE: To establish a simplified method for measuring the edge bevel radius of 0.019×0.025-in steel rectangular orthodontic wires, achievable in the clinical environment, and to compare it with the photographic method. MATERIALS AND METHODS: Aided by the AutoCAD™ 2016 software, the theoretical mathematical relationship was determined between the edge rounding radius and cross-sectional dimensions (height, width, diagonals), through rectangle drawings that represent cross-sections. Two hundred segments (n=20) were obtained from the posterior portions of 100 archwires from 10 brands (Dentaurum™; American-Orthodontics™; GAC™; 3M-Unitek™; Abzil™; Morelli™; Orthometric™; Aditek™; A-Company™; Orthomundi™). The cross-sectional measures of each segment were obtained with a micrometer and a caliper, and the edge bevel radii of these segments were calculated with the use of a mathematical formula. For the "gold standard", the segments were included longitudinally in a phenolic resin cylinder, cross-sectioned, polished, and photographed in scanning electron microscope (SEM). The images were amplified 770× and the radii were measured with an acetate template. The micrometer and caliper measuring methods were subjected to Bland-Altman analysis and compared with the gold standard (SEM), considering ±10µm as the maximum acceptable difference between methods. RESULTS: In the micrometre measurement, 95% of the differences from the standard were within the limits of agreement (-7.21 and 5.56µm). For the caliper method, 95% of the differences were between -5.46 and 19.83µm, which exceeded the fixed limit ±10µm. CONCLUSION: The calculation method with micrometre measurements is equivalent to the photographic method, but there is no such equivalence for the caliper measurements.