Reducing biosolids from a membrane bioreactor system: Assessing the effects on carbon and nutrient removal, membrane fouling and greenhouse gas emissions.

This study presents the effects on carbon and nutrient removal, membrane fouling and greenhouse gas (GHG) emissions of an Oxic-Settling-Anaerobic (OSA) - Membrane Bioreactor (MBR) pilot plant fed with real wastewater. The influence of three sludge return internal ratios (IR) was investigated by testing 45, 75 and 100%. The results showed that with the increase of IR, the biological sludge production substantially decreased by 85.8% due to the combination of cell lysis and endogenous metabolism. However, a worsening of ammonia removal efficiencies occurred (from 94.5 % to 84.7 with an IR value of 45 and 100%, respectively) mostly due to the ammonia release caused by cell lysis under anaerobic conditions. The N2O emission factor increased with the rise of IR (namely, from 2.17% to 2.54% of the total influent nitrogen). In addition, a variation of carbon footprint (CF) (0.78, 0.62 and 0.75 kgCO2eq m-3 with 45, 75 and 100% IR, respectively) occurred with IR mainly due to the different energy consumption and carbon oxidation during the three periods. The study's relevance is to address the optimal operating conditions in view of reducing sludge production. In this light, the need to identify a trade-off between the advantages of reducing sludge production and the disadvantages of increasing membrane fouling and GHG emissions must be identified in the future.

Agronomic and phytotoxicity test with biosolids from anaerobic CO-DIGESTION with temperature and micro-organism phase separation, based on sewage sludge, vinasse and poultry manure.

This study deals with energy and agronomic valorisation by anaerobic co-digestion with temperature and microorganism phase separation of sewage sludge, vinasse and poultry manure, with the aim of achieving an integral waste management, obtaining bioenergy and biofertilizer that returns nutrients to the soil in a natural way. The yields obtained were 40 mL H2/gVS and 391 mLCH4/gVS. The resulting effluent showed more than 98 % removal of E. coli and Total Coliforms, as well as total removal of Salmonella. The results obtained in the phytotoxicity tests showed that all the proportions studied had phytostimulant and phytonutrient properties, with 20 % having the highest germination index (GI) with mean values of 145.30 %. Finally, the agronomic trial carried out with strawberry crops (Fragaria sp.) showed that the addition of this biosolid has fertilising properties and can be used as an agronomic amendment, with an increase of 145 % in fresh weight and 102.5 % in dry weight, and fruit production doubled with respect to the control. The ANOVA statistical study corroborated that there were significant differences in crop growth when applying different proportions of biofertilizer in the fertilizer. Therefore, these results show that this technology is promising and would contribute environmentally, socially and economically to the transfer towards a circular economy model.

Target and Suspect Screening Integrated with Machine Learning to Discover Per- and Polyfluoroalkyl Substance Source Fingerprints.

This study elucidates per- and polyfluoroalkyl substance (PFAS) fingerprints for specific PFAS source types. Ninety-two samples were collected from aqueous film-forming foam impacted groundwater (AFFF-GW), landfill leachate, biosolids leachate, municipal wastewater treatment plant effluent (WWTP), and wastewater effluent from the pulp and paper and power generation industries. High-resolution mass spectrometry operated with electrospray ionization in negative mode was used to quantify up to 50 target PFASs and screen and semi-quantify up to 2,266 suspect PFASs in each sample. Machine learning classifiers were used to identify PFASs that were diagnostic of each source type. Four C5-C7 perfluoroalkyl acids and one suspect PFAS (trihydrogen-substituted fluoroethernonanoic acid) were diagnostic of AFFF-GW. Two target PFASs (5:3 and 6:2 fluorotelomer carboxylic acids) and two suspect PFASs (4:2 fluorotelomer-thia-acetic acid and N-methylperfluoropropane sulfonamido acetic acid) were diagnostic of landfill leachate. Biosolids leachates were best classified along with landfill leachates and N-methyl and N-ethyl perfluorooctane sulfonamido acetic acid assisted in that classification. WWTP, pulp and paper, and power generation samples contained few target PFASs, but fipronil (a fluorinated insecticide) was diagnostic of WWTP samples. Our results provide PFAS fingerprints for known sources and identify target and suspect PFASs that can be used for source allocation.

Underestimation of Per- and Polyfluoroalkyl Substances in Biosolids: Precursor Transformation During Conventional Treatment.

Wastewater treatment plants generate a solid waste known as biosolids. The most common management option for biosolids is to beneficially reuse them as an agricultural amendment, but because of the risk of pathogen exposure, many regulatory bodies require pathogen reduction before biosolids reuse. Per- and polyfluoroalkyl substances (PFAS) are well documented in biosolids, but limited information is available on how biosolids treatment processes impact PFAS. Furthermore, quantification of PFAS has focused on perfluoroalkyl acids (PFAAs) which are a small fraction of thousands of PFAS known to exist. The objective of this study was to quantify 92 PFAS in biosolids collected from eight biosolids treatment facilities before and after four pathogen treatment applications: composting, heat treatment, lime treatment, and anaerobic digestion. Overall, total PFAS concentrations before and after treatment were dominated by PFAA precursor species, in particular, diPAPs which accounted for a majority of the mass of the Σ92PFAS. This differs from historic data that found PFAAs, primarily PFOS, to dominate total PFAS concentrations. Treatment options such as heat treatment and composting changed the ratio of PFAA precursors to PFAAs indicating a transformation of PFAS during treatment. This study finds that PFAA precursors are likely underrepresented by other studies and make up a larger percentage of the total PFAS concentration in biosolids than previously estimated.

Impacts of Environmental and Engineered Processes on the PFAS Fingerprint of Fluorotelomer-Based AFFF.

Forensic analysis can potentially be used to determine per- and polyfluoroalkyl substance (PFAS) sources at contaminated sites. However, fluorotelomer aqueous film-forming foam (AFFF) sources are difficult to identify because the polyfluorinated active ingredients do not have authentic standards and because the parent compounds can undergo transformation and differential transport, resulting in alteration of the PFAS distribution or fingerprint. In this study, we investigate changes in the PFAS fingerprint of fluorotelomer-derived AFFF due to environmental and engineered processes, including groundwater transport, surface water flow, and land application of contaminated biosolids. Fingerprint analysis supplemented by quantification of precursors and identification of suspected active ingredients shows a clear correlation between a fluorotelomer AFFF manufacturer and surface water of nearby Lake Michigan, demonstrating contamination (>100 ng/L PFOA) of the lake due to migration of an AFFF-impacted groundwater plume. In contrast, extensive processing during wastewater treatment and environmental transport results in large changes to the AFFF fingerprint near agricultural fields where contaminated biosolids were spread. At biosolids-impacted sites, the presence of active ingredients confirms contamination by fluorotelomer AFFF. While sediments can retain longer-chain PFAS, this study demonstrates that aqueous samples are most relevant for PFAS fingerprinting in complex sites, particularly where shorter-chain compounds have been used.

Investigating mixed biosolids and cardboard for methylene blue adsorption: Activation, adsorption modelling and thermodynamics.

