The Arabidopsis RRM domain proteins EDM3 and IBM2 coordinate the floral transition and basal immune responses.

The transition from vegetative to reproductive development (floral transition) is a costly process in annual plants requiring increased investments in metabolic resources. The Arabidopsis thaliana (Arabidopsis) PHD finger protein EDM2 and RRM domain proteins EDM3 and IBM2 are known to form chromatin-associated complexes controlling transcript processing. We are reporting that distinct splice isoforms of EDM3 and IBM2 cooperate in the coordination of the floral transition with basal immune responses. These cooperating splice isoforms, termed EDM3L and IBM2L, control the intensity of basal immunity and, via a separate pathway, the timing of the floral transition. During the developmental phase prior to the floral transition expression of EDM3L and IBM2L strongly and gradually increases, while these isoforms simultaneously down-regulate expression of the floral suppressor gene FLC and promote the transition to reproductive growth. At the same time these accumulating EDM3 and IBM2 splice isoforms gradually suppress basal immunity against the virulent Noco2 isolate of the pathogenic oomycete Hyaloperonospora arabidopsidis and down-regulate expression of a set of defense-associated genes and immune receptor genes. We are providing clear evidence for a functional link between the floral transition and basal immunity in the annual plant Arabidopsis. Coordination of these two biological processes, which compete for metabolic resources, is likely critical for plant survival and reproductive success.

Comparison of bipolar membrane electrodialysis, electrodialysis metathesis, and bipolar membrane electrodialysis multifunction for the conversion of waste Na2SO4: Process performance and economic analysis.

To convert Na2SO4 into other high-value products (NaOH, H2SO4, and (NH4)2SO4), three types of cell configurations of electrodialysis (ED) were applied (three-compartment bipolar membrane ED (BMED), four-compartment ED metathesis (EDM) and five-compartment bipolar membrane ED multifunction (BMEDM)) and parameters such as average voltage variation, removal ratio of salt, product concentration, conversion rate, ion flux, and energy consumption were calculated and compared. The experimental results and calculations indicated that the overall performance of BMEDM was inferior to that of BMED and EDM. An industrial model was established, which indicated that the net profit from converting Na2SO4 using BMEDM was always higher than that from BMED and EDM. Based on the advantages of low investment (132 $) and energy cost (152 $/t Na2SO4), EDM was applicable to factories with a low output of Na2SO4 (production capacity <45%), whereas BMED (157.3 $/t Na2SO4) and BMED-5 (227.6 $/t Na2SO4) were applicable to factories with a high output of Na2SO4 (production capacity >45%) based on high net profits.

Computational Predictive and Electrochemical Detection of Metabolites (CP-EDM) of Piperine.

In this article, we introduce a proof-of-concept strategy, Computational Predictive and Electrochemical Detection of Metabolites (CP-EDM), to expedite the discovery of drug metabolites. The use of a bioactive natural product, piperine, that has a well-curated metabolite profile but an unpredictable computational metabolism (Biotransformer v3.0) was selected. We developed an electrochemical reaction to oxidize piperine into a range of metabolites, which were detected by LC-MS. A series of chemically plausible metabolites were predicted based on ion fragmentation patterns. These metabolites were docked into the active site of CYP3A4 using Autodock4.2. From the clustered low-energy profile of piperine in the active site, it can be inferred that the most likely metabolic position of piperine (based on intermolecular distances to the Fe-oxo active site) is the benzo[d][1,3]dioxole motif. The metabolic profile was confirmed by comparison with the literature, and the electrochemical reaction delivered plausible metabolites, vide infra, thus, demonstrating the power of the hyphenated technique of tandem electrochemical detection and computational evaluation of binding poses. Taken together, we outline a novel approach where diverse data sources are combined to predict and confirm a metabolic outcome for a bioactive structure.

The usefulness of lutein/trypan blue vital dye for the staining of corneal endothelium: a pilot study on DMEK pretreated tissues.

PURPOSE: The study aims to evaluate the usefulness of lutein/trypan blue vital dye for the staining of corneal tissues and endothelium-Descemet membrane (EDM) for Descemet membrane endothelial keratoplasty (DMEK). METHODS: Sixteen human corneal tissues (Eye Bank, Rome, Italy) were used. Corneal endothelium was tested at 25 s (T0), 1 min (T1), 2 min (T2), and 4 min (T4) from dye addition. Staining intensity and cell counting were compared. Stripped EDM was analyzed for selected apoptotic (AP, caspases, BCL2, BAX) and differentiation (VEGF-A, TGF-ß1RI, SMAD3/7, SMA) targets and changes in target expression. Protein extracts were analyzed through SDS-PAGE/IB. RESULTS: Although trypan blue staining produced the same color intensity of lutein/trypan blue dye in half the time, lutein/trypan blue reached a good and adequate color intensity at T4, which persisted even on excised and washed EDM grafts. Lutein/trypan blue-stained EDM showed a reduced number of blue-stained cells and AP immunoreactivity was significantly reduced in the same samples. An increased BCL2 transcript and a reduced BAX transcript were detected in lutein/trypan blue-stained EDM. No significant changes were observed for the main effector caspases (3/9) upon both treatments and the target genes representative of endothelial cell trans-differentiation (TGF-ß1RI, SMAD3/7, SMA). A trend in vascular endothelial growth factor (VEGF-A) regulation was observed in lutein/trypan blue-treated EDM grafts. CONCLUSION: Obtained results suggest that lutein/trypan blue dye deserves attention in the DMEK field and support the potential routine use of this dye as a valid alternative to trypan blue for all procedures devoted to the assessment of endothelial cell viability and visualization of EDM graft before DMEK grafting.

COVID-19-Related Shifts in Polysubstance Use.

Background: Big Events, such as economic crises and natural disasters, affect drug use patterns (e.g. Friedman & Rossi, 2015). The COVID-19 pandemic is a Big Event that led to lockdowns, travel restrictions, protocols on businesses, and rules for social engagements across the globe. Studies primarily in Europe and Oceania show that the pandemic impacted the type and amount of substances used (e.g. Winstock et al., 2020). Objectives: This study examines the impact of COVID-19 on substance use using a sample of 257 individuals across 36 states, who engage in polysubstance use. Results: The sample was recruited via DanceSafe, Inc.'s social media to complete an online survey (April-October 2020) about drug use during the pandemic. The mostly White, heterosexual sample used an average of seven different substances in the past 12 months. Slightly less than half reported increasing use since the onset of the COVID-19 pandemic, with young adults and lesbian, gay, bisexual, pansexual, or queer (LGBPQ) identifying individuals significantly more likely to do so. Relative to other substances, benzodiazepine use increased, and 3,4- methylenedioxymethamphetamine (MDMA) and psychedelic use decreased, while alcohol use stayed the same. Conclusions: The COVID-19 pandemic disproportionately affected those who are young adults, LGBPQ, and use drugs. Their unique needs during the pandemic warrant attention. The swap from leisure (e.g. MDMA) to anti-anxiety (e.g. Xanax) drugs is not surprising. Yet, the rise in novel benzodiazepines (Laing et al., 2021) is a point of concern that suggests drug checking and educational efforts can best reduce potential risks.

Display of WEDM Quality Indicators of Heat-Resistant Alloy Processing in Acoustic Emission Parameters.

The widespread nature of heat-resistant alloys is associated with the difficulties in their mechanical machining. It forces the use of the wire electrical discharge machining to be wider. The productivity, roughness, and dimensions of the modified layer of the machined surfaces are indicators of the machining quality. The search for new diagnostic parameters that can expand the information content of the operational monitoring/diagnostics of wire electrical discharge machining and accompany the currently used electrical parameters' data is an urgent research task. The article presents the studies of the relationship between the parameters of acoustic emission signals accompanying wire electrical discharge machining of heat-resistant alloys, process quality indicators, and characteristics of discharge pulses. The results are presented as mathematical expressions and graphs demonstrating the experimentally obtained dependencies. The research focuses on the formed white layer during wire electrical discharge machining. Pictures of thin cross-sections of the machined surfaces with traces of the modified layer are provided. The issues of crack formation in the modified layer and base materials are considered.

Investigation of the efficacy of different Ni-Ti systems on acrylic blocks for correcting ledge formation.

