Second-site mutations in Borna disease virus overexpressing viral accessory protein X.
J Gen Virol
; 90(Pt 8): 1932-1936, 2009 Aug.
Article
en En
| MEDLINE
| ID: mdl-19420156
ABSTRACT
The X protein of Borna disease virus (BDV) is an essential factor that regulates viral polymerase activity and inhibits apoptosis of persistently infected cells. We observed that a BDV mutant which carries an additional X gene replicated well in cell culture only after acquiring second-site mutations that selectively reduced expression of the endogenous X gene. In rat brains, the virus acquired additional mutations which inactivated the ectopic X gene or altered the sequence of X. These results demonstrate that BDV readily acquires mutations if strong selection pressure is applied. They further indicate that fine-tuning of X expression determines viral fitness.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Virus de la Enfermedad de Borna
/
Regulación Viral de la Expresión Génica
/
Dosificación de Gen
/
Mutación Missense
/
Proteínas Reguladoras y Accesorias Virales
Límite:
Animals
Idioma:
En
Revista:
J Gen Virol
Año:
2009
Tipo del documento:
Article
País de afiliación:
Alemania