[Optimization of the conditions for RAPD-PCR of Candida spp. and Cryptococcus spp]. / Optimización de las condiciones RAPD-PCR en Candida spp. y Cryptococcus spp.
Rev Iberoam Micol
; 18(2): 65-9, 2001 Jun.
Article
en Es
| MEDLINE
| ID: mdl-15487909
ABSTRACT
In this study, we determined the optimal RAPD amplification conditions to obtain genetic molecular markers for the rapid and accurate identification of Cryptococcus spp. and Candida spp. The following parameters are modified template DNA, DNA polymerase, magnesium cloride and primer concentration; denaturation, annealing and extension time, temperature of annealing and thermal cycles. After the optimization, reliable and reproducible RAPD patterns are obtained.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Idioma:
Es
Revista:
Rev Iberoam Micol
Asunto de la revista:
MICROBIOLOGIA
Año:
2001
Tipo del documento:
Article