[UP-PCR diversity analysis of Fusarium population isolated from greenhouse melon soils].
Ying Yong Sheng Tai Xue Bao
; 20(4): 857-62, 2009 Apr.
Article
en Zh
| MEDLINE
| ID: mdl-19565767
ABSTRACT
A total of 112 Fusarium isolates were obtained from 36 soil samples collected from the greenhouse melon fields of Liaoning Province, among which, 11 species were identified by traditional morphological classification and rDNA sequence analysis. Universally Primed PCR (UP-PCR) was conducted to analyze the 25 strains of test Fusarium isolates and 3 strains of positive control Fusarium isolates. The results indicated that a total of 73 bands appeared after amplification by using 6 primers, and 66 bands (90.4%) were polymorphic. The isolates were clustered into eight groups at the similarity of 0.736 by cluster analysis, among which, 14 isolates were clustered into one group. It was concluded that UP-PCR could present the genetic relationship and difference among Fusarium strains, being able to be used as an assistant method for Fusarium classification.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Microbiología del Suelo
/
Cucurbitaceae
/
Biodiversidad
/
Fusarium
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Ying Yong Sheng Tai Xue Bao
Asunto de la revista:
SAUDE AMBIENTAL
Año:
2009
Tipo del documento:
Article
País de afiliación:
China