Growth and regeneration of adult beta cells does not involve specialized progenitors.
Dev Cell
; 12(5): 817-26, 2007 May.
Article
en En
| MEDLINE
| ID: mdl-17488631
ABSTRACT
Cellular progenitors remain poorly characterized in many adult tissues, limited in part by the lack of unbiased techniques to identify progenitors and their progeny. To address this fundamental problem, we developed a novel DNA analog-based lineage-tracing technique to detect multiple rounds of cell division in vivo. Here, we apply this technique to determine the adult lineage mechanism of the insulin-secreting beta cells of pancreatic islets, an important unresolved question in diabetes research. As expected, gastrointestinal and skin epithelia involve specialized progenitors that repeatedly divide to give rise to postmitotic cells. In contrast, specialized progenitors do not contribute to adult beta cells, not even during acute beta cell regeneration. Instead, beta cells are the products of uniform self-renewal, slowed by a replication refractory period that prevents beta cells from immediately redividing. Our approach provides unbiased resolution of previously inaccessible developmental niches and can elucidate lineage mechanisms without candidate markers.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Regeneración
/
Células Secretoras de Insulina
Límite:
Animals
Idioma:
En
Revista:
Dev Cell
Asunto de la revista:
EMBRIOLOGIA
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos