Heavy metal stress and mitogen activated kinase transcription factors in plants: Exploring heavy metal-ROS influences on plant signalling pathways.

Due to their stationary nature, plants are exposed to a diverse range of biotic and abiotic stresses, of which heavy metal (HM) stress poses one of the most detrimental abiotic stresses, targeting diverse plant processes. HMs instigate the overproduction of reactive oxygen species (ROS), and to mitigate the adverse effects of ROS, plants induce multiple defence mechanisms. Besides the negative implications of overproduction of ROS, these molecules play a multitude of signalling roles in plants, acting as a central player in the complex signalling network of cells. One of the ROS-associated signalling mechanisms is the mitogen-activated protein kinase (MAPK) cascade, a signalling pathway which transduces extracellular stimuli into intracellular responses. Plant MAPKs have been implicated in signalling involved in stress response, phytohormone regulation, and cell cycle cues. However, the influence of various HMs on MAPK activation has not been well documented. In this review, we address and summarise several aspects related to various HM-induced ROS signalling. Additionally, we touch on how these signals activate the MAPK cascade and the downstream transcription factors that influence plant responses to HMs. Moreover, we propose a workflow that could characterise genes associated with MAPKs and their roles during plant HM stress responses.

The role of strigolactones in resistance to environmental stress in plants.

Abiotic stress impairs plant growth and development, thereby causing low yield and inferior quality of crops. Increasing studies reported that strigolactones (SL) are plant hormones that enhance plant stress resistance by regulating plant physiological processes and gene expressions. In this review, we introduce the response and regulatory role of SL in salt, drought, light, heat, cold and cadmium stresses in plants. This review also discusses how SL alleviate the damage of abiotic stress in plants, furthermore, introducing the mechanisms of SL enhancing plant stress resistance at the genetic level. Under abiotic stress, the exogenous SL analog GR24 can induce the biosynthesis of SL in plants, and endogenous SL can alleviate the damage caused by abiotic stress. SL enhanced the stress resistance of plants by protecting photosynthesis, enhancing the antioxidant capacity of plants and promoting the symbiosis between plants and arbuscular mycorrhiza (AM). SL interact with abscisic acid (ABA), salicylic acid (SA), auxin, cytokinin (CK), jasmonic acid (JA), hydrogen peroxide (H2O2) and other signal molecules to jointly regulate plant stress resistance. Lastly, both the importance of SL and their challenges for future work are outlined in order to further elucidate the specific mechanisms underlying the roles of SL in plant responses to abiotic stress.

Nitrogen supply neutralizes the nanoplastic-plant interaction in a coastal wetland.

The presence of nanoplastics posed a potential threat to coastal saline-alkaline wetlands where nitrogen (N) fertilizer is being implemented as an important ecological restoration measure. Notwithstanding, the effects of N inputs on plant community in polypropylene-nanoplastics (PP-NPs) coexistence environments are largely unknown. To address this, we investigated the effects of PP-NPs addition alone or combined N supply on community aboveground biomass, morphological traits, diversity, composition, niche differentiation, interspecific interactions, and assembly. Our results showed that the PP-NPs addition alone reduced community aboveground biomass and morphological traits. However, the addition of high concentration (0.5%) PP-NPs alone favored community α-diversity and reduced community stability, which could be weakened through combined N supply. Overall, the effect of PP-NPs addition alone on plant community composition was greater than that of combined N supply. We also demonstrated PP-NPs addition alone and combined N supply reduced the niche breadth of the plant community and affected the niche overlap of dominant species. In the assembly of plant communities, stochastic processes played a dominant role. We conclude that N fertilization can amend the terrestrial nanoplastics pollution, thus mitigating the effects of PP-NPs on the plant community.

Interactions shape aquatic microbiome responses to Cu and Au nanoparticle treatments in wetland manipulation experiments.

