Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT.
BMC Immunol
; 6: 17, 2005 Jul 18.
Article
en En
| MEDLINE
| ID: mdl-16026627
ABSTRACT
BACKGROUND:
Cryopreservation of PBMC and/or overnight shipping of samples are required for many clinical trials, despite their potentially adverse effects upon immune monitoring assays such as MHC-peptide tetramer staining, cytokine flow cytometry (CFC), and ELISPOT. In this study, we compared the performance of these assays on leukapheresed PBMC shipped overnight in medium versus cryopreserved PBMC from matched donors.RESULTS:
Using CMV pp65 peptide pool stimulation or pp65 HLA-A2 tetramer staining, there was significant correlation between shipped and cryopreserved samples for each assay (pCONCLUSION:
We conclude that all three assays show concordant results on shipped versus cryopreserved specimens, when using a peptide-based readout. The assays are also concordant with each other in pair wise comparisons using equivalent antigen systems.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fosfoproteínas
/
Ensayo de Inmunoadsorción Enzimática
/
Leucocitos Mononucleares
/
Criopreservación
/
Antígeno HLA-A2
/
Proteínas de la Matriz Viral
/
Presentación de Antígeno
/
Citomegalovirus
/
Citometría de Flujo
/
Antígenos Virales
Tipo de estudio:
Clinical_trials
/
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
BMC Immunol
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
2005
Tipo del documento:
Article
País de afiliación:
Estados Unidos