Ongoing global population boom has led to the rise in waste and related research on increasing its economic value. In such an attempt, this study aims to activate gas-to-liquids (GTL) derived biosolids (BS) and cardboard (CB) and mixed samples (50:50) using potassium carbonate to produce three activated carbons (ACs): KBS, KCB and KM respectively. The characterization of the samples revealed surface areas of 156, 515, and 527 m2/g for KBS, KCB, and KM, respectively based on Brunauer-Emmett-Teller (BET) analysis, with increased porosity and metal content after activation evident from the Scanning Electron Microscopy-Energy Dispersive Spectroscopy (SEM-EDS) results, as well as the presence of magnetite in the KBS and KM samples apparent from the X-ray powder diffraction (XRD) results. Additionally, Fourier Transform Infrared Spectroscopy (FTIR) results indicate increased C-O-C stretches and O-H bonds after activation of the samples. The ACs were used for methylene blue (MB) removal process which is a rapid for all three samples, reaching equilibrium after 9 h, and optimal at neutral pH and maximum at the highest temperature, 40 °C. The MB adsorption capacity was highest for KM (191.07 mg/g), followed by the KCB and KBS samples. Isotherm modelling of the samples showed best fits for KBS, KCB and KM as Langmuir-Freundlich (LF), Langmuir and Toth models respectively. On the contrary, kinetic modelling using contact time study data for all samples exhibited best fits by the Diffusion-chemisorption (DC) model. Finally, the thermodynamic calculations of the mixed sample disclosed the adsorption process to be exothermic and spontaneous, with potential mechanisms being electrostatic attraction, ion exchange, π-π interactions, and hydrogen bonding. Multiple cycles of KM regeneration was also achieved with good adsorption capacities. Future work will explore other activation methods and examine the magnetic properties of KBS and KM for real water treatment.

Emerging environmental health risks associated with the land application of biosolids: a scoping review.

BACKGROUND: Over 40% of the six million dry metric tons of sewage sludge, often referred to as biosolids, produced annually in the United States is land applied. Biosolids serve as a sink for emerging pollutants which can be toxic and persist in the environment, yet their fate after land application and their impacts on human health have not been well studied. These gaps in our understanding are exacerbated by the absence of systematic monitoring programs and defined standards for human health protection. METHODS: The purpose of this paper is to call critical attention to the knowledge gaps that currently exist regarding emerging pollutants in biosolids and to underscore the need for evidence-based testing standards and regulatory frameworks for human health protection when biosolids are land applied. A scoping review methodology was used to identify research conducted within the last decade, current regulatory standards, and government publications regarding emerging pollutants in land applied biosolids. RESULTS: Current research indicates that persistent organic compounds, or emerging pollutants, found in pharmaceuticals and personal care products, microplastics, and per- and polyfluoroalkyl substances (PFAS) have the potential to contaminate ground and surface water, and the uptake of these substances from soil amended by the land application of biosolids can result in contamination of food sources. Advanced technologies to remove these contaminants from wastewater treatment plant influent, effluent, and biosolids destined for land application along with tools to detect and quantify emerging pollutants are critical for human health protection. CONCLUSIONS: To address these current risks, there needs to be a significant investment in ongoing research and infrastructure support for advancements in wastewater treatment; expanded manufacture and use of sustainable products; increased public communication of the risks associated with overuse of pharmaceuticals and plastics; and development and implementation of regulations that are protective of health and the environment.

Diversity of virulence and antibiotic resistance genes expressed in Class A biosolids and biosolids-amended soil as revealed by metatranscriptomic analysis.

Class A biosolids is a treated sewage sludge, commonly applied to agricultural fields, home lawns/gardens, golf courses, forests, and remediation sites around the world. This practice is of public and agricultural concern due to the possibility that biosolids contain antibiotic-resistant bacteria and fungal pathogens that could persist for extended periods in soil. This possibility was determined by metatranscriptomic analysis of virulence, antibiotic resistance, and plasmid conjugation genes, a Class A biosolids, organically managed soil, and biosolids-amended soil under realistic conditions. Biosolids harbored numerous transcriptionally active pathogens, antibiotic resistance genes, and conjugative genes that annotated mostly to Gram-positive pathogens of animal hosts. Biosolids amendment to soil significantly increased the expression of virulence genes by numerous pathogens and antibiotic-resistant genes that were strongly associated with biosolids. Biosolids amendment also significantly increased the expression of virulence genes by native soil fungal pathogens of plant hosts, which suggests higher risks of crop damage by soil fungal pathogens in biosolids-amended soil. Although results are likely to be different in other soils, biosolids, and microbial growth conditions, they provide a more holistic, accurate view of potential health risks associated with biosolids and biosolids-amended soils than has been achievable with more selective cultivation and PCR-based techniques.

Potential ecotoxicological effects of silver nanoparticles and silver sulphide on the endogeic earthworm Aporrectodea caliginosa (Savigny 1826).

