A biocompatible chitosan-based fluorescent polymer for efficient H2O2 detection in living cells and water samples.

As a biomarker of oxidative stress, hydrogen peroxide (H2O2) plays a complex role in organisms, including regulating cell signaling, respiration, the immune system, and other life processes. Therefore, it is important to develop a tool that can simply and effectively monitor H2O2 levels in organisms and the environment. In this work, naphthalene fluorophores with a borate structure were introduced into chitosan (CTS) azide, and a CTS-based fluorescence sensor (CTS-HP) was designed for sensitive H2O2 detection. The biocompatibility and degradability of CTS endowed CTS-HP with reduced biotoxicity compared with organic fluorescent dyes, and the substitution degree of fluorophores on the CTS chains was 0.703. The randomly coiled chain structure of the CTS-HP probe enabled the boronic acid recognition sites on the fluorophores to achieve the enrichment of analyte H2O2 through a synergistic effect. Therefore, the probe CTS-HP (10 µg mL-1) exhibited a 21-fold fluorescence enhancement and good detection limit (LOD = 8.98 nM) in H2O2 solution, reaching the maximum fluorescence response faster (within 16 min). The probe also successfully achieved the fluorescence imaging of endogenous and exogenous H2O2 in zebrafish and living cells and labeled the recovery experiment of H2O2 in real water samples (recoveries rates of 90.93-102.9 % and RSD < 3.09 %).

Triphenylamine-Based Polythioacetal for Selective Sensing of Mercury(II) with High Specificity and Sensitivity.

Utilizing the mercury (Hg2+)-triggered deprotection of thioacetals to aldehyde groups, we constructed a water-soluble triphenylamine (TPA)-based polythioacetal PTA-TPA with thioacetal groups in the backbones for efficient sensing of Hg2+ in aqueous solutions. PTA-TPA is conveniently prepared by polycondensation of 3, 6-dioxa-1,8-octanedithiol (DODT) with 4-(N,N-diphenylamino) benzaldehyde (TPA-CHO) using thiol-terminated mPEG2k-SH as a capping agent. The interaction of Hg2+ with PTA-TPA activates the aggregation-induced emission (AIE) process of TPA-CHO molecules, which makes the emission enhanced, and the emission color changes to sky blue, while other metal ions do not interfere with the sensing process. PTA-TPA can be used as a highly selective and ultrafast detection system for Hg2+ with a low detection limit (LOD) of 9.88 nM and a fast response of less than 1 min. In addition, the prepared test strips report the presence of Hg2+ with an LOD as low as 1 × 10-5 M. Intracellular imaging applications have demonstrated that PTA-TPA acts as a biocompatible fluorescent probe for efficient Hg2+ sensing in HeLa cells. Overall, the PTA-TPA fluorescence probes have the characteristics of easy synthesis, cost-effective, ultrafast detection speed, high selectivity, and high sensitivity, which can be used in practical applications.

Ultrafast and Efficient Detection of Formaldehyde in Aqueous Solutions Using Chitosan-based Fluorescent Polymers.

Detection of a toxic formaldehyde (HCHO) pollutant in aqueous solutions is of significant importance, because HCHO is widely found in aquatic food because of illicit addition or improper storage. Many small-molecule-based fluorescent probes, which rely on HCHO-specific formaldehyde-amine condensation or the aza-Cope rearrangement reaction, have been developed in terms of facile operation and high selectivity. However, some primary challenging issues are the restricted sensitivity and long equilibrium response time caused by the slow chemical reaction between these small-molecule-based sensors and low-concentration HCHO pollutant in testing samples. Herein, a robust hydrophilic hydrazino-naphthalimide-functionalized chitosan (HN-Chitosan)-based polymeric probe is reported, which takes advantage of specific chemical reaction between HCHO and grafted hydrazino-naphthalimide groups to trigger a "turn-on" fluorescence response. Superior to its small-molecule analogs, HN-Chitosan is based on random coil polymer chains of biopolymeric chitosan, which is thus capable of employing the cooperative binding effect of multiple hydrazino-naphthalimide recognition sites and adjacent hydroxyl groups to "enrich" the low-concentration HCHO pollutant around the polymer chains via weak supramolecular interactions. Therefore, the HCHO-specific chemical reaction with grafted hydrazino-naphthalimide groups is significantly accelerated, resulting in the unprecedented ultrafast equilibrium fluorescence response (less than 1 min) and high sensitivity. Encouraged by its satisfying sensitivity, selectivity, fast response, and wide linear detection range, we successfully expand its application to real-world food and water analysis. In view of the modular design principle of our polymeric probe, the proposed strategy could be generally applicable to construct powerful polymeric probes for ultrafast detection of other important pollutants.