In natural systems, organisms are embedded in complex networks where their physiology and community composition is shaped by both biotic and abiotic factors. Therefore, to assess the ecosystem-level effects of contaminants, we must pair complex, multi-trophic field studies with more targeted hypothesis-driven approaches to explore specific actors and mechanisms. Here, we examine aquatic microbiome responses to long-term additions of commercially-available metallic nanoparticles [copper-based (CuNPs) or gold (AuNPs)] and/or nutrients in complex, wetland mesocosms over 9 months, allowing for a full growth cycle of the aquatic plants. We found that both CuNPs and AuNPs (but not nutrient) treatments showed shifts in microbial communities and populations largely at the end of the experiment, as the aquatic plant community senesced. we examine aquatic microbiomes under chronic dosing of NPs and nutrients Simplified microbe-only or microbe + plant incubations revealed that direct effects of AuNPs on aquatic microbiomes can be buffered by plants (regardless of seasonal As mesocosms were dosed weekly, the absence of water column accumulation indicates the partitioning of both metals into other environmental compartments, mainly the floc and aquatic plants photosynthetically-derived organic matter. Overall, this study identifies the potential for NP environmental impacts to be either suppressed by or propagated across trophic levels via the presence of primary producers, highlighting the importance of organismal interactions in mediating emerging contaminants' ecosystem-wide impacts.

Unveiling the impact of microplastics and nanoplastics on vascular plants: A cellular metabolomic and transcriptomic review.

With increasing plastic manufacture and consumption, microplastics/nanoplastics (MP/NP) pollution has become one of the world's pressing global environmental issues, which poses significant threats to ecosystems and human health. In recent years, sharp increasing researches have confirmed that MP/NP had direct or indirect effects on vegetative growth and sexual process of vascular plant. But the potential mechanisms remain ambiguous. MP/NP particles can be adsorbed and/or absorbed by plant roots or leaves and thus cause diverse effects on plant. This holistic review aims to discuss the direct effects of MP/NP on vascular plant, with special emphasis on the changes of metabolic and molecular levels. MP/NP can alter substance and energy metabolism, as well as shifts in gene expression patterns. Key aspects affected by MP/NP stress include carbon and nitrogen metabolism, amino acids biosynthesis and plant hormone signal transduction, expression of stress related genes, carbon and nitrogen metabolism related genes, as well as those involved in pathogen defense. Additionally, the review provides updated insights into the growth and physiological responses of plants exposed to MP/NP, encompassing phenomena such as seed/spore germination, photosynthesis, oxidative stress, cytotoxicity, and genotoxicity. By examining the direct impact of MP/NP from both physiological and molecular perspectives, this review sets the stage for future investigations into the complex interactions between plants and plastic pollutants.

Spatial, temporal, and biological factors influencing plant responses to deicing salt in roadside bioinfiltration basins.

Plants are arguably the most visible components of stormwater bioretention basins and play key roles in stabilizing soils and removing water through transpiration. In regions with cold winters, bioretention basins along roadways can receive considerable quantities of deicing salt, much of which migrates out of the systems prior to the onset of plant growth but the rest remains in the soil. The resulting effects on plants presumably vary with time (due to annual weather patterns), space (because stormwater exposure is location-dependent), and biology (because plant taxa differ in their salt tolerance). The goal of this study was to investigate the magnitude of deicing salt's effects on bioretention plants and how it varies with spatial, temporal, and biological factors. The study took place in a set of five bioretention basins in Philadelphia, USA that receive runoff from a major highway. Over a five-year period, the electrical conductivity (EC) of influent stormwater frequently exceeded 1 mS cm-1 in winter, and occasionally surpassed that of seawater (∼50 mS cm-1). In both of the years when soil EC was measured as well, it remained elevated through all spring months, especially near basin inlets and centers. Mortality of nine plant taxa ranged widely after three years (0-90%), with rankings largely corresponding to salt tolerances. Moreover, leaf areas and/or crown volumes were strongly reduced in proportion to stormwater exposure in seven of these taxa. In the three taxa evaluated for tissue concentrations of 14 potentially toxic elements (Hemerocallis 'Happy Returns', Iris 'Caesar's Brother', and Cornus sericea 'Cardinal'), only sodium consistently exceeded the toxicity limit for salt intolerant plants (500 mg kg-1). However, exceedance of the sodium toxicity limit was associated with plants' topographic positions, with median concentrations greatest in the bottom of basins and least on basin rims. This study demonstrates that deicing salts can have detrimental effects on plants in bioretention basins, with the strongest effects likely to occur in years with the greatest snowfall (and therefore deicing salt use), in portions of basins with greatest stormwater exposure (typically around inlets and centers), and in plants with minimal salinity tolerance. Our results therefore underscore the value of installing salt-tolerant taxa in basins likely to experience any frequency of deicing salt exposure.

Metallic nanoparticles and photosynthesis organisms: Comprehensive review from the ecological perspective.

