Gene expression profile of ewing sarcoma cell lines differing in their EWS-FLI1 fusion type.
J Pediatr Hematol Oncol
; 27(10): 537-42, 2005 Oct.
Article
en En
| MEDLINE
| ID: mdl-16217257
ABSTRACT
The t(11;22)(q24;q12) translocation is present in up to 95% of Ewing tumor patients and results in the formation of an EWS-FLI-1 fusion gene that encodes a chimeric transcription factor. Many alternative forms of EWS-FLI-1 exist because of variations in the location of the EWS and FLI-1 genomic breakpoints. Previous reports have shown that the type 1 fusion is associated with a significantly better prognosis than the other fusion types. It has been suggested that the observed clinical discrepancies result from different transactivation potentials of the various EWS-FLI-1 fusion proteins. In an attempt to identify genes whose expression levels are differentially modulated by structurally different EWS-FLI-1 transcription factors, we have used microarray technology to interrogate 19,000 sequence genes to compare gene expression profile of type 1 or non-type 1 Ewing sarcoma cell lines. Data analysis showed few qualitative differences on gene expression; expression of only 41 genes (0.215% of possible sequences analyzed) differed significantly between Ewing tumor cell lines carrying EWS-FLI-1 fusion type 1 with respect to those with non-type 1 fusion.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Sarcoma de Ewing
/
Neoplasias Óseas
/
Biomarcadores de Tumor
/
Regulación Neoplásica de la Expresión Génica
/
Proteínas de Fusión Oncogénica
/
Perfilación de la Expresión Génica
/
Proteína Proto-Oncogénica c-fli-1
/
Proteínas de Neoplasias
Tipo de estudio:
Prognostic_studies
/
Qualitative_research
Límite:
Humans
Idioma:
En
Revista:
J Pediatr Hematol Oncol
Asunto de la revista:
HEMATOLOGIA
/
NEOPLASIAS
/
PEDIATRIA
Año:
2005
Tipo del documento:
Article
País de afiliación:
España