BACKGROUND: The aim of this study was to compare the efficacy of K-type stainless steel hand instruments (Mani Inc. ), Fanta AF™ Ledge Correction (LC) (Fanta Dental), and Hyflex EDM (Coltene-Whaledent) for ledge correction, canal transport, centric ability, and shaping (preparation) time after an artificial ledge has been bypassed manually in highly curved canals using acrylic blocks. METHODS: Forty-two resin blocks, each with a radius of 5 mm (Endo Trainer Block, VDW) and an apical inclination of 55°, were used. Under stereomicroscope magnification, standard artificial ledges were created on acrylic blocks, and attempts were then made to eliminate them using hand instruments, FantaAF™ LC, and Hyflex EDM. Before and after images were obtained using a stereomicroscope and compared using Photoshop. RESULTS: Fanta AF™ LC and Hyflex EDM were found to be more effective for correcting ledges than hand instruments. The use of hand instruments resulted in the greatest transportation away from the canal curvature in the apical area. The canal shaping was completed in the shortest amount of time using Fanta AF™ LC, followed by HyFlex EDM and then the hand instruments. CONCLUSION: In terms of centric ability, the order from best to worst is as follows: Fanta AF™ LC, Hyflex EDM, and hand instruments. After the ledge was manually bypassed with hand instruments in the root canals, Hyflex EDM and Fanta AF™ LC were found to be more effective than hand instruments in reshaping the previously unreachable region between the ledge and the foramen apical.

In vitro evaluation of filling material removal and apical debris extrusion after retreatment using Reciproc blue, Hyflex EDM and ProTaper retreatment files.

OBJECTIVES: To evaluate the amount of remaining filing material and apical debris extrusion after retreatment using Reciproc Blue, Hyflex EDM and ProTaper Retreatment Files. MATERIALS AND METHODS: Thirty-six extracted permanent mandibular first molars with moderately curved mesial roots were selected. Mesiobuccal canals were prepared using the ProTaper Next system up to size X2 and filled using gutta-percha and Adseal sealer via cold lateral compaction. Teeth were randomly divided into three equal groups (n = 12): Group 1: Reciproc Blue (RB)(VDW, Germany), Group 2: Hyflex EDM (HEDM)(Coltene/Whaledent, Switzerland), Group 3: ProTaper Universal Retreatment file system + ProTaper Next file system (PTUR + PTN)( Dentsply Maillefer, Switzerland). Eppendorf tubes were used to collect the apically extruded debris. Cone-beam computed tomographic scans were taken prior to and after retreatment and the volume of remaining filling material was assessed at the coronal, middle and apical levels. Statistical analysis was performed using the Kruskal-Wallis test, Friedman's test and Wilcoxon Sign Rank test. Significance level was set at p value 0.05. RESULTS: There were no statistically significant differences among the three groups in the reduction of the volume of the filling material or in the amount of apically extruded debris. CONCLUSION: All the tested filing systems showed similar efficacy in removing the filling material, however, none of them could achieve its complete removal. Apical extrusion of debris occurred with all the systems used with no significant difference between the three groups.

Randomized Controlled Trial of a Remote Coaching mHealth Adherence Intervention in Youth Living with HIV.

Youth living with HIV (YLWH) in the US have low rates of viral suppression (VS). In a prospective randomized clinical trial (ATN152) that enrolled 89 YLWH on antiretroviral therapy (ART) with detectable viral load, we evaluated a 12 week triggered escalating real-time adherence (TERA) intervention with remote coaching, electronic dose monitoring (EDM), and outreach for missed/delayed doses compared to standard of care (SOC). Median [Q1, Q3] percent days with EDM opening was higher in TERA (72% (47%, 89%)) versus SOC (41% (21%, 59%); p < 0.001) and incidence of numbers of 7 day gaps between openings were lower (TERA to SOC ratio: 0.40; 95% CI 0.30, 0.53; p < 0.001). There were no differences in VS at week 12 (TERA 35%; 95% CI 21%, 51% versus SOC 36%; 95% CI 22%, 51%; p > 0.99) or later time-points. The intervention improved adherence but not VS in heavily ART-experienced YLWH. Remote coaching more closely tailored to the unique dosing patterns and duration of need for youth struggling to reach VS warrants further investigation.

Ns-pulsewidth pulsed power supply by regulating electrical parameters for AFM nano EDM of nm-removal-resolution.