This review presents a comprehensive analysis of the ecological implications of metallic nanoparticles (MNPs) on photosynthetic organisms, particularly plants and algae. We delve into the toxicological impacts of various MNPs, including gold, silver, copper-based, zinc oxide, and titanium dioxide nanoparticles, elucidating their effects on the growth and health of these organisms. The article also summarizes the toxicity mechanisms of these nanoparticles in plants and algae from previous research, providing insight into the cellular and molecular interactions that underpin these effects. Furthermore, it discusses the reciprocal interactions between different types of MNPs, their combined effects with other metal contaminants, and compares the toxicity between MNPs with their counterpart. This review highlights the urgent need for a deeper understanding of the environmental impact, considering their escalating use and the potential risks they pose to ecological systems, especially in the context of photosynthetic organisms that are vital to ecosystem health and stability.

Recent advances on environmental behavior of Cu-based nanomaterials in soil-plant system: A review.

In recent years, copper-based nanomaterials (Cu-based NMs) have shown great potential in promoting agriculture development due to their special physicochemical characteristics. With the mass production and overuse of Cu-based NMs, there are potential effects on the soil-plant environment. Soil organisms, especially soil microorganisms, play a significant part in terrestrial or soil ecosystems; plants, as indirect organisms with soil-related Cu-based NMs, may affect human health through plant agricultural products. Understanding the accumulation and transformation of Cu-based NMs in soil-plant systems, as well as their ecotoxicological effects and potential mechanisms, is a prerequisite for the scientific assessment of environmental risks and safe application. Therefore, based on the current literature, this review: (i) introduces the accumulation and transformation behaviors of Cu-based NMs in soil and plant systems; (ii) focuses on the ecotoxicological effects of Cu-based NMs on a variety of organisms (microorganisms, invertebrates, and plants); (iii) reveals their corresponding toxicity mechanisms. It appears from studies hitherto made that both Cu-based NMs and released Cu2+ may be the main reasons for toxicity. When Cu-based NMs enter the soil-plant environment, their intrinsic physicochemical properties, along with various environmental factors, could also affect their transport, transformation, and biotoxicity. Therefore, we should push for intensifying the multi-approach research that focuses on the behaviors of Cu-based NMs in terrestrial exposure environments, and mitigates their toxicity to ensure the promotion of Cu-based NMs.

Hydrogen peroxide-induced stress acclimation in plants.

Among all reactive oxygen species (ROS), hydrogen peroxide (H2O2) takes a central role in regulating plant development and responses to the environment. The diverse role of H2O2 is achieved through its compartmentalized synthesis, temporal control exerted by the antioxidant machinery, and ability to oxidize specific residues of target proteins. Here, we examine the role of H2O2 in stress acclimation beyond the well-studied transcriptional reprogramming, modulation of plant hormonal networks and long-distance signalling waves by highlighting its global impact on the transcriptional regulation and translational machinery.

Narrow-Band Red-Emitting Phosphors with High Color Purity, Trifling Thermal and Concentration Quenching for Hybrid White LEDs and Li3Y3BaSr(MoO4)8:Sm3+, Eu3+-Based Deep-Red LEDs for Plant Growth Applications.

Trivalent europium-based monochromatic red light-emitting phosphors are an essential component to realize high-performance smart lighting devices; however, the concentration and thermal quenching restrict their usage. Here, we report a series of efficient Eu3+-substituted Li3Y3BaSr(MoO4)8 red-emitting phosphors based on a stratified scheelite structure with negligible concentration and thermal quenching. All of the host and phosphor compositions crystallize in monoclinic crystal structure (space group C2/c). All of the phosphor compositions produce narrow-band red emission (FWHM ∼6 nm), which is highly apparent to the human eyes, and lead to exceptional chromatic saturation of the red spectral window. Concurrently, detailed investigations were carried out to comprehend the concentration and thermal quenching mechanism. Absolute quantum yields as high as 88.5% were obtained for Li3Y0.3Eu2.7BaSr(MoO4)8 phosphor with virtuous thermal stability (at 400 K, retaining 87% of its emission intensity). The light-emitting diodes were constructed by coupling Li3BaSrY0.3Eu2.7(MoO4)8 red phosphor with a near-UV LED chip (395 nm) operated at 20 mA forward bias, and the hybrid white LED (an organic yellow dye + red Li3Y3BaSr(MoO4)8:Eu3+ phosphor integrated with an NUV LED chip) showed a low CCT (6645 K), high CRI (83) values, and CIE values of x = 0.303; y = 0.368, which indicated that the synthesized phosphors can be a suitable red component for white LEDs. In addition, we have systematically investigated the Sm3+ and Sm3+, Eu3+ activation in Li3Y3BaSr(MoO4)8 to display the latent use of the system in plant growth applications and establish that the phosphor exhibits orange red emission with an intense deep-red emission (645 nm (4G5/2 → 6H9/2)). The phytochrome (Pr) absorption spectrum well matched the fabricated deep-red LED (by integrating a NUV LED + Li3Y3BaSr(MoO4)8:Sm3+ and Eu3+ phosphor) spectral lines.

