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1.
Zhongguo Zhong Yao Za Zhi ; 41(2): 197-204, 2016 Jan.
Artigo em Zh | MEDLINE | ID: mdl-28861963

RESUMO

The transplants of the two-year-old Glycyrrhiza uralensis were subjected to four concentration of brassinolide (BR 0.1, 0.4, 0.7, 1.0 mg•L⁻¹) in July. The morphological characters ( plant height, stem diameter, nodes number, internode length and root length , root thick, root fresh weight and root dry weight ) were measured and seven kinds of chemical constituents (glycyrrhizic acid, liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin, liquiritin apioside, isoliquiritin apioside) were determined by HPLC with the aim of increasing sinter output and improving quality of G. uralensis. Then the long-term dynamic changes of these morphological characters and chemical compositions' content were analyzed. The results showed that morphological characters of plant height, stem diameter, root length , root thick, root fresh weight and root dry weight increased remarkably with the 0.7 mg•L⁻¹ BR stimulating 2 months later,the increase rates were: 15.09%,6.15%,16.52%,8.46%,21.90%,29.41%, respectively. The content of glycyrrhizic acid, liquiritin, isoliquiritin, liquiritigenin, liquiritin apioside, isoliquiritin apioside were increased 20.16%,45.31%,53.56%,27.66%,23.54%,8.46% with the 0.7 mg•L⁻¹ BR stimulating 2 months later. The best effects were achieved in 2 months after brassinolide stimulating. The conclusions prove that morphological characters and the main chemical constituents accumulation of G. uralensis could be effected by exogenous BR stimulation in certain case.


Assuntos
Brassinosteroides/farmacologia , Medicamentos de Ervas Chinesas/análise , Glycyrrhiza uralensis/efeitos dos fármacos , Glycyrrhiza uralensis/crescimento & desenvolvimento , Reguladores de Crescimento de Plantas/farmacologia , Esteroides Heterocíclicos/farmacologia , Brassinosteroides/análise , Flavanonas/análise , Flavanonas/metabolismo , Glucosídeos/análise , Glucosídeos/metabolismo , Glycyrrhiza uralensis/química , Glycyrrhiza uralensis/metabolismo , Ácido Glicirrízico/análise , Ácido Glicirrízico/metabolismo , Reguladores de Crescimento de Plantas/análise , Raízes de Plantas/química , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/metabolismo , Esteroides Heterocíclicos/análise
2.
Yao Xue Xue Bao ; 50(7): 906-9, 2015 Jul.
Artigo em Zh | MEDLINE | ID: mdl-26552155

RESUMO

ß-Amyrin synthase (ß-AS) genes of Glycyrrhiza uralensis from 6 different regions were analyzed by PCR-SSCP and sequenced, then the correlationship between ß-AS SNP and regions of Glycyrrhiza uralensis were determined. According to the 1 coding single nucleotide polymorphism on the first exon of ß-AS gene at 94 bp site, Glycyrrhiza uralensis could be divided into 3 genotypes. In these genotypes, the percentage of 94A type in genuine regions was much higher, and it had significant differences with the percentage in non-genuine regions (P < 0.001). The results of the experiment proved that different ß-AS genotypes at 94 bp site from different regions may be one of the important reasons to result in the genuineness of Glycyrrhiza uralensis.


Assuntos
Genótipo , Glycyrrhiza uralensis/genética , Transferases Intramoleculares/genética , Proteínas de Plantas/genética , Éxons , Glycyrrhiza uralensis/classificação , Glycyrrhiza uralensis/enzimologia , Polimorfismo de Nucleotídeo Único , Polimorfismo Conformacional de Fita Simples
3.
Zhongguo Zhong Yao Za Zhi ; 39(24): 4759-62, 2014 Dec.
Artigo em Zh | MEDLINE | ID: mdl-25898573

RESUMO

The fruit of Lycium ruthenicum is a common folk medicine in China. Now it is popular for its antioxidative effect and other medical functions. The adulterants of the herb confuse consumers. In order to identify a new adulterant of L. ruthenicum, a research was performed based on NCBI Nucleotide Database ITS Sequence, combined analysis of the origin and morphology of the adulterant to traceable varieties. Total genomic DNA was isolated from the materials, and nuclear DNA ITS sequences were amplified and sequenced; DNA fragments were collated and matched by using ContingExpress. Similarity identification of BLAST analysis was performed. Besides, the distribution of plant origin and morphology were considered to further identification and verification. Families and genera were identified by molecular identification method. The adulterant was identified as plant belonging to Berberis. Origin analysis narrowed the range of sample identification. Seven different kinds of plants in Berberis were potential sources of the sample. Adulterants variety was traced by morphological analysis. The united molecular identification-origin-morphology research proves to be a preceding way to medical herbs traceability with time-saving and economic advantages and the results showed the new adulterant of L. ruthenicum was B. kaschgarica. The main differences between B. kaschgarica and L. ruthenicum are as follows: in terms of the traits, the surface of B. kaschgarica is smooth and crispy, and that of L. ruthenicum is shrinkage, solid and hard. In microscopic characteristics, epicarp cells of B. aschgarica thickening like a string of beads, stone cells as the rectangle, and the stone cell walls of L. ruthenicum is wavy, obvious grain layer. In molecular sequences, the length of ITS sequence of B. kaschgarica is 606 bp, L. ruthenicum is 654 bp, the similarity of the two sequences is 53.32%.


Assuntos
Berberis/classificação , Código de Barras de DNA Taxonômico/métodos , Medicamentos de Ervas Chinesas/isolamento & purificação , Lycium/classificação , Berberis/citologia , Berberis/genética , China , DNA de Plantas/química , DNA de Plantas/genética , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/genética , Contaminação de Medicamentos , Medicamentos de Ervas Chinesas/normas , Lycium/citologia , Lycium/genética , Medicina Tradicional Chinesa , Filogenia , Análise de Sequência de DNA , Especificidade da Espécie
4.
Chin J Nat Med ; 14(3): 236-40, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27025372

RESUMO

This study aimed to trace sources and quantitatively analyze the specnuezhenide content of circular Fructus Ligustri Lucidi for clinical use. Different specifications of Fructus Ligustri Lucidi were identified using DNA barcoding technology and the specnuezhenide content was analyzed by High Performance Liquid Chromatography (HPLC). The ITS sequence of circular Fructus Ligustri Lucidi was identical to that of standard privet, which was determined through botanical identification. ITS sequence similarity between circular Fructus Ligustri Lucidi and Fructus Ligustri Lucidi which was registered in NCBI ranged from 99.5% to 100%. The sequences of circular and other Fructus Ligustri Lucidi were clustered in a Neighbor-Joining tree with bootstrap value of 95, and these sequences could be distinguished from adulterants. Conforming to pharmacopoeia standard, the average specnuezhenide content of circular Fructus Ligustri Lucidi was higher than that of chicken waist Fructus Ligustri Lucidi. Circular Fructus Ligustri Lucidi derived from Ligustrum lucidum Ait. and the specnuezhenide content was higher in circular Fructus Ligustri Lucidi than that in chicken waist Fructus Ligustri Lucidi.


Assuntos
Ligustrum/classificação , Cromatografia Líquida de Alta Pressão , Código de Barras de DNA Taxonômico , DNA de Plantas , Frutas/química , Glucosídeos/isolamento & purificação , Ligustrum/química , Ligustrum/genética , Medicina Tradicional Chinesa , Reação em Cadeia da Polimerase , Piranos/isolamento & purificação , Controle de Qualidade
5.
Rev. bras. farmacogn ; 26(4): 490-496, July-Aug. 2016. tab, graf
Artigo em Inglês | LILACS | ID: lil-792711

RESUMO

ABSTRACT One-year-old Glycyrrhiza uralensis Fisch. ex DC, Fabaceae, was treated with three exogenous phytohormones in June and July, namely gibberellin, auxin (indole-3-acetic acid), methyl jasmonate at different concentrations. Control plants were treated with water. Roots of controls and hormones-treated G. uralensis plants were harvested at different times, and the contents of seven main chemical components were determined. Root glycyrrhizic acid content of plants treated in June increased significantly compared with controls, and the difference was significant. As for plants treated in July, root glycyrrhizic acid content increased in which sprayed with appropriate concentrations of hormones, but the effects of hormones were more evident in plants treated in June coincided with the vigorous growth period than those treated in July. Gibberellin at 40 mg/l and auxin at 40 mg/l applied in the two treatment periods significantly promoted the accumulation of glycyrrhizic acid in G. uralensis root. Treatment with methyl jasmonate at 100 and 25 mg/l in June and July, respectively, also increased glycyrrhizic acid content significantly. The determination of major active compositions indicated that liquiritin, isoliquiritin, isoliquiritin apioside and liquiritin apioside contents were positively related to glycyrrhizic acid content. The study preliminarily found phytohormones and the main chemical components associated with glycyrrhizic acid content, and these discoveries could provide a basis for establishing a chemical control network with glycyrrhizic acid as the core, confirming the secondary product metabolic pathways in the network and completely uncovering synthesis mechanism underlying glycyrrhizic acid-combined functional gene polymorphism.

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