Nano electro discharge machining (nano EDM), as a frontier processing method in the research stage of exploration, has an important application prospect in the machining of metal and alloy materials for achieving nanoscale removal resolution. A pulsed power supply used in nano EDM is expected to limit a single pulse energy to nJ order of magnitude for improving the removal resolution of single pulsed discharge even to nanoscale. One developing direction is to decrease pulsewidth of the pulsed power supply. Conventional pulsed power supplies hardly output a single pulse and continuous pulses with nanosecond (ns) pulsewidth, resulting in too large single pulsed energy ofµJ order of magnitude usually. In this research, a novel pulsed power supply is designed for realizing the ns-pulsewidth with controllable pulsewidth and peak voltage. The key novelty lies in a cascaded circuit with two triodes working in the state of ultra-fast avalanche conduction, where pF capacitors are applied to adjust the pulsewidth and pulsed energy precisely. Performance tests verified that a single pulse of 5 ns pulsewidth or continuous pulses up to 9 MHz can be outputted. Furthermore, nano EDM experiments of single pulsed discharge are carried out under the conditions of nanometer (nm) discharge gap and nm-tip tool electrode based on an atomic force microscope (AFM) system. The special results are achieved: a single pulsed energy can reach down to 1.75 nJ by outputting a pulsewidth of 10 ns, and a nano-EDM crater is only about 182 nm in diameter with regular shape and little recasting. Those results verify the possibility of AFM-tip-based nano EDM for machining nanostructures.

Glide path enlargement of curved molar canals using HyFlex EDM glide path file versus PathFile: a comparative study of preparation time and postoperative pain.

BACKGROUND: This randomized clinical trial aimed to compare the preparation time and severity of postoperative pain between HyFlex Electric Discharge Machine (EDM) glide path file (GPF) and PathFile. METHODS: Eighty patients whose molar teeth had at least one severely curved canal were treated by the same specialist. After access cavity preparation, the patients were randomly assigned to receive glide path enlargement with either HyFlex EDM GPF or PathFile. ProTaper Next X1 and X2 files were used to prepare the canals. The time of preparation was assessed and the severity of postoperative pain over the next 7 days was recorded. The preparation time and the postoperative pain scores were compared using the Linear Mixed Models (P ≤ 0.05). RESULTS: Glide path enlargement time was significantly shorter with HyFlex EDM GPF (27.828 ± 2.345 s) than with PathFile (48.942 ± 2.864 s) (P < 0.001). The highest postoperative pain score was recorded on the first day and the pain decreased over time in both groups. HyFlex EDM GPF group patients reported significantly less postoperative pain than PathFile group patients overall (P < 0.001). CONCLUSIONS: Postoperative pain and glide path preparation time could be reduced by using HyFlex EDM GPF system. Trial registration PKUSSNCT, PKUSSNCT-17B12, Registered 24 October 2017.

Comparison of Canal Transportation in TruNatomy, ProTaper Gold, and Hyflex Electric Discharge Machining File Using Cone-beam Computed Tomography.

AIM: This study was carried out to compare canal transportation in three new rotary file systems, namely TruNatomy, ProTaper Gold, and Hyflex Electric Discharge Machining file system, using cone-beam computed tomography. MATERIALS AND METHODS: The study was conducted on 105 extracted teeth consisting of 60 extracted mandibular first molars and 45 mandibular second premolars involving the mesiolingual canal of mandibular first molar and the root canal of second premolar. The teeth were randomly divided into three groups of 35 out of which each group consisted of 20 mandibular first molars and 15 mandibular second premolars. The TruNatomy rotary file was used in group I, ProTaper Gold was used in group II, and Hyflex Electric Discharge Machining was used in group III. Cone-beam computed tomography scan images were obtained both before and after instrumentation. Changes caused by preparation in the coronal, middle, and apical thirds were determined on cone-beam computed tomography scans and analyzed using the Kruskal-Wallis test at p ≤ 0.05 level of significance. RESULTS: TruNatomy showed least amount of canal transportation as compared with other two file systems at all the three levels of canals. ProTaper Gold showed maximum amount of canal transportation as compared with other two file systems at all the three levels of canals. The Hyflex EDM rotary file system showed transportation, which was more than the TruNatomy file system while it was less than that of ProTaper Gold. The difference was statically significant at the middle one-third level (p = 0.03) and at the coronal level (p = 0.02). CONCLUSION: The TruNatomy system has least amount of canal transportation as compared with ProTaper Gold and Hyflex EDM system. With potential to preserve tooth structure, this file has an added advantage over other rotary file systems. CLINICAL SIGNIFICANCE: The TruNatomy file system has been recently introduced with good cyclic fatigue resistance. It is necessary to evaluate the root canal transportation caused by the TruNatomy file.