Role of Silica Nanoparticles in Abiotic and Biotic Stress Tolerance in Plants: A Review.

The demand for agricultural crops continues to escalate with the rapid growth of the population. However, extreme climates, pests and diseases, and environmental pollution pose a huge threat to agricultural food production. Silica nanoparticles (SNPs) are beneficial for plant growth and production and can be used as nanopesticides, nanoherbicides, and nanofertilizers in agriculture. This article provides a review of the absorption and transportation of SNPs in plants, as well as their role and mechanisms in promoting plant growth and enhancing plant resistance against biotic and abiotic stresses. In general, SNPs induce plant resistance against stress factors by strengthening the physical barrier, improving plant photosynthesis, activating defensive enzyme activity, increasing anti-stress compounds, and activating the expression of defense-related genes. The effect of SNPs on plants stress is related to the physical and chemical properties (e.g., particle size and surface charge) of SNPs, soil, and stress type. Future research needs to focus on the "SNPs-plant-soil-microorganism" system by using omics and the in-depth study of the molecular mechanisms of SNPs-mediated plant resistance.

Metalloprotein-Specific or Critical Amino Acid Residues: Perspectives on Plant-Precise Detoxification and Recognition Mechanisms under Cadmium Stress.

Cadmium (Cd) pollution in cultivated land is caused by irresistible geological factors and human activities; intense diffusion and migration have seriously affected the safety of food crops. Plants have evolved mechanisms to control excessive influx of Cd in the environment, such as directional transport, chelation and detoxification. This is done by some specific metalloproteins, whose key amino acid motifs have been investigated by scientists one by one. The application of powerful cell biology, crystal structure science, and molecular probe targeted labeling technology has identified a series of protein families involved in the influx, transport and detoxification of the heavy metal Cd. This review summarizes them as influx proteins (NRAMP, ZIP), chelating proteins (MT, PDF), vacuolar proteins (CAX, ABCC, MTP), long-distance transport proteins (OPT, HMA) and efflux proteins (PCR, ABCG). We selected representative proteins from each family, and compared their amino acid sequence, motif structure, subcellular location, tissue specific distribution and other characteristics of differences and common points, so as to summarize the key residues of the Cd binding target. Then, we explain its special mechanism of action from the molecular structure. In conclusion, this review is expected to provide a reference for the exploration of key amino acid targets of Cd, and lay a foundation for the intelligent design and breeding of crops with high/low Cd accumulation.

What Worth the Garlic Peel.

Plants have two types of reproduction: sexual, resulting in embryo production, and asexual, resulting in vegetative bodies commonly derived from stems and roots (e.g., bulb, tuber). Dead organs enclosing embryos (DOEEs, such as seed coat and pericarp) are emerging as central components of the dispersal unit acting to nurture the embryo and ensure its survival in the habitat. Here we wanted to investigate the properties of dead organs enclosing plant asexual reproductive bodies, focusing on the garlic (Allium sativum) bulb. We investigated the biochemical and biological properties of the outer peel enclosing the bulb and the inner peel enclosing the clove using various methodologies, including bioassays, proteomics, and metabolomics. The garlic peels differentially affected germination and post-germination growth, with the outer peel demonstrating a strong negative effect on seed germination of Sinapis alba and on post-germination growth of Brassica juncea. Proteome analysis showed that dead garlic peels possess 67 proteins, including chitinases and proteases, which retained their enzymatic activity. Among primary metabolites identified in garlic peels, the outer peel accumulated multiple sugars, including rhamnose, mannitol, sorbitol, and trehalose, as well as the modified amino acid 5-hydroxylysine, known as a major component of collagen, at a higher level compared to the clove and the inner peel. Growth of Escherichia coli and Staphylococcus aureus was promoted by garlic peel extracts but inhibited by clove extract. All extracts strongly inhibited spore germination of Fusarium oxysporum f.sp. melonis. Thus, the garlic peels not only provide physical protection to vegetative offspring but also appear to function as a refined arsenal of proteins and metabolites for enhancing growth and development, combating potential pathogens, and conferring tolerance to abiotic stresses.

