RESUMO
OBJECTIVE: Ionic liquid based microwave-assisted extraction (ILMAE) was developed for the effective extraction of five anthraquinones namely aloeemodin, rhein, emodin, chrysophanol and physcion from Polygonum cuspidatum. METHODS: Experimental factors and levels were firstly selected by one-factor test. According to the central composite experimental design principle, the response surface methodology with three factors and five levels was adopted for comprehensive assessment by total extraction yield of five anthraquinones as a target. RESULTS: The optimized parameters of MAE were: 0.6 mol/L [C8 MIM] Br, solvent to solid ratio of 40:1, power of 200 W, temperature of 31 degrees C and extraction time of 8 min. Under the optimal conditions, this approach gained total extraction yield of five anthraquinones 11.69 mg/g, while the predicted total extraction yield was 11.74 mg/g. CONCLUSION: The research indicates ILMAE is an efficient, rapid, environment friendly and simple sample preparation technique to five anthraquinones from Polygonum cuspidatum.
Assuntos
Antraquinonas/isolamento & purificação , Medicamentos de Ervas Chinesas/isolamento & purificação , Fallopia japonica/química , Micro-Ondas , Antraquinonas/química , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas/química , Emodina/análogos & derivados , Emodina/isolamento & purificação , Líquidos Iônicos/química , Raízes de Plantas/química , Rizoma/química , Tecnologia Farmacêutica , TemperaturaRESUMO
OBJECTIVE: To analyze the floral volatiles of Polyonum cuspidatum flower. METHODS: The floral volatiles of P. cuspidaturn flower were investigated by Headspace Sampler GC/MS. RESULTS: 21 compounds were separated and determined from P. cuspidatumrn, which amounted to 99.29% of the total volatiles. 5 compounds including (E)-2-hyexenoic acid methyl ester, 1-phenyl-l-pentanone, (E)-4-hexenoic acid methyl ester, 3-hexenoic acid methyl ester, 2-methyl-6-methylene-1 and 7-octadien-3-one were the main constituents existed in P. cuspidatum flower, which amounted to 63.23% of the total volatiles. Organic ester compounds amounted to 52.09% of the total volatiles. Hexenoic acid methyl ester compounds were most, which amounted to 85.66% of the total organic ester compounds. In addition, the 1-phenyl-1-pentanone and acetophenone were 16.30% and 4.33%, which amounted to 20.63% of the total volatiles. CONCLUSION: The result of this research provides a scientific method for the reasonable exploitation and utilization of P. cuspidatum flower.