Proteolytic activity in Fasciola hepatica is reduced by the administration of cathepsin L mimotopes.
J Helminthol
; 85(1): 51-5, 2011 Mar.
Article
en En
| MEDLINE
| ID: mdl-20416126
ABSTRACT
The objective of this study was to assess the proteolytic activity of Fasciola hepatica cathepsins in liver sections from mice vaccinated with phage clones of cathepsin L mimotopes, using the film in situ zymography technique. Female BALB/c mice were immunized three times with 2.5 x 10¹¹ phage particles without adjuvant. Animals vaccinated with phage clones produced high titres of anti-mimotope antibodies and a significant reduction in fluke burden was observed following challenge with metacercariae of F. hepatica. The proteolytic activity in hepatic tissue was reduced after the immunization with phage clones.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Anticuerpos Antihelmínticos
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Vacunas
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Fasciola hepatica
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Fascioliasis
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Catepsina L
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Hígado
Límite:
Animals
Idioma:
En
Revista:
J Helminthol
Año:
2011
Tipo del documento:
Article