[The isolation and study of the properties of current influenza A viruses (H1N1) with a natural resistance to remantadine]. / Vydelenie i izuchenie svoistv sovremennykh virusov grippa A (H1N1) s prirodnoi rezistentnost'iu k remantadinu.

Studies of influenza A viruses (H1N1) isolated in 1985-1988 revealed 4 strains with natural resistance to chemical drugs (remantadine, adapromine, deitiforin). Three of them were isolated in Mongolia (A/Mongolia/230/85, A/Mongolia/231/85, and A/Mongolia/128/86) and one in the USSR (A/Moscow/771/88). These strains differed from other isolates by the following features: (i) antigenic remoteness from reference A/Chile/1/83 and A/Taiwan/1/86; (ii) similarity in antigenic specificity with A/Khabarovsk/74/77; (iii) resistance to chemical drugs; (iv) high electrophoretic mobility of genes M and NS in PAG. The possible origin of these strains is discussed.

[The results of using the molecular hybridization of nucleic acids in the complex study of nasopharyngeal smears from patients with influenza and other acute respiratory diseases]. / Rezul'taty ispol'zovaniia molekuliarnoi gibridizatsii nukleinovykh kislot v kompleksnom issledovanii nosoglotochnykh smyvov ot bol'nykh grippom i drugimi ostrymi respiratornymi zabolevaniiami.

A complex study of samples obtained from patients with influenza and other acute respiratory diseases has revealed that the laboratory methods used in this study can be rated in the following order according to their sensitivity: isolation of the virus in chick embryos, analysis of seroconversions in the hemagglutination inhibition test, immunofluorescent determination of viral antigens, determination of viral antigens by enzyme immunoassay (EIA), detection of RNA-containing viral structures by means of molecular hybridization. From the point of view of the possibility of documenting influenza A in patients, the best results are achieved by the combination of molecular hybridization and EIA techniques: 90% and more of all cases. A rational scheme for the examination of samples obtained from patients with a view to epidemiological study, including both traditional and new rapid diagnostic methods, is proposed.