Atomic-level mapping of antibody epitopes on a GPCR.
J Am Chem Soc
; 131(20): 6952-4, 2009 May 27.
Article
en En
| MEDLINE
| ID: mdl-19453194
ABSTRACT
Epitopes that define the immunodominant regions of conformationally complex integral membrane proteins have been difficult to reliably delineate. Here, a high-throughput approach termed shotgun mutagenesis was used to map the binding epitopes of five different monoclonal antibodies targeting the GPCR CCR5. The amino acids, and in some cases the atoms, that comprise the critical contact points of each epitope were identified, defining the immunodominant structures of this GPCR and their physicochemistry.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Epítopos Inmunodominantes
/
Mapeo Epitopo
/
Receptores CCR5
/
Anticuerpos Monoclonales
Idioma:
En
Revista:
J Am Chem Soc
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos