Erythrocytes counteract the negative effects of female ageing on mouse preimplantation embryo development and blastocyst formation.
Hum Reprod
; 23(9): 2080-5, 2008 Sep.
Article
en En
| MEDLINE
| ID: mdl-18583333
ABSTRACT
BACKGROUND:
The low developmental competence of embryos from ageing females remains an enigma; it is presumably attributable to oxidative stress. A number of antioxidant mechanisms exist in the erythrocyte and these have been investigated in other cells and tissues. However, very few studies have reported the effects of erythrocyte supplementation on developmental competence in ageing embryos.METHODS:
In Experiment 1, IVF embryos from young (7-10 weeks) mice were cultured in medium supplemented with an oxidizing agent, hypoxanthine/xanthine oxidase, in the presence and absence of erythrocytes. In Experiment 2, the development of embryos derived from young and ageing (40-50 weeks) female mice was assessed in the presence and absence of erythrocytes.RESULTS:
In Experiment 1, the presence of hypoxanthine/xanthine oxidase significantly inhibited embryo development (P < 0.0001). Erythrocyte supplementation clearly overcame the detrimental effects in a dose-related manner. In Experiment 2, in the absence of erythrocytes, developmental competence was significantly lower in embryos from ageing females than in those from young females (P < 0.01). However, in ageing females, the supplementation of erythrocytes significantly promoted the development of embryos to the blastocyst stage (51.1% versus 77.3%; P < 0.01).CONCLUSIONS:
Supplementation with erythrocytes can counteract the negative effect of maternal ageing on embryo development and blastocyst formation.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Blastocisto
/
Desarrollo Embrionario
/
Eritrocitos
Límite:
Animals
Idioma:
En
Revista:
Hum Reprod
Asunto de la revista:
MEDICINA REPRODUTIVA
Año:
2008
Tipo del documento:
Article
País de afiliación:
Japón