RESUMO
OBJECTIVE: This study was undertaken to evaluate erythrocyte membrane transport of L-arginine in pregnancy and immediately postpartum. STUDY DESIGN: The study comprised 103 women with normal pregnancy, initially evaluated at the second trimester (II), followed into the third trimester (III), and immediately postpartum (PP). Total erythrocyte L-arginine uptake was measured with (14)C-L-arginine, at 37 degrees C, for 3 minutes. The maximal transport capacity (V(max)) and half-saturation constant (K(m)) were obtained with the use of Michaelis-Menten kinetics. Results are expressed as mean+/-SD. Analysis of variance, followed by Tukey test, was used in statistical analysis (alpha< or =.05). RESULTS: V(max) (micromol/L cells per hour) progressively increased at each consecutive time period: 779+/-283, 946+/-289, and 1349+/-390, at II, III, and PP, respectively (P<.001). Similarly, K(m) (micromol/L) values increased from 56+/-20 at time II, to 62+/-18 at time III, and 69+/-24 at PP (P<.001). CONCLUSION: Total erythrocyte L-arginine uptake (V(max) and K(m)) increases progressively along normal pregnancy, with a further increase immediately postpartum.