Design, Synthesis and Antifungal/Nematicidal Activity of Novel 1,2,4-Oxadiazole Derivatives Containing Amide Fragments.

Plant diseases that are caused by fungi and nematodes have become increasingly serious in recent years. However, there are few pesticide chemicals that can be used for the joint control of fungi and nematodes on the market. To solve this problem, a series of novel 1,2,4-oxadiazole derivatives containing amide fragments were designed and synthesized. Additionally, the bioassays revealed that the compound F15 demonstrated excellent antifungal activity against Sclerotinia sclerotiorum (S. sclerotiorum) in vitro, and the EC50 value of that was 2.9 µg/mL, which is comparable with commonly used fungicides thifluzamide and fluopyram. Meanwhile, F15 demonstrated excellent curative and protective activity against S. sclerotiorum-infected cole in vivo. The scanning electron microscopy results showed that the hyphae of S. sclerotiorum treated with F15 became abnormally collapsed and shriveled, thereby inhibiting the growth of the hyphae. Furthermore, F15 exhibited favorable inhibition against the succinate dehydrogenase (SDH) of the S. sclerotiorum (IC50 = 12.5 µg/mL), and the combination mode and binding ability between compound F15 and SDH were confirmed by molecular docking. In addition, compound F11 showed excellent nematicidal activity against Meloidogyne incognita at 200 µg/mL, the corrected mortality rate was 93.2%, which is higher than that of tioxazafen.

Geraniol and Carvacrol in Essential Oil Bearing Thymus pulegioides: Distribution in Natural Habitats and Phytotoxic Effect.

Phenolic and non-phenolic chemotypes of Thymus pulegioides L. are common in Europe. Essential oils of these chemotypes, as various compositions of allelochemicals, can have different phytotoxic effects on neighboring plants in natural habitats. The aim of this study was to establish the distribution of carvacrol and geraniol in T. pulegioides, growing wild in Lithuania, and compare phytotoxity of essential oils of carvacrol and geraniol chemotypes on selected plant species. In investigating 131 T. pulegioides habitats, essential oils were isolated by hydrodistillation and analyzed by GC-FID and GC-MS. Phytotoxity of essential oils extracted from carvacrol and geraniol chemotypes transmitted through water and air to selected plants was determined under laboratory conditions. Pharmacologically valuable Hypericum perforatum L. and the important forage grass Phleum pratense L. were respectively selected for experimentation from among 35 medicinal plants and 10 feed Poaceae species, growing in T. pulegioides habitats. Field results showed that carvacrol is common throughout Lithuania's territory, whereas the geraniol is predominantly located under the continental climatic conditions of the eastern region of the country. In the laboratory experiment, it was established that there was stronger inhibition of P. pratense seed germination by the essential oil of the geraniol chemotype than the carvacrol chemotype. None of the H. perforatum seeds germinated after exposure to the essential oil of the geraniol chemotype. In general, this study builds on previous studies by providing further evidence that different T. pulegioides chemotypes have contrasting phytotoxic effects on neighboring plants within their natural habitats.

Phytotoxicity of particulate matter from controlled burning of different plastic waste types.

According to careful estimations, open burning of plastic waste affects app. 2 billion people worldwide. While human health risks have become more and more obvious, much less information is available on the phytotoxicity of these emissions. In our study phytotoxicity of particulate matter samples generated during controlled combustion of different plastic waste types such as polyvinyl chloride (PVC), polyurethane (PUR), polypropylene (PP), polystyrene (PS) and polyethylene (PE) was evaluated based on peroxidase levels. While different samples showed different concentration-effect relationship patterns, higher concentration(s) caused decreased peroxidase activities in each sample indicating serious damage.

Advances in Chemical Priming to Enhance Abiotic Stress Tolerance in Plants.

