RESUMO
The identification of 83 Corynebacterium, 13 Arcanobacterium haemolyticum, and 10 Rhodococcus equi strains by conventional methods (API Coryne complemented with 16S rRNA gene sequence analysis) was compared with matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry identification. The correlation between API and MALDI-TOF results was 89%. MALDI-TOF is a rapid and accurate system for identification of the above-mentioned microorganisms.
Assuntos
Arcanobacterium/isolamento & purificação , Técnicas Bacteriológicas/métodos , Corynebacterium/isolamento & purificação , Rhodococcus equi/isolamento & purificação , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Animais , Arcanobacterium/química , Arcanobacterium/classificação , Corynebacterium/química , Corynebacterium/classificação , Humanos , Rhodococcus equi/química , Rhodococcus equi/classificação , Sensibilidade e Especificidade , Fatores de TempoRESUMO
Bordetella bronchiseptica rarely infects immunocompetent humans. We report an unusual case of recurrent pertussis-like syndrome caused by B. bronchiseptica in a 7-month-old immunocompetent boy. Molecular analysis demonstrated that the isolates from the child and mother were identical.