Comparison of Cyclic Fatigue Resistance of Novel TruNatomy Files with Conventional Endodontic Files: An In Vitro SEM Study.

AIM AND OBJECTIVE: To compare and analyze the cyclic fatigue resistance of novel TruNatomy files against the conventionally used rotary files and reciprocating files in a simulated canal system with curvatures in the middle, apical portions root, and S-shaped canal employing scanning electron microscopy. MATERIALS AND METHODS: Endodontic files tested in the study include ProTaper, HyFlex EDM, Reciproc blue, and TruNatomy files operated in a simulated root canal system. A total of 96 files were employed in this study. They were categorized into four groups. Group 1 employed 24 TruNatomy files, group 2 employed 24 ProTaper gold files, group 3 employed 24 HyFlex EDM files, and group 4 employed 24 Reciproc blue files. All the groups employed eight files each under three subgroups namely A, B, and C representing middle, apical, and double curvatures, respectively. Artificial grooves were incorporated in the stainless steel blocks having three different curvatures. All the file systems were operated with a torque-controlled electric motor. The files were engaged in the artificial canal until the occurrence of fracture. The time required for the fracture was noted. Length of fractured tip was measured using the digital Vernier caliper. The number of cycles of failure (NCF) was calculated. The fractured parts were subjected to scanning electron microscope to analyze the mode of fracture. RESULTS: TruNatomy files had the highest mean NCF values of 588.01 in middle curvature, 1,321 in apical curvature, and 642.5 in S-shaped curvature HyFlex EDM and Reciproc blue file system exhibited comparable cyclic fatigue resistance among them. ProTaper gold file system exhibited least mean NCF values of 158.13 in the middle, 196.5 in the apical curvature, and 116.88 in the S-shaped canal system. Middle portion fractured first than the apical curvature. The mean length of fractured segments was found to be insignificant among the experimental study groups tested. CONCLUSION: All the file systems employed in the study had good cyclic fatigue resistance while TruNatomy files had the highest cyclic fatigue resistance while HyFlex EDM and Reciproc blue had comparable fatigue resistance. ProTaper gold file system had the least fatigue resistance at different radius of curvature in the simulated canal system in the apical and middle portions and S-shaped root canal. CLINICAL SIGNIFICANCE: The selection of file systems in cleaning and shaping protocols is an enigma to the endodontics. This in vitro study explored the selection protocols for the execution of root canal preparation. Heat treatment of nickel-titanium (NiTi) endodontic files had improved the cyclic fatigue résistance significantly enhancing the clinical life of file systems.

The effect of heat-treated single-file systems on dentinal crack formation.

OBJECTIVES: The aim of this study was to compare the formation of dentinal crack after root canal preparation performed with different heat treated single-file systems. MATERIALS AND METHODS: Forty mandibular premolar teeth were randomly divided into four groups according to the file system to be used during the preparation (n = 10): Group 1: Reciproc Blue; group 2: One Curve; group 3: HyFlex EDM; group 4: (control group) hand files. During the cleaning and shaping, irrigation was performed with a total of 10 ml of 5.25% sodium hypochlorite and 2 mL of distilled water. Then sections were taken from the roots (3, 6 and 9 mm). Sections were examined on a stereomicroscope to determine the presence of cracks. Chi-square test was used for data analysis. RESULTS: There was a significant difference between the groups in dentinal crack frequency (P <.05). HyFlex EDM (13.3%) caused less dentinal crack than One Curve (26.7%) and Reciproc Blue (30%). No cracks were observed in the control group. When 3, 6, and 9 mm were evaluated within themselves, dentinal crack formation did not differ according to the groups (P > 0.05). A statistically higher mean value was obtained in the control group compared to the other groups in terms of working time (P < 0.001). CONCLUSIONS: All the files used during root canal preparation formed dentin crack. Hy-Flex EDM caused less dentinal cracks than other file systems. The flexibility of nickel-titanium instruments because of heat treatment seems to have a significant influence on dentinal crack formation.

Clinical outcomes of patients with POLE mutated endometrioid endometrial cancer.