Silver nanoparticles (AgNPs) are increasingly used in consumer products and subsequently arrive in wastewater systems, accumulating as silver sulphide (Ag2S) in the resulting biosolids, which are commonly spread onto agricultural fields as a fertiliser. Experiments were performed to investigate the effect of AgNPs, using the endogeic earthworm Aporrectodea caliginosa as a test organism. In an acute toxicity experiment, A. caliginosa were exposed to soil containing different concentrations of AgNPs (0, 1, 5, 10, 50, 100, 250, 500, 750, and 1000 mg kg-1 dry soil) and Ag2S (0, 10, 50, 100, 500, and 1000 mg kg-1 dry soil). Earthworm biomass and mortality were monitored. Earthworms exposed to 500, 750 and 1000 mg kg-1 fresh AgNPs had mortality rates of 20%, 60% and 70%, respectively. Changes in biomass were directly related to AgNP concentration. Exposure to Ag2S did not affect biomass or mortality. Further experiments used 0, 10, 50, 100 and 250 mg kg-1 AgNPs and 0, 50, 100, 500, and 1000 mg kg-1 Ag2S to evaluate sublethal effects on A. caliginosa. Avoidance behaviour in a linear gradient was evaluated after 14 days. Earthworms significantly preferred soil that was free of either AgNPs or Ag2S. The same concentrations were used to assess effects on cocoon production of A. caliginosa exposed to AgNPs and Ag2S. In the first 3 months of AgNP exposure, higher concentrations had a negative effect on cocoon production, but this effect diminished thereafter. Ag2S had no discernible effect on reproduction. Overall, introduction of AgNPs into the soil through the application of biosolids appears to be of low concern to the tested endogeic earthworm.

The Effect of Municipal Biosolids on the Growth, Physiology and Synthesis of Phenolic Compounds in Ocimum basilicum L.

The continuous development of drinking water networks is leading to the production of increasing amounts of waste water and sewage sludge. Secondary-treated sewage sludge is called biosolids and can be used as fertilizers in agriculture due to its rich nutrient content. The aim of this study was to evaluate the effects of biosolids mixed with an eroded soil on the morphology, physiology and synthesis of bioactive compounds in basil. The study was performed in pots under laboratory-controlled conditions. In total, four substrates were tested: S1 biosolids 100%, S2 biosolids 15% + eroded soil 85%, S3 eroded soil 100% and S4 control (commercial growing substrate). At the morphological level, a significant increase in plant height, number of branches, fresh biomass and dry biomass was found in the S2 variant. At the physiological level, photosynthesis and chlorophyll content did not vary significantly, but the quantum yield of PSII (ΦPSII) was significantly higher at S1 and S2. The oxidative status evaluated by determining the activity of SOD, POD and CAT enzymes was better in S2 and S3 compared to S3. Regarding the synthesis of bioactive compounds (rosmarinic acid, caffeic acid and gallic acid), it was stimulated in S1 and S2. In conclusion, biosolids application stimulated the stress response mechanisms in basil plants by increasing the quantum yield chlorophyll fluorescence and catalase activity, alleviating the negative effects of eroded soil.

Leaching of select per-/poly-fluoroalkyl substances, pharmaceuticals, and hormones through soils amended with composted biosolids.

The use of organic amendments to enhance soil health is increasingly being identified as a strategy to improve residential landscapes while also reducing the need for external inputs (e.g., fertilizers, irrigation). Composted biosolids are a re-purposed waste product that can be used in organic amendments to improve the overall sustainability of a municipality by enhancing residential soil carbon content while simultaneously reducing waste materials. However, the biosolids-based feedstock of these compost products has the potential to be a source of organic contaminants. We conducted a laboratory-based soil column experiment to evaluate the potential for different commercially available compost products to act as a source of emerging organic contaminants in residential landscapes. We compared two biosolids-based compost products, a manure-based compost product, and a control (no compost) treatment by irrigating soil columns for 30 days and collecting daily leachate samples to quantify leaching rates of six hormones, eight pharmaceuticals, and seven per- and polyfluoroalkyl substances (PFAS). Detection of hormones and pharmaceuticals was rare, suggesting that compost amendments are likely not a major source of these contaminants to groundwater resources. In contrast, we detected three of the seven PFAS compounds in leachate samples throughout the study. Perfluorohexanoic acid (PFHxA) was more likely to leach from biosolids-based compost treatments than other treatments (p < 0.05) and perfluorobutane sulfonate (PFBS) was only detected in biosolids-based treatments (although PFBS concentrations did not significantly differ among treatments). In contrast, perfluorooctanoic acid (PFOA) was commonly detected across all treatments (including controls), suggesting potential PFOA experimental contamination. Overall, these results demonstrate that commercially available composted biosolids amendments are likely not a major source of hormone and pharmaceutical contamination. The detection of PFHxA at significantly higher concentrations in biosolids treatments suggests that biosolids-based composts may act as sources of PFHxA to the environment. However, concentrations of multiple PFAS compounds found in leachate in this study were lower than concentrations found in known PFAS hotspots. Therefore, there is potential for environmental contamination from PFAS leaching from composted biosolids, but leachate concentrations are low which should be considered in risk-benefit analyses when considering whether or not to use composted biosolids as an organic amendment to enhance residential soil health.

Steps to circularity: Impact of resource recovery and urban agriculture in Seattle and Tacoma, Washington.

Capturing the value in urban residuals (food scraps and wastewater) is a critical component of urban sustainability and a circular nutrient economy. Food production in urban areas has also been recognized as an important component of urban health. Data from two cities (Seattle and Tacoma, WA) with active resource recovery and community garden programs were used to quantify nutrient recovery and food production potential. Yield data from growth trials conducted using soil amendments produced from locally generated organic residuals were used to model yields in existing urban agriculture programs. Our survey showed much lower than expected volume of food scraps from both residential and multifamily housing for both cities. Nutrient generation rates from food scraps were estimated as 0.55-0.67 kg N and 0.09-0.11 kg P capita-1 yr-1. Recovery rates for Seattle with an established food scrap collection program were 0.21 kg N and 0.006 kg P capita-1 yr-1. Nutrient recovery from wastewater biosolids was higher; 1-1.67 kg N and 0.23-0.76 kg P capita-1 yr-1. Data on effluent quantity and nutrient concentrations from these programs suggests that effluent has a high potential for nutrient recovery (4.03-5 kg N and 0.3-0.5 kg P capita yr-1). Yield was modeled for kale (brassica oleracea) considering the number of people that could be fed per hectare for one year using a 67 g portion by comparing yields from synthetic fertilizer and residuals-based amendments in both high and low quality urban soils. The Tacoma biosolids potting soil yielded enough for 310 and 736 people ha-1 yr-1 for the high and low quality soils, respectively. The modeled food/yard compost produced from the food scraps yielded sufficient kale for 148 to 353 people ha-1 yr-1. Relative yield from fertilizer for the low and high quality soils was 15 and 263 people ha-1yr-1, respectively. Considering yield, enough biosolids are produced to meet 6.7-29.2% of the vegetable needs of each city. These results suggest that significant nutrients can be recovered using existing infrastructure. With enhanced nutrient capture from wastewater effluent, sufficient nutrients could be recovered to meet the N and P needs for food crops for the residents of each city.

Pulp mill biosolids mitigate soil greenhouse gas emissions from applied urea and improve soil fertility in a hybrid poplar plantation.

