1.
Methods Mol Biol
; 2752: 1-9, 2024.
Artigo
em Inglês
| MEDLINE
| ID: mdl-38194024
RESUMO
Primary cells form the basis of modern-day in vitro research analysis tools. Many conventional procedures for generating single-cell suspensions from solid tissue are neither robust nor reproducible. Here we describe primary cells isolation from surgically resected tumor tissue via enzyme-free mechanical dissociation using TissueGrinder, a novel semi-automated benchtop device. The isolated cells can be used for any downstream biochemical or cell-based analytic assay.