[Effects of C-reactive protein on chemotaxis ability of monocytes in vitro].

ABSTRACT

OBJECTIVE: To investigate the effects of C-reactive protein (CRP) on monocytes chemotaxis ability in vitro. METHODS: Transwell chemotaxis assay was used to evaluate the changes of chemotactic ability of THP-1 monocytes in each group treated with CRP in different concentration. RESULTS: CRP increased the number of attracted monocytes in response to MCP-1 (monocyte chemoattractant protein-1). When treated with CRP concentration at 2 microg x mL(-1), the number of chemotactic monocytes increased (P < 0.05). The number of attracted monocytes increased as CRP concentration was elevated (P < 0.05). CONCLUSION: CRP can increase chemotactic ability of THP-1 monocytes in concentration dependent manner.