Pulp mill biosolids (hereafter 'biosolids') could be used as an organic amendment to improve soil fertility and promote crop growth; however, it is unclear how the application of biosolids affects soil greenhouse gas emissions and the mechanisms underlying these effects. Here, we conducted a 2-year field experiment on a 6-year-old hybrid poplar plantation in northern Alberta, Canada, to compare the effects of biosolids, conventional mineral fertilizer (urea), and urea + biosolids on soil CO2, CH4 N2O emissions, as well as soil chemical and microbial properties. We found that the addition of biosolids increased soil CO2 and N2O emissions by 21 and 17%, respectively, while urea addition increased their emissions by 30 and 83%, respectively. However, the addition of urea did not affect soil CO2 emissions when biosolids were also applied. The addition of biosolids and biosolids + urea increased soil dissolved organic carbon (DOC) and microbial biomass C (MBC), while urea addition and biosolids + urea addition increased soil inorganic N, available P and denitrifying enzyme activity (DEA). Furthermore, the CO2 and N2O emissions were positively, while the CH4 emissions were negatively associated with soil DOC, inorganic N, available phosphorus, MBC, microbial biomass N, and DEA. In addition, soil CO2, CH4 and N2O emissions were also strongly associated with soil microbial community composition. We conclude that the application of the combination of biosolids and chemical N fertilizer (urea) could be a beneficial approach for both the disposal and use of pulp mill wastes, by reducing greenhouse gas emissions and improving soil fertility.

The dynamics of boron when amending agricultural soil of the Mediterranean basin with biosolids: trials in leaching columns.

There is mainly a lack of boron (B) in soils with low amounts of organic matter and in acidic and sandy soils. This is especially true in irrigated land or humid regions, where leaching can occur. The results from studying the amount of available B will reveal the status of B in the soil of a specific plot of land. The experimentation was performed as a controlled study using leaching columns. A container was placed at the end of the columns to collect the infiltrated water. Three treatments were performed by applying different amounts of biosolids (T40: 40,000 kg ha-1, T80: 80,000 kg ha-1, T120: 120,000 kg ha-1), as well as a blank test or control treatment (T0). We conclude that the mobility of B in soil was generally low despite the addition of organic matter and humidity to the soil. This is an indication that there is no clear risk of aquifers being contaminated with B or plants being impacted by toxicity due to this micronutrient.

Combining biosolid and mineral sources of phosphorus and potassium in organomineral fertilizers influences the dynamics and efficiency of nutrient release.

Using urban residues to produce organomineral fertilizers (OMF) is an environmentally friendly strategy that can enhance soil fertility by adding organic matter and mineral nutrients. Herein we investigated the availability of N, P, and K, under organomineral fertilization in sandy soils. An incubation study was conducted using OMF formulated with biosolids as organic matrix and N source, rock phosphate, and potassium sulfate as P and K sources, respectively. Two forms of isolated N, P, and K sources (granulated and non-granulated), five N:P:K granulation proportions (1-2-0, 1-4-0, 1-0-2, 1-2-2, 1-2-4), and a control (unfertilized) were mixed with soil and assessed over a 112 days incubation period. Soil samples were collected at 0, 7, 14, 28, 56, and 112 days to quantify available soil concentrations of ammonium (N-NH4+), nitrate + nitrite (N-NO2- + N-NO3-), P, and K. The results showed that OMF formulated with NPK had better nitrogen efficiency indexes (NEI) than other formulations and did not induce N immobilization throughout the experiment. Regarding P and K efficiency, OMFs containing phosphorus and potassium increased the indexes compared to the single fertilizer sources. When comparing non-granulated potassium sulfate with granulated, the latter showed a steadier release due to the granulation process. In comparison with rock phosphate at the end of the experiment, the OMFs 1-2-0 and 1-4-2 had higher P available by 116 and 41%, respectively. Based on these results, OMFs have the potential to alter the dynamics of nutrient availability serving as a strategy for nutrient management in agriculture.

Biosolids for safe land application: does wastewater treatment plant size matters when considering antibiotics, pollutants, microbiome, mobile genetic elements and associated resistance genes?