OBJECTIVES: Assess outcomes of a clinical cohort of patients with endometrioid endometrial cancer (EEC) harboring somatic POLE exonuclease domain mutations (EDMs). METHODS: Patients were consented to a protocol of tumor-normal massively parallel sequencing of 410-468 cancer related genes. EECs subjected to sequencing from 2014 to 2018 were reviewed. Tumors with somatic POLE EDMs were identified. EECs were assessed for microsatellite instability (MSI) using MSIsensor and immunohistochemical analysis for mismatch repair (MMR) proteins. RESULTS: Of the 451 EECs sequenced, 23 had a POLE EDM (5%): 20 primary and 3 recurrent tumors sequenced. Nineteen cases (83%) were stage I/II and 4 (17%) were stages III/IV. Thirteen EECs (57%) were of FIGO grades 1/2, 10 (43%) grade 3. All patients were treated with surgery and 17 (89%) received adjuvant therapy. Five (22%) demonstrated loss of DNA MMR protein expression, none were due to Lynch syndrome. MSIsensor scores were conclusive for 21 samples: 19 were microsatellite stable and 2 MSI-high. After median follow-up of 30 months, 4/23 (17%) developed recurrences: 3 with initial grade 3 stage I and 1 with grade 1 stage III disease. One patient with grade 2 stage IV EEC had progressive disease after treatment. CONCLUSIONS: Patients with POLE EDM EEC have been shown to have a favorable prognosis. In this real-world cohort of patients, de novo metastatic disease and recurrences in initially uterine-confined cases were observed. Further research is warranted before incorporating the presence of POLE EDM into decision-making regarding adjuvant therapy.

Temperature is a regulator of leaf production in the family Dipterocarpaceae of equatorial Southeast Asia.

PREMISE: Leaf phenology is an essential developmental process in trees and an important component in understanding climate change. However, little is known about the regulation of leaf phenology in tropical trees. METHODS: To understand the regulation by temperature of leaf phenology in tropical trees, we performed daily observations of leaf production under rainfall-independent conditions using saplings of Shorea leprosula and Neobalanocarpus heimii, both species of Dipterocarpaceae, a dominant tree family of Southeast Asia. We analyzed the time-series data obtained using empirical dynamic modeling (EDM) and conducted growth chamber experiments. RESULTS: Leaf production by dipterocarps fluctuated in the absence of fluctuation in rainfall, and the peaks of leaf production were more frequent than those of day length, suggesting that leaf production cannot be fully explained by these environmental factors, although they have been proposed as regulators of leaf phenology in dipterocarps. Instead, EDM suggested a causal relationship between temperature and leaf production in dipterocarps. Leaf production by N. heimii saplings in chambers significantly increased when temperature was increased after long-term low-temperature treatment. This increase in leaf production was observed even when only nighttime temperature was elevated, suggesting that the effect of temperature on development is not mediated by photosynthesis. CONCLUSIONS: Because seasonal variation in temperature in the tropics is small, effects on leaf phenology have been overlooked. However, our results suggest that temperature is a regulator of leaf phenology in dipterocarps. This information will contribute to better understanding of the effects of climate change in the tropics.

Comparison of the cyclic fatigue resistance of hyflex EDM, vortex blue, protaper gold, and onecurve nickel-Titanium instruments.

OBJECTIVE: This in vitro study aimed to compare the cyclic fatigue resistance of HyFlex EDM (HEDM), Vortex Blue (VB), ProTaper Gold (PG), and OneCurve (OC) nickel-titanium (NiTi) instruments. MATERIALS AND METHODS: About 12 HEDM (25/.~), 12 VB (25/.06), 12 PG (25/.08), and 12°C (25/.06) instruments were included in this study. All the instruments were tested with a 60° angle of curvature and a 3-mm radius of curvature. All the instruments were utilized until fracture occurred, and then the number of cycles to failure (NCF) was calculated. The data were analyzed statistically using Kruskal-Wallis H and Mann-Whitney U-tests. The statistical significance level was set at P < 0.05. RESULTS: HEDM instruments had the highest cyclic fatigue resistance among all the other instruments (P < 0.05). The OC instruments had a significantly higher fatigue resistance than the PG and VB instruments (P < 0.05); however, there was no significant difference between PG and VB instruments in the NCF (P > 0.05). CONCLUSION: Within the limitations of this in vitro study, it was found that the cyclic fatigue resistance was higher for the HEDM instruments than for the VB, PG, and OC instruments.

Demethylation of ERECTA receptor genes by IBM1 histone demethylase affects stomatal development.