Abiotic stress is considered a major factor limiting crop yield and quality. The development of effective strategies that mitigate abiotic stress is essential for sustainable agriculture and food security, especially with continuing global population growth. Recent studies have demonstrated that exogenous treatment of plants with chemical compounds can enhance abiotic stress tolerance by inducing molecular and physiological defense mechanisms, a process known as chemical priming. Chemical priming is believed to represent a promising strategy for mitigating abiotic stress in crop plants. Plants biosynthesize various compounds, such as phytohormones and other metabolites, to adapt to adverse environments. Research on artificially synthesized compounds has also resulted in the identification of novel compounds that improve abiotic stress tolerance. In this review, we summarize current knowledge of both naturally synthesized and artificial priming agents that have been shown to increase the abiotic stress tolerance of plants.

3'-(Phenyl alkynyl) analogs of abscisic acid: synthesis and biological activity of potent ABA antagonists.

We report here the synthesis and biological testing of 3'-(phenyl alkynyl) abscisic ABA analogs, a new class of potent ABA antagonists. These ABA analogs incorporate a rigid framework of eight carbon atoms attached at the 3'-carbon atom of ABA that prevents folding of the ABA analog-bound receptor required for ABA signalling. The two-step synthesis is based upon the optimized conversion of natural (S)-ABA to 3'-iodo ABA which can be coupled to phenyl acetylenes using Sonogashira conditions, or to styryl compounds through Suzuki chemistry. The parent 3'-(phenyl alkynyl) ABA analog 7 was obtained in 29% yield, 74% yield based on recovered starting material. In a lentil seed germination assay, compound 7 was found to have more potent activity than other known 3'-substituted ABA antagonists to date. In a structure activity study parasubstituted phenyl alkynyl analogs had comparable activity to the analog 7 while the 3'-styryl ABA 18 was only slightly less active. Analog 7 overcame ABA inhibition of germination and seedling growth in a wide range of mono and dicot plant species, including canola, lentil, soybean, rice, wheat, barley, cannabis and canary seed. 3'-(Phenyl alkynyl) ABA analogs have numerous potential practical agricultural applications including promoting ripening of crops, dormancy breaking of seeds and woody perennials, as well as promoting seed germination, and growth under stress conditions as demonstrated in this report.

Tissue culture-induced DNA methylation in crop plants: a review.

Plant tissue culture techniques have been extensively employed in commercial micropropagation to provide year-round production. Tissue culture regenerants are not always genotypically and phenotypically similar. Due to the changes in the tissue culture microenvironment, plant cells are exposed to additional stress which induces genetic and epigenetic instabilities in the regenerants. These changes lead to tissue culture-induced variations (TCIV) which are also known as somaclonal variations to categorically specify the inducing environment. TCIV includes molecular and phenotypic changes persuaded in the in vitro culture due to continuous sub-culturing and tissue culture-derived stress. Epigenetic variations such as altered DNA methylation pattern are induced due to the above-mentioned factors. Reportedly, alteration in DNA methylation pattern is much more frequent in the plant genome during the tissue culture process. DNA methylation plays an important role in gene expression and regulation of plant development. Variants originated in tissue culture process due to heritable methylation changes, can contribute to intra-species phenotypic variation. Several molecular techniques are available to detect DNA methylation at different stages of in vitro culture. Here, we review the aspects of TCIV with respect to DNA methylation and its effect on crop improvement programs. It is anticipated that a precise and comprehensive knowledge of molecular basis of in vitro-derived DNA methylation will help to design strategies to overcome the bottlenecks of micropropagation system and maintain the clonal fidelity of the regenerants.

Copper: uptake, toxicity and tolerance in plants and management of Cu-contaminated soil.

Copper (Cu) is an essential mineral nutrient for the proper growth and development of plants; it is involved in myriad morphological, physiological, and biochemical processes. Copper acts as a cofactor in various enzymes and performs essential roles in photosynthesis, respiration and the electron transport chain, and is a structural component of defense genes. Excess Cu, however, imparts negative effects on plant growth and productivity. Many studies have summarized the adverse effects of excess Cu on germination, growth, photosynthesis, and antioxidant response in agricultural crops. Its inhibitory influence on mineral nutrition, chlorophyll biosynthesis, and antioxidant enzyme activity has been verified. The current review focuses on the availability and uptake of Cu by plants. The toxic effects of excess Cu on seed germination, plant growth and development, photosynthesis, and antioxidant response in plants are discussed. Plant tolerance mechanisms against Cu stress, and management of Cu-contaminated soils are presented.