Caenorhabditis elegans mutant allele identification by whole-genome sequencing.
Nat Methods
; 5(10): 865-7, 2008 Oct.
Article
en En
| MEDLINE
| ID: mdl-18677319
ABSTRACT
Identification of the molecular lesion in Caenorhabditis elegans mutants isolated through forward genetic screens usually involves time-consuming genetic mapping. We used Illumina deep sequencing technology to sequence a complete, mutant C. elegans genome and thus pinpointed a single-nucleotide mutation in the genome that affects a neuronal cell fate decision. This constitutes a proof-of-principle for using whole-genome sequencing to analyze C. elegans mutants.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Caenorhabditis elegans
/
Alelos
/
Genoma de los Helmintos
/
Mutación
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2008
Tipo del documento:
Article
País de afiliación:
Estados Unidos