[Expressions and activities of Rho GTPases in hypoxia and its relationship with tumor angiogenesis].
Zhonghua Zhong Liu Za Zhi
; 26(9): 517-20, 2004 Sep.
Article
en Zh
| MEDLINE
| ID: mdl-15555278
ABSTRACT
OBJECTIVE:
To study the expressions and activities of Rho GTPases in hypoxia and its relationship with tumor angiogenesis.METHODS:
Three tumor cell lines were used in this study gastric cancer cell lines AGS, SGC7901 and hepatocellular carcinoma cell line HepG2. Expression level of Rac1 mRNA was detected by semi-quantitative RT-PCR. Activity of Rac1 was determined by pull-down assay and expression of HIF-1alpha, VEGF, p53 and PTEN protein was detected by Westernblot.RESULTS:
The expression level of Rac1 mRNA was significantly increased in hypoxia compared to normoxia. Pull-down assay showed that hypoxia-induced activity of Rac1 was elevated in a time-dependent manner and climaxed at 3 hours. The expressions of HIF-1alpha and VEGF protein were up-regulated, while those of PTEN and p53 protein were down-regulated.CONCLUSION:
These results indicate that hypoxia enhances Rac1 expression which might be involved in tumor angiogenesis by reacting with hypoxia-responsive genes.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Neoplasias Gástricas
/
Hepatoblastoma
/
Proteínas de Unión al GTP rho
/
Proteína de Unión al GTP rac1
/
Neoplasias Hepáticas
/
Neovascularización Patológica
Límite:
Humans
Idioma:
Zh
Revista:
Zhonghua Zhong Liu Za Zhi
Año:
2004
Tipo del documento:
Article
País de afiliación:
China