DNA methylation and histone modifications interact to modulate gene expression in biological organisms. The histone demethylase IBM1 suppresses DNA methylation and gene silencing, primarily by targeting genic regions in the Arabidopsis genome. The chromatin regulator EDM2 is also required for prevention of genic DNA methylation because it maintains IBM1 expression by promoting IBM1 mRNA distal polyadenylation. Loss-of-function ibm1 and edm2 mutant plants display a wide range of developmental defects, but little is known about which developmentally important genes are regulated by IBM1 and EDM2. Here, we show that both ibm1 and edm2 mutants display defects in production of stomatal lineage cells, which is linked to DNA hypermethylation of the ERECTA family genes, including ER, ERL1 and ERL2 Stomatal phenotypes and DNA methylation levels of ER genes in ibm1 and edm2 mutants are restored by mutations in the genes encoding the histone methyltransferase KYP and DNA methyltransferase CMT3. Our data demonstrate that a specific plant developmental context is influenced by IBM1-regulated histone modification and DNA methylation on the gene body region of the ERECTA receptors.

Effects of etidronate, NaOCl, EDTA irrigation solutions and their combinations on cyclic fatigue resistance of nickel-titanium single-file rotary and reciprocating instruments at body temperature.

The aim of the present study was to compare the effect of etidronic acid (HEBP), NaOCl, and EDTA solutions and their combinations on cyclic fatigue resistance of NiTi RPC Blue (RPC Blue), HyFlex EDM (HEDM), and WaveOne Gold (WOG) instruments having different metallurgic characteristics at the body temperature (37 °C). 100 WOG, 100 RPC Blue, and 100 HEDM NiTi files were randomly divided into 5 groups and immersed in the following solutions: group 1, distilled water; group 2, 6% NaOCl; group 3, 17% EDTA; group 4, 18% HEBP; group 5, 6% NaOCl and 18% HEBP. Then the instruments were rotated in an artificial canal until fracture occurred and the number of cycles to fracture was calculated. A univariate analysis of variance and post hoc Tukey tests were then performed for comparisons using SPSS 21.0 software with the significance level set at P < .05. In all the conditions, there was no difference between the cyclic fatigue resistance of WOG and RPC Blue files (P > .05). In the intragroup comparison, WOG, RPC Blue, and HEDM files showed significantly low cyclic fatigue resistance in group 5 compared to other groups (P < .05). Among the groups, there was no statistically significant difference in the fracture lengths (P > .05). EDTA, NaOCl and HEBP solutions did not affect the cyclic fatigue resistance of the tested files when used without combination. However, the combination of NaOCl and HEBP solutions significantly reduced the cyclic fatigue resistance of the tested files.

Cyclic fatigue resistance of three rotary file systems in a dynamic model after immersion in sodium hypochlorite.

To evaluate the effect of immersion in 3% sodium hypochlorite solution in the resistance to cyclic fatigue of three nickel-titanium (NiTi) rotary file systems, ProTaper Next (PTN), Hyflex CM (CM), and Hyflex EDM (EDM), in a mechanical model featuring axial movement. Ninety instruments of three different NiTi rotary file systems, PTN (size 25, 0.06 taper), CM (25, 0.06), and EDM (25/~, variable taper), were randomly divided according to a 3 × 3 factorial design and tested under dynamic immersion in a 3% NaOCl solution (1 or 5 min) or without immersion, making a total of 9 groups (n = 10). Files were tested in an artificial root canal with 45° angle and 5 mm radius apical curvature being submitted to back-and-forth movements until fracture. Statistical analysis was performed using two-way factorial ANOVA with Bonferroni post-hoc tests, at a significance level of 5%. Instruments were evaluated for reliability using a Weilbull approach. Regardless of the immersion treatment, PTN had on average 1200 ± 178 cycles to fracture, CM had 1949 ± 362, and EDM had 5573 ± 853, which was a significantly different (P < 0.01). The NaOCl immersion promoted a significant reduction in the mean number of cycles to fracture (P = 0.01), and was reflected in a significant reduction of the characteristic life of the instruments of the CM end EDM groups. Within this study conditions, EDM instruments performed better to cyclic fatigue followed by CM and then PTN. Immersion in NaOCl decreased the resistance to cyclic fatigue of all tested instruments, but affected more those manufactured from CM wire.