Array-based profiling of reference-independent methylation status (aPRIMES) identifies frequent promoter methylation and consecutive downregulation of ZIC2 in pediatric medulloblastoma.
Nucleic Acids Res
; 35(7): e51, 2007.
Article
en En
| MEDLINE
| ID: mdl-17344319
ABSTRACT
Existing microarray-based approaches for screening of DNA methylation are hampered by a number of shortcomings, such as the introduction of bias by DNA copy-number imbalances in the test genome and negligence of tissue-specific methylation patterns. We developed a method designated array-based profiling of reference-independent methylation status (aPRIMES) that allows the detection of direct methylation status rather than relative methylation. Array-PRIMES is based on the differential restriction and competitive hybridization of methylated and unmethylated DNA by methylation-specific and methylation-sensitive restriction enzymes, respectively. We demonstrate the accuracy of aPRIMES in detecting the methylation status of CpG islands for different states of methylation. Application of aPRIMES to the DNA from desmoplastic medulloblastomas of monozygotic twins showed strikingly similar methylation profiles. Additional analysis of 18 sporadic medulloblastomas revealed an overall correlation between highly methylated tumors and poor clinical outcome and identified ZIC2 as a frequently methylated gene in pediatric medulloblastoma.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
/
Proteínas Nucleares
/
Neoplasias Cerebelosas
/
Regiones Promotoras Genéticas
/
Metilación de ADN
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Meduloblastoma
Tipo de estudio:
Evaluation_studies
/
Prognostic_studies
Límite:
Child, preschool
/
Humans
Idioma:
En
Revista:
Nucleic Acids Res
Año:
2007
Tipo del documento:
Article
País de afiliación:
Alemania