Anticandidal activity of Lactobacillus spp. in the presence of galactosyl polyols.

Yeasts have a substantial impact on the contamination and loss of food. In this study, we applied bacteria of the genus Lactobacillus as natural biopreservatives. Anticandidal strains of bacteria were selected from among 60 strains of bacteria grown which were each with nine polyols or galactosyl polyols. Polyols and galactosyl polyols can act as prebiotics for lactic acid bacteria and can enhance the antifungal properties of bacteria by affecting their metabolism. The galactosyl polyols significantly improved the anticandidal activity of most of the bacteria we tested. Based on the screening, the most promising strains of bacteria were selected, and their metabolites (both primary and secondary) and enzymatic activity were characterized in the presence of polyols and galactosyl polyols. The qualitative and quantitative content of bacterial metabolites depended both on the bacterial strain and the type of culture medium. A wide variety of antifungals produced by bacteria, such as fatty acids, hydroxy fatty acids, and other acidic products with potential antagonistic activity (phenyllactic acid or hydroxyphenyllactic acid) were detected. The bacteria produced a high concentration of phenyllactic acid in the presence of galactosyl polyols (up to 84.3 mg/L). This finding could suggest that this metabolite may have a significant impact on the antifungal properties of lactobacilli against yeast. Galactosyl polyols influenced the enzymes involved in the synthesis of fatty acids and hydroxylated fatty acids (esterase lipase, acid phosphatase, and α-glucosidase). The mechanism of the antifungal effect of lactobacilli may be based on the synergistic effect of their primary and secondary metabolites, in particular phenyllactic acid.

Antifungal Activity of Lactobacillus pentosus LOCK 0979 in the Presence of Polyols and Galactosyl-Polyols.

The antifungal activity of Lactobacillus pentosus LOCK 0979 depends both on the culture medium and on the fungal species. In the control medium, the strain exhibited limited antagonistic activity against indicator food-borne molds and yeasts. However, the supplementation of the bacterial culture medium with polyols (erythritol, lactitol, maltitol, mannitol, sorbitol, xylitol) or their galactosyl derivatives (gal-erythritol, gal-sorbitol, gal-xylitol) enhanced the antifungal properties of Lactobacillus pentosus LOCK 0979. Its metabolites were identified and quantified by enzymatic methods, HPLC, UHPLC-MS coupled with QuEChERS, and GC-MS. The presence of polyols and gal-polyols significantly affected the acid metabolite profile of the bacterial culture supernatant. In addition, lactitol and mannitol were used by bacteria as alternative carbon sources. A number of compounds with potential antifungal properties were identified, such as phenyllactic acid, hydroxyphenyllactic acid, and benzoic acid. Lactobacillus bacteria cultivated with mannitol synthesized hydroxy-fatty acids, including 2-hydroxy-4-methylpentanoic acid, a well-described antifungal agent. Scanning electron microscopy (SEM) and light microscopy confirmed a strong antifungal effect of L. pentosus LOCK 0979.