Identification of a LFA-1 region involved in the HIV-1-induced syncytia formation through phage-display technology.
Eur J Immunol
; 31(1): 57-63, 2001 Jan.
Article
en En
| MEDLINE
| ID: mdl-11169438
We have identified a peptide region on CD18 molecule (the beta subunit of the LFA-1 molecule) involved in syncytia formation of HIV-1-infected lymphocytes. Several phage clones mimicking an epitope of the CD18 cell-surface determinant were isolated from two 9-mer random peptide phage-displayed libraries via their binding to the CD18-specific monoclonal antibody (mAb) MHM23, which in in vitro assay inhibits syncytia formation in HIV-1-infected cells. The peptide sequences displayed on phages that blocked immunolabeling of this mAb on LFA-1-expressing cells were used to identify the epitope recognized by mAb MHM23 by sequence comparison. On the basis of this analysis, two peptides which inhibited syncytia formation in HIV-1-infected cells in vitro were synthesized, thus confirming that they mimic a CD18 domain that is involved in this phenomenon. The results here presented highlight the potential of phage-display technology for the study of biological processes at the basis of virus infection, but also suggest new approaches for the therapy of AIDS.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Bacteriófagos
/
Antígeno-1 Asociado a Función de Linfocito
/
VIH-1
/
Mapeo Epitopo
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Eur J Immunol
Año:
2001
Tipo del documento:
Article
País de afiliación:
Italia