Soil fertilization with wastewater treatment plant (WWTP) biosolids is associated with the introduction of resistance genes (RGs), mobile genetic elements (MGEs) and potentially selective pollutants (antibiotics, heavy metals, disinfectants) into soil. Not much data are available on the parallel analysis of biosolid pollutant contents, RG/MGE abundances and microbial community composition. In the present study, DNA extracted from biosolids taken at 12 WWTPs (two large-scale, six middle-scale and four small-scale plants) was used to determine the abundance of RGs and MGEs via quantitative real-time PCR and the bacterial and archaeal community composition was assessed by 16S rRNA gene amplicon sequencing. Concentrations of heavy metals, antibiotics, the biocides triclosan, triclocarban and quaternary ammonium compounds (QACs) were measured. Strong and significant correlations were revealed between several target genes and concentrations of Cu, Zn, triclosan, several antibiotics and QACs. Interestingly, the size of the sewage treatment plant (inhabitant equivalents) was negatively correlated with antibiotic concentrations, RGs and MGEs abundances and had little influence on the load of metals and QACs or the microbial community composition. Biosolids from WWTPs with anaerobic treatment and hospitals in their catchment area were associated with a higher abundance of potential opportunistic pathogens and higher concentrations of QACs.

Responses of the Soil Bacterial Community, Resistome, and Mobilome to a Decade of Annual Exposure to Macrolide Antibiotics.

Biosolids that are applied to agricultural soil as an organic fertilizer are frequently contaminated with pharmaceutical residues that have persisted during wastewater treatment and partitioned into the organic phase. Macrolide antibiotics, which serve as a critically important human medicine, have been detected within biosolids. To determine the impacts of macrolide antibiotics on soil bacteria, every year for a decade, a series of replicated field plots received an application of a mixture of erythromycin, clarithromycin, and azithromycin at a realistic (0.1 mg kg soil-1) or an unrealistically high (10 mg kg soil-1) dose or were left untreated. The effects of repeated antibiotic exposure on the soil bacterial community, resistome, mobilome, and integron gene cassette content were evaluated by 16S rRNA and integron gene cassette amplicon sequencing, as well as whole-metagenome sequencing. At the unrealistically high dose, the overall diversity of the resistome and mobilome was altered, as 21 clinically important antibiotic resistance genes predicted to encode resistance to 10 different antibiotic drug classes were increased and 20 mobile genetic element variants (tnpA, intI1, tnpAN, and IS91) were increased. In contrast, at the realistic dose, no effect was observed on the overall diversity of the soil bacterial community, resistome, mobilome, or integron gene cassette-carrying genes. Overall, these results suggest that macrolide antibiotics entrained into soil at concentrations anticipated with biosolid applications would not result in major changes to these endpoints. IMPORTANCE Biosolids, produced from the treatment of sewage sludge, are rich in plant nutrients and are a valuable alternative to inorganic fertilizer when applied to agricultural soil. However, the use of biosolids in agriculture, which are frequently contaminated with pharmaceuticals, such as macrolide antibiotics, may pose a risk to human health by selecting for antibiotic resistance genes that could be transferred to plant-based food destined for human consumption. The consequences of long-term, repeated macrolide antibiotic exposure on the diversity of the soil bacterial community, resistome, and mobilome were evaluated. At unrealistically high concentrations, macrolide antibiotics alter the overall diversity of the resistome and mobilome, enriching for antibiotic resistance genes and mobile genetic elements of concern to human health. However, at realistic antibiotic concentrations, no effect on these endpoints was observed, suggesting that current biosolids land management practices are unlikely to pose a risk to human health due to macrolide antibiotic contamination alone.

Environmental and Economic Impacts of Managing Nutrients in Digestate Derived from Sewage Sludge and High-Strength Organic Waste.

Increasingly stringent limits on nutrient discharges are motivating water resource recovery facilities (WRRFs) to consider the implementation of sidestream nutrient removal or recovery technologies. To further increase biogas production and reduce landfilled waste, WRRFs with excess anaerobic digestion capacity can accept other high-strength organic waste (HSOW) streams. The goal of this study was to characterize and evaluate the life-cycle global warming potential (GWP), eutrophication potential, and economic costs and benefits of sidestream nutrient management and biosolid management strategies following digestion of sewage sludge augmented by HSOW. Five sidestream nutrient management strategies were analyzed using environmental life-cycle assessment (LCA) and life-cycle cost analysis (LCCA) for codigestion of municipal sewage sludge with and without HSOW. As expected, thermal stripping and ammonia stripping were characterized by a much lower eutrophication potential than no sidestream treatment; significantly higher fertilizer prices would be needed for this revenue stream to cover the capital and chemical costs. Composting all biosolids dramatically reduced the GWP relative to the baseline biosolid option but had slightly higher eutrophication potential. These complex environmental and economic tradeoffs require utilities to consider their social, environmental, and economic values in addition to present or upcoming nutrient discharge limits prior to making decisions in sidestream and biosolids management.

