Induction of metaphase arrest in cleaving Xenopus embryos by the protein kinase p90Rsk.
Science
; 286(5443): 1365-7, 1999 Nov 12.
Article
en En
| MEDLINE
| ID: mdl-10558992
ABSTRACT
Before fertilization, vertebrate eggs are arrested in metaphase of meiosis II by cytostatic factor (CSF), an activity that requires activation of the mitogen-activated protein kinase (MAPK) pathway. To investigate whether CSF arrest is mediated by the protein kinase p90Rsk, which is phosphorylated and activated by MAPK, a constitutively activated (CA) form of Rsk was expressed in Xenopus embryos. Expression of CA Rsk resulted in cleavage arrest, and cytological analysis showed that arrested blastomeres were in M phase with prominent spindles characteristic of meiotic metaphase. Thus, Rsk appears to be the mediator of MAPK-dependent CSF arrest in vertebrate unfertilized eggs.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Blastómeros
/
Proteínas Quinasas S6 Ribosómicas
/
Proteínas Quinasas Activadas por Mitógenos
/
Sistema de Señalización de MAP Quinasas
/
Metafase
Límite:
Animals
Idioma:
En
Revista:
Science
Año:
1999
Tipo del documento:
Article
País de afiliación:
Estados Unidos