Characteristics of a novel temperate bacteriophage against Staphylococcus arlettae (vB_SarS_BM31) / Características de un nuevo bacteriófago templado contra Staphylococcus arlettae (vB_SarS_BM31)
Int. microbiol
; 26(2): 327-341, May. 2023. ilus, graf
Article
en En
| IBECS
| ID: ibc-220225
Biblioteca responsable:
ES1.1
Ubicación: ES15.1 - BNCS
ABSTRACT
Background:
Staphylococcus arlettae is a rarely reported coagulase-negative staphylococcus (CoNS) isolated from infected humans and livestock. Observing phage-bacteria interaction could improve the understanding of bacterial pathogenetic mechanisms, providing foundational evidence for phage therapy or phage detection. Herein, we aimed to characterise and annotate a novel bacteriophage, vB_SarS_BM31 (BM31), specific to S. arlettae. This bacteriophage was isolated from a milk sample associated with bovine mastitis and collected in the Sichuan Province, China.Results:
The BM31 genome comprised a linear double-stranded DNA of 42,271 base pair in length with a G + C content of 34.59%. A total of 65 open reading frames (ORFs) were assembled from phage DNA, of which 29 were functionally annotated. These functional genes were divided into four modules the structural, DNA packing and replication, lysis, and lysogeny modules. Holin (ORF25), lysin (ORF26), and integrase (ORF28) were located closely in the entire BM31 genome and were important for lyse or lysogeny cycle of BM31. The phage was identified as a temperate phage according to whole genome analysis and life cycle assay, with basic biological characteristics such as small burst size, short latency period, and narrow host range, consistent with the characteristics of the family Siphoviridae, subcluster B14 of the Staphylococcus bacteriophage.Conclusions:
The present isolation and characterisation of BM31 contributes to the Staphylococcus bacteriophage database and provides a theoretical foundation for its potential applications. To the best of our knowledge, BM31 is the only shared and completely reported phage against S. arlettae in the entire public database.(AU)Palabras clave
Texto completo:
1
Colección:
06-national
/
ES
Banco de datos:
IBECS
Asunto principal:
Integrasas
/
34726
/
Lisogenia
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Mastitis
Límite:
Humans
Idioma:
En
Revista:
Int. microbiol
Año:
2023
Tipo del documento:
Article