Identification and Quantification of Micro-Bioplastics in Environmental Samples by Pyrolysis-Gas Chromatography-Mass Spectrometry.

Bioplastics are materials that are biobased and/or biodegradable, but not necessarily both. Concerns about environmental plastic pollution are constantly growing with increasing demand for substituting fossil-based plastics with those made using renewable resource feedstocks. For many conventional bioplastics to completely decompose/degrade, they require specific environmental conditions that are rarely met in natural ecosystems, leading to rapid formation of micro-bioplastics. As global bioplastic production and consumption/use continue to increase, there is growing concern regarding the potential for environmental pollution from micro-bioplastics. However, the actual extent of their environmental occurrence and potential impacts remains unclear, and there is insufficient mass concentration-based quantitative data due to the lack of quantitative analytical methods. This study developed and validated an analytical method coupling pressurized liquid extraction and pyrolysis-gas chromatography-mass spectrometry combined with thermochemolysis to simultaneously identify and quantify five targeted micro-bioplastics (i.e., polylactic acid (PLA), polyhydroxyalkanoate, polybutylene succinate, polycaprolactone, and polybutylene adipate terephthalate (PBAT)) in environmental samples on a polymer-specific mass-based concentration. The recovery of spiked micro-bioplastics in environmental samples (biosolids) ranged from 74 to 116%. The limits of quantification for the target micro-bioplastics were between 0.02 and 0.05 mg/g. PLA and PBAT were commonly detected in wastewater, biosolids, and sediment samples at concentrations between 0.07 and 0.18 mg/g. The presented analytical method enables the accurate identification, quantification, and monitoring of micro-bioplastics in environmental samples. This study quantified five micro-bioplastic types in complex environmental samples for the first time, filling in gaps in our knowledge about bioplastic pollution and providing a useful methodology and important reference data for future research.

Target and Nontarget Screening of PFAS in Biosolids, Composts, and Other Organic Waste Products for Land Application in France.

Zwitterionic, cationic, and anionic per- and polyfluoroalkyl substances (PFAS) are increasingly reported in terrestrial and aquatic environments, but their inputs to agricultural lands are not fully understood. Here, we characterized PFAS in 47 organic waste products (OWP) applied in agricultural fields of France, including historical and recent materials. Overall, 160 PFAS from 42 classes were detected from target screening and homologue-based nontarget screening. Target PFAS were low in agriculture-derived wastes such as pig slurry, poultry manure, or dairy cattle manure (median ∑46PFAS: 0.66 µg/kg dry matter). Higher PFAS levels were reported in urban and industrial wastes, paper mill sludge, sewage sludge, or residual household waste composts (median ∑46PFAS: 220 µg/kg). Historical municipal biosolids and composts (1976-1998) were dominated by perfluorooctanesulfonate (PFOS), N-ethyl perfluorooctanesulfonamido acetic acid (EtFOSAA), and cationic and zwitterionic electrochemical fluorination precursors to PFOS. Contemporaneous urban OWP (2009-2017) were rather dominated by zwitterionic fluorotelomers, which represented on average 55% of ∑160PFAS (max: 97%). The fluorotelomer sulfonamidopropyl betaines (X:2 FTSA-PrB, median: 110 µg/kg, max: 1300 µg/kg) were the emerging class with the highest occurrence and prevalence in contemporary urban OWP. They were also detected as early as 1985. The study informs for the first time that urban sludges and composts can be a significant repository of zwitterionic and cationic PFAS.