Antibodies to photoproducts of deoxyribonucleic acids irradiated with ultraviolet light.

A rabbit immunized with complexes of methylated bovine serum albumin and ultraviolet-irradiated DNA from calf thymus produced antibodies directed toward the photoproducts in the DNA. Serologic activity appeared after irradiation of DNA at 270 mmicro and decreased upon irradiation at 235 mmicro. The antigenic determinants of the ultraviolet-treated DNA appear to be photoproducts associated primarily with thymine, as measured by direct dependence of serologic activity on the adenine-thymine content of the DNA, and by inhibition of the Serlolgic reaction by the irradiated di-,tri-,and tetra-(thymidine-5'-phosphate nucleotides.

[Determination of radioresistance of microbes of different species irradiated separately and jointly]. / Opredelenie radiorezistentnosti mikrobov raznykh vidov pri razdel'nom i sovmestnom obluchenii.

Radioresistance of lactose positive and lactose negative Esch. coli, lactose positive Esch. coli and proteus, yellow sarcina and white staphylococcus, white and pink yeasts, white and yellow staphylococcus was studied after irradiation and seeding on hard media using separate and combined methods. In most cases radioresistance of the microbes did not depend of exposure conditions. However, lactose positive Esch. coli irradiated with proteus increased proteus radioresistance to its own standard. Yellow staphylococcus cannot form yellow pigment after irradiation with a dose of 1000 Gy.

[Quantitative and qualitative characteristics of representatives of the enterobacteriaceae family in postradiation intestinal dysbacteriosis]. / Kolichestvennaia i kachestvennaia kharakteristika predstavitelei semeistva énterobakterii pri postluchevom disbakterioze kishechnika.

The irradiation of CBA mice with gamma quanta in a dose of 700 C/kg resulted in the development of postradiation intestinal dysbacteriosis in the animals. The dysbacteriosis was characterized by a considerable increase in the number of Escherichia and Proteus mirabilis in the large intestine and by the insemination of the small intestine with these microbial associations. Pr. vulgaris, Pr. morganii, Ent. aerogenes, Ent. cloacae, Citrobacter appeared in great numbers in the intestinal tract of the irradiated mice, while none of these organisms were found in the intact mice.